Cargando…

MUC1 is associated with TFF2 methylation in gastric cancer

BACKGROUND: Emerging evidence has shown that MUC1 and TFF2 play crucial roles in the H. pylori-infected pathogenesis of gastric cancer (GC). A recent study revealed that H. pylori infection induced obviously increased Tff2 methylation levels in Muc1(−/−) mice compared with controls. However, little...

Descripción completa

Detalles Bibliográficos
Autores principales: Ge, Yuqiu, Ma, Gaoxiang, Liu, Hanting, Lin, Yadi, Zhang, Gang, Du, Mulong, Wang, Meilin, Chu, Haiyan, Zhang, Haiyan, Zhang, Zhengdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053135/
https://www.ncbi.nlm.nih.gov/pubmed/32122390
http://dx.doi.org/10.1186/s13148-020-00832-6
_version_ 1783502981701828608
author Ge, Yuqiu
Ma, Gaoxiang
Liu, Hanting
Lin, Yadi
Zhang, Gang
Du, Mulong
Wang, Meilin
Chu, Haiyan
Zhang, Haiyan
Zhang, Zhengdong
author_facet Ge, Yuqiu
Ma, Gaoxiang
Liu, Hanting
Lin, Yadi
Zhang, Gang
Du, Mulong
Wang, Meilin
Chu, Haiyan
Zhang, Haiyan
Zhang, Zhengdong
author_sort Ge, Yuqiu
collection PubMed
description BACKGROUND: Emerging evidence has shown that MUC1 and TFF2 play crucial roles in the H. pylori-infected pathogenesis of gastric cancer (GC). A recent study revealed that H. pylori infection induced obviously increased Tff2 methylation levels in Muc1(−/−) mice compared with controls. However, little is known of the molecular mechanism on MUC1 regulating the expression of TFF2. METHODS: We conducted a correlation analysis of MUC1 and TFF2 in public databases and our adjacent GC tissues. Besides, MUC1 overexpression vector or small interfering RNA (siRNA) was transfected into GC cells to assess the change in TFF2 expression. Furthermore, the methylation status of TFF2 was measured by bisulfite sequencing PCR (BSP). RESULTS: The expression of MUC1 was significantly lower in non-cardia and cardia tumor tissues than that in normal tissues. Downregulation of TFF2 expression was also observed in GC tissues. In addition, we found that MUC1 expression was positively associated with TFF2 expression in GC tissues, especially among GC patients with H. pylori infection. Overexpression of MUC1 in BGC-823 and SGC-7901 cell lines substantially increased the TFF2 expression, whereas knockdown of MUC1 reverted this effect. Moreover, MUC1 was negatively related to the methylation of TFF2 in the co-expression analysis. The results of BSP experiments showed that compared with negative vector group, the methylation level of TFF2 was decreased in GC cells transfected with MUC1 overexpression vector. Additionally, survival analysis indicated that GC patients with lower level of MUC1 or TFF2 had a worse outcome. CONCLUSION: Our results indicated that MUC1 was associated with the methylation of TFF2, which may have implications for TFF2 expression in GC. These findings warrant further research toward the underlying mechanism of MUC1 influenced the TFF2 methylation.
format Online
Article
Text
id pubmed-7053135
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-70531352020-03-10 MUC1 is associated with TFF2 methylation in gastric cancer Ge, Yuqiu Ma, Gaoxiang Liu, Hanting Lin, Yadi Zhang, Gang Du, Mulong Wang, Meilin Chu, Haiyan Zhang, Haiyan Zhang, Zhengdong Clin Epigenetics Research BACKGROUND: Emerging evidence has shown that MUC1 and TFF2 play crucial roles in the H. pylori-infected pathogenesis of gastric cancer (GC). A recent study revealed that H. pylori infection induced obviously increased Tff2 methylation levels in Muc1(−/−) mice compared with controls. However, little is known of the molecular mechanism on MUC1 regulating the expression of TFF2. METHODS: We conducted a correlation analysis of MUC1 and TFF2 in public databases and our adjacent GC tissues. Besides, MUC1 overexpression vector or small interfering RNA (siRNA) was transfected into GC cells to assess the change in TFF2 expression. Furthermore, the methylation status of TFF2 was measured by bisulfite sequencing PCR (BSP). RESULTS: The expression of MUC1 was significantly lower in non-cardia and cardia tumor tissues than that in normal tissues. Downregulation of TFF2 expression was also observed in GC tissues. In addition, we found that MUC1 expression was positively associated with TFF2 expression in GC tissues, especially among GC patients with H. pylori infection. Overexpression of MUC1 in BGC-823 and SGC-7901 cell lines substantially increased the TFF2 expression, whereas knockdown of MUC1 reverted this effect. Moreover, MUC1 was negatively related to the methylation of TFF2 in the co-expression analysis. The results of BSP experiments showed that compared with negative vector group, the methylation level of TFF2 was decreased in GC cells transfected with MUC1 overexpression vector. Additionally, survival analysis indicated that GC patients with lower level of MUC1 or TFF2 had a worse outcome. CONCLUSION: Our results indicated that MUC1 was associated with the methylation of TFF2, which may have implications for TFF2 expression in GC. These findings warrant further research toward the underlying mechanism of MUC1 influenced the TFF2 methylation. BioMed Central 2020-03-02 /pmc/articles/PMC7053135/ /pubmed/32122390 http://dx.doi.org/10.1186/s13148-020-00832-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ge, Yuqiu
Ma, Gaoxiang
Liu, Hanting
Lin, Yadi
Zhang, Gang
Du, Mulong
Wang, Meilin
Chu, Haiyan
Zhang, Haiyan
Zhang, Zhengdong
MUC1 is associated with TFF2 methylation in gastric cancer
title MUC1 is associated with TFF2 methylation in gastric cancer
title_full MUC1 is associated with TFF2 methylation in gastric cancer
title_fullStr MUC1 is associated with TFF2 methylation in gastric cancer
title_full_unstemmed MUC1 is associated with TFF2 methylation in gastric cancer
title_short MUC1 is associated with TFF2 methylation in gastric cancer
title_sort muc1 is associated with tff2 methylation in gastric cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053135/
https://www.ncbi.nlm.nih.gov/pubmed/32122390
http://dx.doi.org/10.1186/s13148-020-00832-6
work_keys_str_mv AT geyuqiu muc1isassociatedwithtff2methylationingastriccancer
AT magaoxiang muc1isassociatedwithtff2methylationingastriccancer
AT liuhanting muc1isassociatedwithtff2methylationingastriccancer
AT linyadi muc1isassociatedwithtff2methylationingastriccancer
AT zhanggang muc1isassociatedwithtff2methylationingastriccancer
AT dumulong muc1isassociatedwithtff2methylationingastriccancer
AT wangmeilin muc1isassociatedwithtff2methylationingastriccancer
AT chuhaiyan muc1isassociatedwithtff2methylationingastriccancer
AT zhanghaiyan muc1isassociatedwithtff2methylationingastriccancer
AT zhangzhengdong muc1isassociatedwithtff2methylationingastriccancer