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LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer

Background: Bladder cancer (BC) is one of the most common malignancies world-wide with high morbidity and mortality. Long noncoding RNAs (lncRNAs) are thought to play a critical role in cancer development. LncRNA NRON, a repressor of activated T-cell nuclear factor (NFAT), has been shown to be dysre...

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Autores principales: Xiong, Tiefu, Huang, Chenchen, Li, Jianfa, Yu, Shaokang, Chen, Fangfang, Zhang, Zeng, Zhuang, Chengle, Li, Yawen, Zhuang, Changshui, Huang, Xinbo, Ye, Jing, Zhang, Fangting, Gui, Yaoting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052857/
https://www.ncbi.nlm.nih.gov/pubmed/32194786
http://dx.doi.org/10.7150/jca.37958
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author Xiong, Tiefu
Huang, Chenchen
Li, Jianfa
Yu, Shaokang
Chen, Fangfang
Zhang, Zeng
Zhuang, Chengle
Li, Yawen
Zhuang, Changshui
Huang, Xinbo
Ye, Jing
Zhang, Fangting
Gui, Yaoting
author_facet Xiong, Tiefu
Huang, Chenchen
Li, Jianfa
Yu, Shaokang
Chen, Fangfang
Zhang, Zeng
Zhuang, Chengle
Li, Yawen
Zhuang, Changshui
Huang, Xinbo
Ye, Jing
Zhang, Fangting
Gui, Yaoting
author_sort Xiong, Tiefu
collection PubMed
description Background: Bladder cancer (BC) is one of the most common malignancies world-wide with high morbidity and mortality. Long noncoding RNAs (lncRNAs) are thought to play a critical role in cancer development. LncRNA NRON, a repressor of activated T-cell nuclear factor (NFAT), has been shown to be dysregulated in many cancer types. However, the clinical significance and molecular mechanism of NRON in bladder cancer is still unknown. Methods: The expression levels of NRON in BC tissues and cell lines were tested by RT-qPCR. Survival analysis was performed to detect the correlation between NRON expression and clinical outcomes in patients with BC. The biological role of NRON in BC cells proliferation and metastasis was examined in vitro and in vivo. Results: The expression of NRON was significantly upregulated in BC specimens and cell lines compared with paired adjacent normal tissues and normal cell lines. The upregulation of NRON in bladder cancer patients was significantly associated with the depth of bladder tumor invasion and poor prognosis. Knockdown of NRON inhibited BC cells proliferation, migration, invasion and tumorigenicity. Furthermore, NRON promoted epithelial-mesenchymal transition (EMT) progression, and NRON-induced EZH2 expression contributed to this process. Conclusion: In conclusion, our results suggested that NRON acted as an oncogene and tumor biomarker for BC.
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spelling pubmed-70528572020-03-19 LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer Xiong, Tiefu Huang, Chenchen Li, Jianfa Yu, Shaokang Chen, Fangfang Zhang, Zeng Zhuang, Chengle Li, Yawen Zhuang, Changshui Huang, Xinbo Ye, Jing Zhang, Fangting Gui, Yaoting J Cancer Research Paper Background: Bladder cancer (BC) is one of the most common malignancies world-wide with high morbidity and mortality. Long noncoding RNAs (lncRNAs) are thought to play a critical role in cancer development. LncRNA NRON, a repressor of activated T-cell nuclear factor (NFAT), has been shown to be dysregulated in many cancer types. However, the clinical significance and molecular mechanism of NRON in bladder cancer is still unknown. Methods: The expression levels of NRON in BC tissues and cell lines were tested by RT-qPCR. Survival analysis was performed to detect the correlation between NRON expression and clinical outcomes in patients with BC. The biological role of NRON in BC cells proliferation and metastasis was examined in vitro and in vivo. Results: The expression of NRON was significantly upregulated in BC specimens and cell lines compared with paired adjacent normal tissues and normal cell lines. The upregulation of NRON in bladder cancer patients was significantly associated with the depth of bladder tumor invasion and poor prognosis. Knockdown of NRON inhibited BC cells proliferation, migration, invasion and tumorigenicity. Furthermore, NRON promoted epithelial-mesenchymal transition (EMT) progression, and NRON-induced EZH2 expression contributed to this process. Conclusion: In conclusion, our results suggested that NRON acted as an oncogene and tumor biomarker for BC. Ivyspring International Publisher 2020-01-17 /pmc/articles/PMC7052857/ /pubmed/32194786 http://dx.doi.org/10.7150/jca.37958 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Xiong, Tiefu
Huang, Chenchen
Li, Jianfa
Yu, Shaokang
Chen, Fangfang
Zhang, Zeng
Zhuang, Chengle
Li, Yawen
Zhuang, Changshui
Huang, Xinbo
Ye, Jing
Zhang, Fangting
Gui, Yaoting
LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer
title LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer
title_full LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer
title_fullStr LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer
title_full_unstemmed LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer
title_short LncRNA NRON promotes the proliferation, metastasis and EMT process in bladder cancer
title_sort lncrna nron promotes the proliferation, metastasis and emt process in bladder cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052857/
https://www.ncbi.nlm.nih.gov/pubmed/32194786
http://dx.doi.org/10.7150/jca.37958
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