Cargando…
Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures
DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force‐tracin...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050086/ https://www.ncbi.nlm.nih.gov/pubmed/32140254 http://dx.doi.org/10.1002/gch2.201900075 |
_version_ | 1783502567453491200 |
---|---|
author | Chen, Xi Tian, Falin Li, Min Xu, Haijiao Cai, Mingjun Li, Qian Zuo, Xiaolei Wang, Hongda Shi, Xinghua Fan, Chunhai Baigude, Huricha Shan, Yuping |
author_facet | Chen, Xi Tian, Falin Li, Min Xu, Haijiao Cai, Mingjun Li, Qian Zuo, Xiaolei Wang, Hongda Shi, Xinghua Fan, Chunhai Baigude, Huricha Shan, Yuping |
author_sort | Chen, Xi |
collection | PubMed |
description | DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force‐tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s(−1). Interestingly, the dynamic parameters are irrelevant to the size of TDNs, while the larger TDNs rotated slightly during the transporting process. Meanwhile, both the results from single‐molecule force tracing and ensemble fluorescence imaging demonstrate that the different size TDNs transmembrane transporting depends on caveolin‐mediated endocytosis. |
format | Online Article Text |
id | pubmed-7050086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70500862020-03-05 Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures Chen, Xi Tian, Falin Li, Min Xu, Haijiao Cai, Mingjun Li, Qian Zuo, Xiaolei Wang, Hongda Shi, Xinghua Fan, Chunhai Baigude, Huricha Shan, Yuping Glob Chall Full Papers DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force‐tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s(−1). Interestingly, the dynamic parameters are irrelevant to the size of TDNs, while the larger TDNs rotated slightly during the transporting process. Meanwhile, both the results from single‐molecule force tracing and ensemble fluorescence imaging demonstrate that the different size TDNs transmembrane transporting depends on caveolin‐mediated endocytosis. John Wiley and Sons Inc. 2019-11-20 /pmc/articles/PMC7050086/ /pubmed/32140254 http://dx.doi.org/10.1002/gch2.201900075 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Chen, Xi Tian, Falin Li, Min Xu, Haijiao Cai, Mingjun Li, Qian Zuo, Xiaolei Wang, Hongda Shi, Xinghua Fan, Chunhai Baigude, Huricha Shan, Yuping Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
title | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
title_full | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
title_fullStr | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
title_full_unstemmed | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
title_short | Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures |
title_sort | size‐independent transmembrane transporting of single tetrahedral dna nanostructures |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050086/ https://www.ncbi.nlm.nih.gov/pubmed/32140254 http://dx.doi.org/10.1002/gch2.201900075 |
work_keys_str_mv | AT chenxi sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT tianfalin sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT limin sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT xuhaijiao sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT caimingjun sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT liqian sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT zuoxiaolei sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT wanghongda sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT shixinghua sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT fanchunhai sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT baigudehuricha sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures AT shanyuping sizeindependenttransmembranetransportingofsingletetrahedraldnananostructures |