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CD11c(+) B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors

CD11c(+) B cells have been reported to be increased in autoimmune diseases, but they are detected in the blood of healthy individuals as well. We aimed to characterize CD11c(+) B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays. Here, we report that CD...

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Autores principales: Golinski, Marie-Laure, Demeules, Mélanie, Derambure, Céline, Riou, Gaetan, Maho-Vaillant, Maud, Boyer, Olivier, Joly, Pascal, Calbo, Sébastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7051942/
https://www.ncbi.nlm.nih.gov/pubmed/32158442
http://dx.doi.org/10.3389/fimmu.2020.00032
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author Golinski, Marie-Laure
Demeules, Mélanie
Derambure, Céline
Riou, Gaetan
Maho-Vaillant, Maud
Boyer, Olivier
Joly, Pascal
Calbo, Sébastien
author_facet Golinski, Marie-Laure
Demeules, Mélanie
Derambure, Céline
Riou, Gaetan
Maho-Vaillant, Maud
Boyer, Olivier
Joly, Pascal
Calbo, Sébastien
author_sort Golinski, Marie-Laure
collection PubMed
description CD11c(+) B cells have been reported to be increased in autoimmune diseases, but they are detected in the blood of healthy individuals as well. We aimed to characterize CD11c(+) B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays. Here, we report that CD11c(+) B cells are a distinct subpopulation of B cells, enriched in the memory subpopulation even if their phenotype is heterogeneous, with overexpression of genes involved in B-cell activation and differentiation as well as in antigen presentation. Upon activation, CD11c(+) B cells can differentiate into antibody-secreting cells, and CD11c could be upregulated in CD11c(−) B cells by B-cell receptor activation. Finally, we show that patients with pemphigus, an autoimmune disease mediated by B cells, have a decreased frequency of CD11c(+) B cell after treatment, relative to baseline. Our findings show that CD11c(+) B cells are mainly memory B cells prone to differentiate into antibody secreting cells that accumulate with age, independently of gender.
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spelling pubmed-70519422020-03-10 CD11c(+) B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors Golinski, Marie-Laure Demeules, Mélanie Derambure, Céline Riou, Gaetan Maho-Vaillant, Maud Boyer, Olivier Joly, Pascal Calbo, Sébastien Front Immunol Immunology CD11c(+) B cells have been reported to be increased in autoimmune diseases, but they are detected in the blood of healthy individuals as well. We aimed to characterize CD11c(+) B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays. Here, we report that CD11c(+) B cells are a distinct subpopulation of B cells, enriched in the memory subpopulation even if their phenotype is heterogeneous, with overexpression of genes involved in B-cell activation and differentiation as well as in antigen presentation. Upon activation, CD11c(+) B cells can differentiate into antibody-secreting cells, and CD11c could be upregulated in CD11c(−) B cells by B-cell receptor activation. Finally, we show that patients with pemphigus, an autoimmune disease mediated by B cells, have a decreased frequency of CD11c(+) B cell after treatment, relative to baseline. Our findings show that CD11c(+) B cells are mainly memory B cells prone to differentiate into antibody secreting cells that accumulate with age, independently of gender. Frontiers Media S.A. 2020-02-25 /pmc/articles/PMC7051942/ /pubmed/32158442 http://dx.doi.org/10.3389/fimmu.2020.00032 Text en Copyright © 2020 Golinski, Demeules, Derambure, Riou, Maho-Vaillant, Boyer, Joly and Calbo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Golinski, Marie-Laure
Demeules, Mélanie
Derambure, Céline
Riou, Gaetan
Maho-Vaillant, Maud
Boyer, Olivier
Joly, Pascal
Calbo, Sébastien
CD11c(+) B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors
title CD11c(+) B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors
title_full CD11c(+) B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors
title_fullStr CD11c(+) B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors
title_full_unstemmed CD11c(+) B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors
title_short CD11c(+) B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors
title_sort cd11c(+) b cells are mainly memory cells, precursors of antibody secreting cells in healthy donors
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7051942/
https://www.ncbi.nlm.nih.gov/pubmed/32158442
http://dx.doi.org/10.3389/fimmu.2020.00032
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