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Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to...

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Autores principales: Romero Victorica, Matias, Soria, Marcelo A., Batista-García, Ramón Alberto, Ceja-Navarro, Javier A., Vikram, Surendra, Ortiz, Maximiliano, Ontañon, Ornella, Ghio, Silvina, Martínez-Ávila, Liliana, Quintero García, Omar Jasiel, Etcheverry, Clara, Campos, Eleonora, Cowan, Donald, Arneodo, Joel, Talia, Paola M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052144/
https://www.ncbi.nlm.nih.gov/pubmed/32123275
http://dx.doi.org/10.1038/s41598-020-60850-5
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author Romero Victorica, Matias
Soria, Marcelo A.
Batista-García, Ramón Alberto
Ceja-Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
Martínez-Ávila, Liliana
Quintero García, Omar Jasiel
Etcheverry, Clara
Campos, Eleonora
Cowan, Donald
Arneodo, Joel
Talia, Paola M.
author_facet Romero Victorica, Matias
Soria, Marcelo A.
Batista-García, Ramón Alberto
Ceja-Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
Martínez-Ávila, Liliana
Quintero García, Omar Jasiel
Etcheverry, Clara
Campos, Eleonora
Cowan, Donald
Arneodo, Joel
Talia, Paola M.
author_sort Romero Victorica, Matias
collection PubMed
description In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
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spelling pubmed-70521442020-03-06 Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes Romero Victorica, Matias Soria, Marcelo A. Batista-García, Ramón Alberto Ceja-Navarro, Javier A. Vikram, Surendra Ortiz, Maximiliano Ontañon, Ornella Ghio, Silvina Martínez-Ávila, Liliana Quintero García, Omar Jasiel Etcheverry, Clara Campos, Eleonora Cowan, Donald Arneodo, Joel Talia, Paola M. Sci Rep Article In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications. Nature Publishing Group UK 2020-03-02 /pmc/articles/PMC7052144/ /pubmed/32123275 http://dx.doi.org/10.1038/s41598-020-60850-5 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Romero Victorica, Matias
Soria, Marcelo A.
Batista-García, Ramón Alberto
Ceja-Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
Martínez-Ávila, Liliana
Quintero García, Omar Jasiel
Etcheverry, Clara
Campos, Eleonora
Cowan, Donald
Arneodo, Joel
Talia, Paola M.
Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_full Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_fullStr Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_full_unstemmed Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_short Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_sort neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052144/
https://www.ncbi.nlm.nih.gov/pubmed/32123275
http://dx.doi.org/10.1038/s41598-020-60850-5
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