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Advances in Plant-Derived Scaffold Proteins
Scaffold proteins form critical biomatrices that support cell adhesion and proliferation for regenerative medicine and drug screening. The increasing demand for such applications urges solutions for cost effective and sustainable supplies of hypoallergenic and biocompatible scaffold proteins. Here,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052361/ https://www.ncbi.nlm.nih.gov/pubmed/32161608 http://dx.doi.org/10.3389/fpls.2020.00122 |
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author | Peng, Congyue Annie Kozubowski, Lukasz Marcotte, William R. |
author_facet | Peng, Congyue Annie Kozubowski, Lukasz Marcotte, William R. |
author_sort | Peng, Congyue Annie |
collection | PubMed |
description | Scaffold proteins form critical biomatrices that support cell adhesion and proliferation for regenerative medicine and drug screening. The increasing demand for such applications urges solutions for cost effective and sustainable supplies of hypoallergenic and biocompatible scaffold proteins. Here, we summarize recent efforts in obtaining plant-derived biosynthetic spider silk analogue and the extracellular matrix protein, collagen. Both proteins are composed of a large number of tandem block repeats, which makes production in bacterial hosts challenging. Furthermore, post-translational modification of collagen is essential for its function which requires co-transformation of multiple copies of human prolyl 4-hydroxylase. We discuss our perspectives on how the GAANTRY system could potentially assist the production of native-sized spider dragline silk proteins and prolyl hydroxylated collagen. The potential of recombinant scaffold proteins in drug delivery and drug discovery is also addressed. |
format | Online Article Text |
id | pubmed-7052361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70523612020-03-11 Advances in Plant-Derived Scaffold Proteins Peng, Congyue Annie Kozubowski, Lukasz Marcotte, William R. Front Plant Sci Plant Science Scaffold proteins form critical biomatrices that support cell adhesion and proliferation for regenerative medicine and drug screening. The increasing demand for such applications urges solutions for cost effective and sustainable supplies of hypoallergenic and biocompatible scaffold proteins. Here, we summarize recent efforts in obtaining plant-derived biosynthetic spider silk analogue and the extracellular matrix protein, collagen. Both proteins are composed of a large number of tandem block repeats, which makes production in bacterial hosts challenging. Furthermore, post-translational modification of collagen is essential for its function which requires co-transformation of multiple copies of human prolyl 4-hydroxylase. We discuss our perspectives on how the GAANTRY system could potentially assist the production of native-sized spider dragline silk proteins and prolyl hydroxylated collagen. The potential of recombinant scaffold proteins in drug delivery and drug discovery is also addressed. Frontiers Media S.A. 2020-02-25 /pmc/articles/PMC7052361/ /pubmed/32161608 http://dx.doi.org/10.3389/fpls.2020.00122 Text en Copyright © 2020 Peng, Kozubowski and Marcotte https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Peng, Congyue Annie Kozubowski, Lukasz Marcotte, William R. Advances in Plant-Derived Scaffold Proteins |
title | Advances in Plant-Derived Scaffold Proteins |
title_full | Advances in Plant-Derived Scaffold Proteins |
title_fullStr | Advances in Plant-Derived Scaffold Proteins |
title_full_unstemmed | Advances in Plant-Derived Scaffold Proteins |
title_short | Advances in Plant-Derived Scaffold Proteins |
title_sort | advances in plant-derived scaffold proteins |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052361/ https://www.ncbi.nlm.nih.gov/pubmed/32161608 http://dx.doi.org/10.3389/fpls.2020.00122 |
work_keys_str_mv | AT pengcongyueannie advancesinplantderivedscaffoldproteins AT kozubowskilukasz advancesinplantderivedscaffoldproteins AT marcottewilliamr advancesinplantderivedscaffoldproteins |