Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells

The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exi...

Descripción completa

Detalles Bibliográficos
Autores principales: Wagner, Magdalena, Yoshihara, Masahito, Douagi, Iyadh, Damdimopoulos, Anastasios, Panula, Sarita, Petropoulos, Sophie, Lu, Haojiang, Pettersson, Karin, Palm, Kerstin, Katayama, Shintaro, Hovatta, Outi, Kere, Juha, Lanner, Fredrik, Damdimopoulou, Pauliina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052271/
https://www.ncbi.nlm.nih.gov/pubmed/32123174
http://dx.doi.org/10.1038/s41467-020-14936-3
_version_ 1783502835135021056
author Wagner, Magdalena
Yoshihara, Masahito
Douagi, Iyadh
Damdimopoulos, Anastasios
Panula, Sarita
Petropoulos, Sophie
Lu, Haojiang
Pettersson, Karin
Palm, Kerstin
Katayama, Shintaro
Hovatta, Outi
Kere, Juha
Lanner, Fredrik
Damdimopoulou, Pauliina
author_facet Wagner, Magdalena
Yoshihara, Masahito
Douagi, Iyadh
Damdimopoulos, Anastasios
Panula, Sarita
Petropoulos, Sophie
Lu, Haojiang
Pettersson, Karin
Palm, Kerstin
Katayama, Shintaro
Hovatta, Outi
Kere, Juha
Lanner, Fredrik
Damdimopoulou, Pauliina
author_sort Wagner, Magdalena
collection PubMed
description The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. Here, we report single-cell transcriptomes and cell surface antigen profiles of over 24,000 cells from high quality ovarian cortex samples from 21 patients. Our data identify transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Cells captured by DDX4 antibody are perivascular cells, not oogonial stem cells. Our data do not support the existence of germline stem cells in adult human ovaries, thereby reinforcing the dogma of a limited ovarian reserve.
format Online
Article
Text
id pubmed-7052271
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-70522712020-03-04 Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells Wagner, Magdalena Yoshihara, Masahito Douagi, Iyadh Damdimopoulos, Anastasios Panula, Sarita Petropoulos, Sophie Lu, Haojiang Pettersson, Karin Palm, Kerstin Katayama, Shintaro Hovatta, Outi Kere, Juha Lanner, Fredrik Damdimopoulou, Pauliina Nat Commun Article The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. Here, we report single-cell transcriptomes and cell surface antigen profiles of over 24,000 cells from high quality ovarian cortex samples from 21 patients. Our data identify transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Cells captured by DDX4 antibody are perivascular cells, not oogonial stem cells. Our data do not support the existence of germline stem cells in adult human ovaries, thereby reinforcing the dogma of a limited ovarian reserve. Nature Publishing Group UK 2020-03-02 /pmc/articles/PMC7052271/ /pubmed/32123174 http://dx.doi.org/10.1038/s41467-020-14936-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wagner, Magdalena
Yoshihara, Masahito
Douagi, Iyadh
Damdimopoulos, Anastasios
Panula, Sarita
Petropoulos, Sophie
Lu, Haojiang
Pettersson, Karin
Palm, Kerstin
Katayama, Shintaro
Hovatta, Outi
Kere, Juha
Lanner, Fredrik
Damdimopoulou, Pauliina
Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
title Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
title_full Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
title_fullStr Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
title_full_unstemmed Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
title_short Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
title_sort single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052271/
https://www.ncbi.nlm.nih.gov/pubmed/32123174
http://dx.doi.org/10.1038/s41467-020-14936-3
work_keys_str_mv AT wagnermagdalena singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT yoshiharamasahito singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT douagiiyadh singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT damdimopoulosanastasios singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT panulasarita singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT petropoulossophie singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT luhaojiang singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT petterssonkarin singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT palmkerstin singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT katayamashintaro singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT hovattaouti singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT kerejuha singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT lannerfredrik singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells
AT damdimopouloupauliina singlecellanalysisofhumanovariancortexidentifiesdistinctcellpopulationsbutnooogonialstemcells

Ejemplares similares