Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs

Epstein–Barr virus (EBV) is a ubiquitous human herpes virus, which is implicated in cancer and various autoimmune diseases. This study profiles non-micro small non-coding RNA expression changes induced by latent EBV infection. Using small RNA-Seq, 346 non-micro small RNAs were identified as being si...

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Autores principales: Moss, Lumbini I., Tompkins, Van S., Moss, Walter N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052066/
https://www.ncbi.nlm.nih.gov/pubmed/32154466
http://dx.doi.org/10.1016/j.ncrna.2020.02.002
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author Moss, Lumbini I.
Tompkins, Van S.
Moss, Walter N.
author_facet Moss, Lumbini I.
Tompkins, Van S.
Moss, Walter N.
author_sort Moss, Lumbini I.
collection PubMed
description Epstein–Barr virus (EBV) is a ubiquitous human herpes virus, which is implicated in cancer and various autoimmune diseases. This study profiles non-micro small non-coding RNA expression changes induced by latent EBV infection. Using small RNA-Seq, 346 non-micro small RNAs were identified as being significantly differentially expressed between EBV(+) BJAB-B1 and EBV(−) BJAB cell lines. Select small RNA expression changes were experimentally validated in the BJAB-B1 cell line as well as the EBV-infected Raji and Jijoye cell lines. This latter analysis recapitulated the previously identified induction of vault RNA1, while also finding novel evidence for the deregulation of several tRNAs and a snoRNA.
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spelling pubmed-70520662020-03-09 Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs Moss, Lumbini I. Tompkins, Van S. Moss, Walter N. Noncoding RNA Res Article Epstein–Barr virus (EBV) is a ubiquitous human herpes virus, which is implicated in cancer and various autoimmune diseases. This study profiles non-micro small non-coding RNA expression changes induced by latent EBV infection. Using small RNA-Seq, 346 non-micro small RNAs were identified as being significantly differentially expressed between EBV(+) BJAB-B1 and EBV(−) BJAB cell lines. Select small RNA expression changes were experimentally validated in the BJAB-B1 cell line as well as the EBV-infected Raji and Jijoye cell lines. This latter analysis recapitulated the previously identified induction of vault RNA1, while also finding novel evidence for the deregulation of several tRNAs and a snoRNA. KeAi Publishing 2020-02-21 /pmc/articles/PMC7052066/ /pubmed/32154466 http://dx.doi.org/10.1016/j.ncrna.2020.02.002 Text en © 2020 Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Moss, Lumbini I.
Tompkins, Van S.
Moss, Walter N.
Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs
title Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs
title_full Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs
title_fullStr Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs
title_full_unstemmed Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs
title_short Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs
title_sort differential expression analysis comparing ebv uninfected to infected human cell lines identifies induced non-micro small non-coding rnas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052066/
https://www.ncbi.nlm.nih.gov/pubmed/32154466
http://dx.doi.org/10.1016/j.ncrna.2020.02.002
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