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Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries
Background and Purpose: Early life microbiota plays a crucial role in human health by acting as a barrier from pathogens' invasion and maintaining the intestinal immune homoeostasis. Altered fecal microbiota (FM) ecology was reported in newborns affected by intestinal ischemia. Our purpose was...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052114/ https://www.ncbi.nlm.nih.gov/pubmed/32158700 http://dx.doi.org/10.3389/fcimb.2020.00059 |
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author | Romani, Lorenza Del Chierico, Federica Chiriaco, Maria Foligno, Silvia Reddel, Sofia Salvatori, Guglielmo Cifaldi, Cristina Faraci, Simona Finocchi, Andrea Rossi, Paolo Bagolan, Pietro D'Argenio, Patrizia Putignani, Lorenza Fusaro, Fabio |
author_facet | Romani, Lorenza Del Chierico, Federica Chiriaco, Maria Foligno, Silvia Reddel, Sofia Salvatori, Guglielmo Cifaldi, Cristina Faraci, Simona Finocchi, Andrea Rossi, Paolo Bagolan, Pietro D'Argenio, Patrizia Putignani, Lorenza Fusaro, Fabio |
author_sort | Romani, Lorenza |
collection | PubMed |
description | Background and Purpose: Early life microbiota plays a crucial role in human health by acting as a barrier from pathogens' invasion and maintaining the intestinal immune homoeostasis. Altered fecal microbiota (FM) ecology was reported in newborns affected by intestinal ischemia. Our purpose was to describe, in these patients, the FM, the mucosal microbiota (MM) and the mucosal immunity. Methods: Fourteen newborns underwent intestinal resection because of intestinal ischemia. FM and MM were determined through targeted-metagenomics, diversity assignment and Kruskal-Wallis analyses of Operational taxonomic units (OTUs). The mucosal immune cells were analyzed through cytofluorimetry. Results and Conclusion: Based on the severity intestinal injueris we identified two groups: extensive (EII) and focal intestinal ischemia (FII). FM and MM varied in EII and FII groups, showing in the EII group the predominance of Proteobacteria and Enterobacteriaceae and the reduction of Bacteroidetes and Verrucomicrobia for both microbiota. The MM was characterized by a statistically significant reduction of Bacteroides, Lachnospiraceae and Ruminococcaceae and by a higher diversity in the EII compared to FII group. FM showed a prevalence of Proteobacteria, while the Shannon index was lower in the EII compared to FII group. An overall increment in B- and T-lymphocytes and Natural killer (NK) T-like cells was found for EII mucosal samples associated to an increment of TNF-α and INF-γ expressing cells, compared to FII group. FM and MM carry specific signatures of intestinal ischemic lesions. Further research may be crucial to address the role of specific taxa in EII, expecially with reference to inflammation grade and ischemia extension. |
format | Online Article Text |
id | pubmed-7052114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70521142020-03-10 Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries Romani, Lorenza Del Chierico, Federica Chiriaco, Maria Foligno, Silvia Reddel, Sofia Salvatori, Guglielmo Cifaldi, Cristina Faraci, Simona Finocchi, Andrea Rossi, Paolo Bagolan, Pietro D'Argenio, Patrizia Putignani, Lorenza Fusaro, Fabio Front Cell Infect Microbiol Cellular and Infection Microbiology Background and Purpose: Early life microbiota plays a crucial role in human health by acting as a barrier from pathogens' invasion and maintaining the intestinal immune homoeostasis. Altered fecal microbiota (FM) ecology was reported in newborns affected by intestinal ischemia. Our purpose was to describe, in these patients, the FM, the mucosal microbiota (MM) and the mucosal immunity. Methods: Fourteen newborns underwent intestinal resection because of intestinal ischemia. FM and MM were determined through targeted-metagenomics, diversity assignment and Kruskal-Wallis analyses of Operational taxonomic units (OTUs). The mucosal immune cells were analyzed through cytofluorimetry. Results and Conclusion: Based on the severity intestinal injueris we identified two groups: extensive (EII) and focal intestinal ischemia (FII). FM and MM varied in EII and FII groups, showing in the EII group the predominance of Proteobacteria and Enterobacteriaceae and the reduction of Bacteroidetes and Verrucomicrobia for both microbiota. The MM was characterized by a statistically significant reduction of Bacteroides, Lachnospiraceae and Ruminococcaceae and by a higher diversity in the EII compared to FII group. FM showed a prevalence of Proteobacteria, while the Shannon index was lower in the EII compared to FII group. An overall increment in B- and T-lymphocytes and Natural killer (NK) T-like cells was found for EII mucosal samples associated to an increment of TNF-α and INF-γ expressing cells, compared to FII group. FM and MM carry specific signatures of intestinal ischemic lesions. Further research may be crucial to address the role of specific taxa in EII, expecially with reference to inflammation grade and ischemia extension. Frontiers Media S.A. 2020-02-25 /pmc/articles/PMC7052114/ /pubmed/32158700 http://dx.doi.org/10.3389/fcimb.2020.00059 Text en Copyright © 2020 Romani, Del Chierico, Chiriaco, Foligno, Reddel, Salvatori, Cifaldi, Faraci, Finocchi, Rossi, Bagolan, D'Argenio, Putignani and Fusaro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Romani, Lorenza Del Chierico, Federica Chiriaco, Maria Foligno, Silvia Reddel, Sofia Salvatori, Guglielmo Cifaldi, Cristina Faraci, Simona Finocchi, Andrea Rossi, Paolo Bagolan, Pietro D'Argenio, Patrizia Putignani, Lorenza Fusaro, Fabio Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries |
title | Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries |
title_full | Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries |
title_fullStr | Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries |
title_full_unstemmed | Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries |
title_short | Gut Mucosal and Fecal Microbiota Profiling Combined to Intestinal Immune System in Neonates Affected by Intestinal Ischemic Injuries |
title_sort | gut mucosal and fecal microbiota profiling combined to intestinal immune system in neonates affected by intestinal ischemic injuries |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052114/ https://www.ncbi.nlm.nih.gov/pubmed/32158700 http://dx.doi.org/10.3389/fcimb.2020.00059 |
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