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MUC1 is associated with TFF2 methylation in gastric cancer
BACKGROUND: Emerging evidence has shown that MUC1 and TFF2 play crucial roles in the H. pylori-infected pathogenesis of gastric cancer (GC). A recent study revealed that H. pylori infection induced obviously increased Tff2 methylation levels in Muc1(−/−) mice compared with controls. However, little...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053135/ https://www.ncbi.nlm.nih.gov/pubmed/32122390 http://dx.doi.org/10.1186/s13148-020-00832-6 |
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author | Ge, Yuqiu Ma, Gaoxiang Liu, Hanting Lin, Yadi Zhang, Gang Du, Mulong Wang, Meilin Chu, Haiyan Zhang, Haiyan Zhang, Zhengdong |
author_facet | Ge, Yuqiu Ma, Gaoxiang Liu, Hanting Lin, Yadi Zhang, Gang Du, Mulong Wang, Meilin Chu, Haiyan Zhang, Haiyan Zhang, Zhengdong |
author_sort | Ge, Yuqiu |
collection | PubMed |
description | BACKGROUND: Emerging evidence has shown that MUC1 and TFF2 play crucial roles in the H. pylori-infected pathogenesis of gastric cancer (GC). A recent study revealed that H. pylori infection induced obviously increased Tff2 methylation levels in Muc1(−/−) mice compared with controls. However, little is known of the molecular mechanism on MUC1 regulating the expression of TFF2. METHODS: We conducted a correlation analysis of MUC1 and TFF2 in public databases and our adjacent GC tissues. Besides, MUC1 overexpression vector or small interfering RNA (siRNA) was transfected into GC cells to assess the change in TFF2 expression. Furthermore, the methylation status of TFF2 was measured by bisulfite sequencing PCR (BSP). RESULTS: The expression of MUC1 was significantly lower in non-cardia and cardia tumor tissues than that in normal tissues. Downregulation of TFF2 expression was also observed in GC tissues. In addition, we found that MUC1 expression was positively associated with TFF2 expression in GC tissues, especially among GC patients with H. pylori infection. Overexpression of MUC1 in BGC-823 and SGC-7901 cell lines substantially increased the TFF2 expression, whereas knockdown of MUC1 reverted this effect. Moreover, MUC1 was negatively related to the methylation of TFF2 in the co-expression analysis. The results of BSP experiments showed that compared with negative vector group, the methylation level of TFF2 was decreased in GC cells transfected with MUC1 overexpression vector. Additionally, survival analysis indicated that GC patients with lower level of MUC1 or TFF2 had a worse outcome. CONCLUSION: Our results indicated that MUC1 was associated with the methylation of TFF2, which may have implications for TFF2 expression in GC. These findings warrant further research toward the underlying mechanism of MUC1 influenced the TFF2 methylation. |
format | Online Article Text |
id | pubmed-7053135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-70531352020-03-10 MUC1 is associated with TFF2 methylation in gastric cancer Ge, Yuqiu Ma, Gaoxiang Liu, Hanting Lin, Yadi Zhang, Gang Du, Mulong Wang, Meilin Chu, Haiyan Zhang, Haiyan Zhang, Zhengdong Clin Epigenetics Research BACKGROUND: Emerging evidence has shown that MUC1 and TFF2 play crucial roles in the H. pylori-infected pathogenesis of gastric cancer (GC). A recent study revealed that H. pylori infection induced obviously increased Tff2 methylation levels in Muc1(−/−) mice compared with controls. However, little is known of the molecular mechanism on MUC1 regulating the expression of TFF2. METHODS: We conducted a correlation analysis of MUC1 and TFF2 in public databases and our adjacent GC tissues. Besides, MUC1 overexpression vector or small interfering RNA (siRNA) was transfected into GC cells to assess the change in TFF2 expression. Furthermore, the methylation status of TFF2 was measured by bisulfite sequencing PCR (BSP). RESULTS: The expression of MUC1 was significantly lower in non-cardia and cardia tumor tissues than that in normal tissues. Downregulation of TFF2 expression was also observed in GC tissues. In addition, we found that MUC1 expression was positively associated with TFF2 expression in GC tissues, especially among GC patients with H. pylori infection. Overexpression of MUC1 in BGC-823 and SGC-7901 cell lines substantially increased the TFF2 expression, whereas knockdown of MUC1 reverted this effect. Moreover, MUC1 was negatively related to the methylation of TFF2 in the co-expression analysis. The results of BSP experiments showed that compared with negative vector group, the methylation level of TFF2 was decreased in GC cells transfected with MUC1 overexpression vector. Additionally, survival analysis indicated that GC patients with lower level of MUC1 or TFF2 had a worse outcome. CONCLUSION: Our results indicated that MUC1 was associated with the methylation of TFF2, which may have implications for TFF2 expression in GC. These findings warrant further research toward the underlying mechanism of MUC1 influenced the TFF2 methylation. BioMed Central 2020-03-02 /pmc/articles/PMC7053135/ /pubmed/32122390 http://dx.doi.org/10.1186/s13148-020-00832-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ge, Yuqiu Ma, Gaoxiang Liu, Hanting Lin, Yadi Zhang, Gang Du, Mulong Wang, Meilin Chu, Haiyan Zhang, Haiyan Zhang, Zhengdong MUC1 is associated with TFF2 methylation in gastric cancer |
title | MUC1 is associated with TFF2 methylation in gastric cancer |
title_full | MUC1 is associated with TFF2 methylation in gastric cancer |
title_fullStr | MUC1 is associated with TFF2 methylation in gastric cancer |
title_full_unstemmed | MUC1 is associated with TFF2 methylation in gastric cancer |
title_short | MUC1 is associated with TFF2 methylation in gastric cancer |
title_sort | muc1 is associated with tff2 methylation in gastric cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053135/ https://www.ncbi.nlm.nih.gov/pubmed/32122390 http://dx.doi.org/10.1186/s13148-020-00832-6 |
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