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Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N(2)O as a reaction gas for in‐line Rb separation
RATIONALE: Strontium isotopes are valuable markers of provenance in a range of disciplines. Limited amounts of Sr in low‐mass samples such as insects mean that conventional Sr isotope analysis precludes their use for geographic origins in many ecological studies or in applications such as biosecurit...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050539/ https://www.ncbi.nlm.nih.gov/pubmed/31756774 http://dx.doi.org/10.1002/rcm.8604 |
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author | Murphy, David Thomas Allen, Charlotte M. Ghidan, Osama Dickson, Andrew Hu, Wan‐Ping Briggs, Ethan Holder, Peter W. Armstrong, Karen F. |
author_facet | Murphy, David Thomas Allen, Charlotte M. Ghidan, Osama Dickson, Andrew Hu, Wan‐Ping Briggs, Ethan Holder, Peter W. Armstrong, Karen F. |
author_sort | Murphy, David Thomas |
collection | PubMed |
description | RATIONALE: Strontium isotopes are valuable markers of provenance in a range of disciplines. Limited amounts of Sr in low‐mass samples such as insects mean that conventional Sr isotope analysis precludes their use for geographic origins in many ecological studies or in applications such as biosecurity. Here we test the viability of using inductively coupled plasma tandem mass spectrometry (ICP‐MS/MS) with N(2)O as a reaction gas for accurately determining Sr isotopes in insects with Sr < 100 ng. METHODS: Strontium isotopes were determined in solution mode using ICP‐MS/MS with 0.14 L/min N(2)O as a reaction gas to convert Sr(+) into SrO(+) for in‐line separation of (87)Sr from (87)Rb. The Sr isotope reference standards NIST SRM 987, NIST SRM 1570a and NIST SRM 1547 were used to assess accuracy and reproducibility. Ten insect species collected from the wild as a proof‐of‐principle application were analysed for Sr concentration and Sr isotopes. RESULTS: Using ICP‐MS/MS we show for the first time that internal mass bias correction of (87)Sr(16)O/(86)Sr(16)O based on (88)Sr(16)O/(86)Sr(16)O works to give for NIST SRM 987 a (87)Sr/(86)Sr ratio of 0.7101 ± 0.012 (RSD = 0.17%) and for NIST SRM 1570a a (87)Sr/(86)Sr ratio of 0.7100 ± 0.009 (RSD = 0.12%), which are within error of the accepted values. The first (87)Sr/(86)Sr ratio of NIST SRM 1547 is 0.7596 ± 0.0014. Strontium analyses were run on 0.8 mL of 0.25–0.5 ppb Sr, which equates to 2–4 ng of Sr. Strontium isotope analysis with a precision of >99.8% can be achieved with in‐line separation of (87)Sr from (87)Rb at least up to solutions with 25 ppb Rb. CONCLUSIONS: A minimum of 5 mg of insect tissue is required for Sr isotope analysis. This new ICP‐MS/MS method enables Sr isotope analysis in single insects, allowing population‐scale studies to be feasible and making possible applications with time‐critical uses such as biosecurity. |
format | Online Article Text |
id | pubmed-7050539 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70505392020-03-09 Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N(2)O as a reaction gas for in‐line Rb separation Murphy, David Thomas Allen, Charlotte M. Ghidan, Osama Dickson, Andrew Hu, Wan‐Ping Briggs, Ethan Holder, Peter W. Armstrong, Karen F. Rapid Commun Mass Spectrom Research Articles RATIONALE: Strontium isotopes are valuable markers of provenance in a range of disciplines. Limited amounts of Sr in low‐mass samples such as insects mean that conventional Sr isotope analysis precludes their use for geographic origins in many ecological studies or in applications such as biosecurity. Here we test the viability of using inductively coupled plasma tandem mass spectrometry (ICP‐MS/MS) with N(2)O as a reaction gas for accurately determining Sr isotopes in insects with Sr < 100 ng. METHODS: Strontium isotopes were determined in solution mode using ICP‐MS/MS with 0.14 L/min N(2)O as a reaction gas to convert Sr(+) into SrO(+) for in‐line separation of (87)Sr from (87)Rb. The Sr isotope reference standards NIST SRM 987, NIST SRM 1570a and NIST SRM 1547 were used to assess accuracy and reproducibility. Ten insect species collected from the wild as a proof‐of‐principle application were analysed for Sr concentration and Sr isotopes. RESULTS: Using ICP‐MS/MS we show for the first time that internal mass bias correction of (87)Sr(16)O/(86)Sr(16)O based on (88)Sr(16)O/(86)Sr(16)O works to give for NIST SRM 987 a (87)Sr/(86)Sr ratio of 0.7101 ± 0.012 (RSD = 0.17%) and for NIST SRM 1570a a (87)Sr/(86)Sr ratio of 0.7100 ± 0.009 (RSD = 0.12%), which are within error of the accepted values. The first (87)Sr/(86)Sr ratio of NIST SRM 1547 is 0.7596 ± 0.0014. Strontium analyses were run on 0.8 mL of 0.25–0.5 ppb Sr, which equates to 2–4 ng of Sr. Strontium isotope analysis with a precision of >99.8% can be achieved with in‐line separation of (87)Sr from (87)Rb at least up to solutions with 25 ppb Rb. CONCLUSIONS: A minimum of 5 mg of insect tissue is required for Sr isotope analysis. This new ICP‐MS/MS method enables Sr isotope analysis in single insects, allowing population‐scale studies to be feasible and making possible applications with time‐critical uses such as biosecurity. John Wiley and Sons Inc. 2020-02-05 2020-03-15 /pmc/articles/PMC7050539/ /pubmed/31756774 http://dx.doi.org/10.1002/rcm.8604 Text en © 2019 The Authors. Rapid Communications in Mass Spectrometry published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Murphy, David Thomas Allen, Charlotte M. Ghidan, Osama Dickson, Andrew Hu, Wan‐Ping Briggs, Ethan Holder, Peter W. Armstrong, Karen F. Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N(2)O as a reaction gas for in‐line Rb separation |
title | Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N(2)O as a reaction gas for in‐line Rb separation |
title_full | Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N(2)O as a reaction gas for in‐line Rb separation |
title_fullStr | Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N(2)O as a reaction gas for in‐line Rb separation |
title_full_unstemmed | Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N(2)O as a reaction gas for in‐line Rb separation |
title_short | Analysing Sr isotopes in low‐Sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using N(2)O as a reaction gas for in‐line Rb separation |
title_sort | analysing sr isotopes in low‐sr samples such as single insects with inductively coupled plasma tandem mass spectrometry using n(2)o as a reaction gas for in‐line rb separation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7050539/ https://www.ncbi.nlm.nih.gov/pubmed/31756774 http://dx.doi.org/10.1002/rcm.8604 |
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