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CD163 and CCR7 as markers for macrophage polarization in lung cancer microenvironment
INTRODUCTION: M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype in the local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF). AIM OF THE STUDY: To find differences between BALF from lung...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050058/ https://www.ncbi.nlm.nih.gov/pubmed/32140052 http://dx.doi.org/10.5114/ceji.2019.92795 |
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author | KWIECIEŃ, IWONA POLUBIEC-KOWNACKA, MAŁGORZATA DZIEDZIC, DARIUSZ WOŁOSZ, DOMINIKA RZEPECKI, PIOTR DOMAGAŁA-KULAWIK, JOANNA |
author_facet | KWIECIEŃ, IWONA POLUBIEC-KOWNACKA, MAŁGORZATA DZIEDZIC, DARIUSZ WOŁOSZ, DOMINIKA RZEPECKI, PIOTR DOMAGAŁA-KULAWIK, JOANNA |
author_sort | KWIECIEŃ, IWONA |
collection | PubMed |
description | INTRODUCTION: M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype in the local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF). AIM OF THE STUDY: To find differences between BALF from lung affected by cancer (clBALF) and hlBALF from the opposite, healthy lung, as a control, from the same patient, regarding their individual macrophage polarization and their correlation with IL-10 and TGF-β. MATERIAL AND METHODS: Eighteen patients with confirmed lung cancer were investigated. Macrophage subtyping was performed by immunofluorescence with antibodies anti-CCR7 and CD163 (M1 and M2, respectively). RESULTS: We found five populations of macrophages: cells with a single reaction: only for CCR7+ or CD163+, a double reaction (CCR7+CD163+), cells with a stronger CD163 (CCR7(low)CD163+), and cells with a stronger CCR7 (CCR7+CD163(low)). The main population in the clBALF was composed of cells with a phenotype similar to M2 (CCR7(low)CD163+), while in the hlBALF the predominating phenotype was the one similar to M1 (CCR7+CD163(low)). The median proportion of TGF-β1 concentration was higher in the clBALF and hlBALF supernatant than in the serum. CONCLUSIONS: In this study we confirmed the usefulness of the immunofluorescence method with CCR7 and CD163 in the evaluation of BALF macrophage polarization in lung cancer. |
format | Online Article Text |
id | pubmed-7050058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-70500582020-03-05 CD163 and CCR7 as markers for macrophage polarization in lung cancer microenvironment KWIECIEŃ, IWONA POLUBIEC-KOWNACKA, MAŁGORZATA DZIEDZIC, DARIUSZ WOŁOSZ, DOMINIKA RZEPECKI, PIOTR DOMAGAŁA-KULAWIK, JOANNA Cent Eur J Immunol Clinical Immunology INTRODUCTION: M2 macrophages are predominant in the immune infiltrates of resected tumours, but little is known about macrophage phenotype in the local lung cancer environment, which may be evaluated by bronchoalveolar lavage fluid (BALF). AIM OF THE STUDY: To find differences between BALF from lung affected by cancer (clBALF) and hlBALF from the opposite, healthy lung, as a control, from the same patient, regarding their individual macrophage polarization and their correlation with IL-10 and TGF-β. MATERIAL AND METHODS: Eighteen patients with confirmed lung cancer were investigated. Macrophage subtyping was performed by immunofluorescence with antibodies anti-CCR7 and CD163 (M1 and M2, respectively). RESULTS: We found five populations of macrophages: cells with a single reaction: only for CCR7+ or CD163+, a double reaction (CCR7+CD163+), cells with a stronger CD163 (CCR7(low)CD163+), and cells with a stronger CCR7 (CCR7+CD163(low)). The main population in the clBALF was composed of cells with a phenotype similar to M2 (CCR7(low)CD163+), while in the hlBALF the predominating phenotype was the one similar to M1 (CCR7+CD163(low)). The median proportion of TGF-β1 concentration was higher in the clBALF and hlBALF supernatant than in the serum. CONCLUSIONS: In this study we confirmed the usefulness of the immunofluorescence method with CCR7 and CD163 in the evaluation of BALF macrophage polarization in lung cancer. Termedia Publishing House 2020-01-20 2019 /pmc/articles/PMC7050058/ /pubmed/32140052 http://dx.doi.org/10.5114/ceji.2019.92795 Text en Copyright © 2019 Termedia http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/) |
spellingShingle | Clinical Immunology KWIECIEŃ, IWONA POLUBIEC-KOWNACKA, MAŁGORZATA DZIEDZIC, DARIUSZ WOŁOSZ, DOMINIKA RZEPECKI, PIOTR DOMAGAŁA-KULAWIK, JOANNA CD163 and CCR7 as markers for macrophage polarization in lung cancer microenvironment |
title | CD163 and CCR7 as markers for macrophage polarization in lung cancer microenvironment |
title_full | CD163 and CCR7 as markers for macrophage polarization in lung cancer microenvironment |
title_fullStr | CD163 and CCR7 as markers for macrophage polarization in lung cancer microenvironment |
title_full_unstemmed | CD163 and CCR7 as markers for macrophage polarization in lung cancer microenvironment |
title_short | CD163 and CCR7 as markers for macrophage polarization in lung cancer microenvironment |
title_sort | cd163 and ccr7 as markers for macrophage polarization in lung cancer microenvironment |
topic | Clinical Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050058/ https://www.ncbi.nlm.nih.gov/pubmed/32140052 http://dx.doi.org/10.5114/ceji.2019.92795 |
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