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Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures

DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force‐tracin...

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Autores principales: Chen, Xi, Tian, Falin, Li, Min, Xu, Haijiao, Cai, Mingjun, Li, Qian, Zuo, Xiaolei, Wang, Hongda, Shi, Xinghua, Fan, Chunhai, Baigude, Huricha, Shan, Yuping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050086/
https://www.ncbi.nlm.nih.gov/pubmed/32140254
http://dx.doi.org/10.1002/gch2.201900075
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author Chen, Xi
Tian, Falin
Li, Min
Xu, Haijiao
Cai, Mingjun
Li, Qian
Zuo, Xiaolei
Wang, Hongda
Shi, Xinghua
Fan, Chunhai
Baigude, Huricha
Shan, Yuping
author_facet Chen, Xi
Tian, Falin
Li, Min
Xu, Haijiao
Cai, Mingjun
Li, Qian
Zuo, Xiaolei
Wang, Hongda
Shi, Xinghua
Fan, Chunhai
Baigude, Huricha
Shan, Yuping
author_sort Chen, Xi
collection PubMed
description DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force‐tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s(−1). Interestingly, the dynamic parameters are irrelevant to the size of TDNs, while the larger TDNs rotated slightly during the transporting process. Meanwhile, both the results from single‐molecule force tracing and ensemble fluorescence imaging demonstrate that the different size TDNs transmembrane transporting depends on caveolin‐mediated endocytosis.
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spelling pubmed-70500862020-03-05 Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures Chen, Xi Tian, Falin Li, Min Xu, Haijiao Cai, Mingjun Li, Qian Zuo, Xiaolei Wang, Hongda Shi, Xinghua Fan, Chunhai Baigude, Huricha Shan, Yuping Glob Chall Full Papers DNA nanostructures have attracted considerable attention as drug delivery carriers. However, the transmembrane kinetics of DNA nanostructures remains less explored. Herein, the dynamic process of transporting single tetrahedral DNA nanostructures (TDNs) is monitored in real time using a force‐tracing technique based on atomic force microscopy. The results show that transporting single TDNs into living HeLa cells need ≈53 pN force and ≈25 ms duration with the average speed of ≈0.6 µm s(−1). Interestingly, the dynamic parameters are irrelevant to the size of TDNs, while the larger TDNs rotated slightly during the transporting process. Meanwhile, both the results from single‐molecule force tracing and ensemble fluorescence imaging demonstrate that the different size TDNs transmembrane transporting depends on caveolin‐mediated endocytosis. John Wiley and Sons Inc. 2019-11-20 /pmc/articles/PMC7050086/ /pubmed/32140254 http://dx.doi.org/10.1002/gch2.201900075 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Chen, Xi
Tian, Falin
Li, Min
Xu, Haijiao
Cai, Mingjun
Li, Qian
Zuo, Xiaolei
Wang, Hongda
Shi, Xinghua
Fan, Chunhai
Baigude, Huricha
Shan, Yuping
Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures
title Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures
title_full Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures
title_fullStr Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures
title_full_unstemmed Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures
title_short Size‐Independent Transmembrane Transporting of Single Tetrahedral DNA Nanostructures
title_sort size‐independent transmembrane transporting of single tetrahedral dna nanostructures
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050086/
https://www.ncbi.nlm.nih.gov/pubmed/32140254
http://dx.doi.org/10.1002/gch2.201900075
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