Cargando…

Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin

BACKGROUND AND PURPOSE: In the present study, we tried for the first time to examine whether cinnamaldehyde (CA), with herbal nature, can be co-administrated with doxorubicin (DOX, as an anticancer drug) toward U87MG glioblastoma cells to potentiate its cytotoxic effect and overcome or reduce its si...

Descripción completa

Detalles Bibliográficos
Autores principales: Abbasi, Abbas, Hajialyani, Marziyeh, Hosseinzadeh, Leila, Jalilian, Fereshteh, Yaghmaei, Parichehr, Jamshidi Navid, Sahar, Motamed, Hajar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053293/
https://www.ncbi.nlm.nih.gov/pubmed/32180814
http://dx.doi.org/10.4103/1735-5362.278712
_version_ 1783503012680957952
author Abbasi, Abbas
Hajialyani, Marziyeh
Hosseinzadeh, Leila
Jalilian, Fereshteh
Yaghmaei, Parichehr
Jamshidi Navid, Sahar
Motamed, Hajar
author_facet Abbasi, Abbas
Hajialyani, Marziyeh
Hosseinzadeh, Leila
Jalilian, Fereshteh
Yaghmaei, Parichehr
Jamshidi Navid, Sahar
Motamed, Hajar
author_sort Abbasi, Abbas
collection PubMed
description BACKGROUND AND PURPOSE: In the present study, we tried for the first time to examine whether cinnamaldehyde (CA), with herbal nature, can be co-administrated with doxorubicin (DOX, as an anticancer drug) toward U87MG glioblastoma cells to potentiate its cytotoxic effect and overcome or reduce its side effects. EXPERIMENTAL APPROACH: The cytotoxic effect of DOX and CA, either individually or in combination, were evaluated on U87MG cells using the MTT method. The mechanism of action was studied by investigating the mode of cell death using caspase-3 and 9 activations, mitochondrial membrane potential (MMP) as well as sub G1 analysis. The expression of apoptosis- related genes (Bcl-2 and Bax) was also examined. FINDINGS / RESULTS: Cellular toxicity assay revealed that CA and DOX can potentially reduce the viability of U87MG cells with IC50 at 11.6 and 5 μg/mL, respectively. Exposure with the combination of CA and DOX significantly increased cytotoxic effect of DOX on U87MG cells. The results of SUBG1, MMP, and also caspase-3 and -9 activity assays, in association with the results corresponding to the Bax and Bcl-2 gene expressions, altogether revealed that CA can induce apoptosis on U87MG cells. Moreover, apoptogenic effects of DOX were found to be potentiated by CA. CONCLUSION AND IMPLICATIONS: The results of this study revealed the promising cytotoxic and apoptogenic role of CA on U87MG cells. Additionally, our findings demonstrated that CA is able to enhance the apoptosis induced by DOX on human glioblastoma cells. Collectively, these data suggested that co-exposure of CA and DOX could be effective for treatment of glioblastoma, but further in vivo and clinical studies are still needed to prove these results.
format Online
Article
Text
id pubmed-7053293
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-70532932020-03-16 Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin Abbasi, Abbas Hajialyani, Marziyeh Hosseinzadeh, Leila Jalilian, Fereshteh Yaghmaei, Parichehr Jamshidi Navid, Sahar Motamed, Hajar Res Pharm Sci Original Article BACKGROUND AND PURPOSE: In the present study, we tried for the first time to examine whether cinnamaldehyde (CA), with herbal nature, can be co-administrated with doxorubicin (DOX, as an anticancer drug) toward U87MG glioblastoma cells to potentiate its cytotoxic effect and overcome or reduce its side effects. EXPERIMENTAL APPROACH: The cytotoxic effect of DOX and CA, either individually or in combination, were evaluated on U87MG cells using the MTT method. The mechanism of action was studied by investigating the mode of cell death using caspase-3 and 9 activations, mitochondrial membrane potential (MMP) as well as sub G1 analysis. The expression of apoptosis- related genes (Bcl-2 and Bax) was also examined. FINDINGS / RESULTS: Cellular toxicity assay revealed that CA and DOX can potentially reduce the viability of U87MG cells with IC50 at 11.6 and 5 μg/mL, respectively. Exposure with the combination of CA and DOX significantly increased cytotoxic effect of DOX on U87MG cells. The results of SUBG1, MMP, and also caspase-3 and -9 activity assays, in association with the results corresponding to the Bax and Bcl-2 gene expressions, altogether revealed that CA can induce apoptosis on U87MG cells. Moreover, apoptogenic effects of DOX were found to be potentiated by CA. CONCLUSION AND IMPLICATIONS: The results of this study revealed the promising cytotoxic and apoptogenic role of CA on U87MG cells. Additionally, our findings demonstrated that CA is able to enhance the apoptosis induced by DOX on human glioblastoma cells. Collectively, these data suggested that co-exposure of CA and DOX could be effective for treatment of glioblastoma, but further in vivo and clinical studies are still needed to prove these results. Wolters Kluwer - Medknow 2020-02-20 /pmc/articles/PMC7053293/ /pubmed/32180814 http://dx.doi.org/10.4103/1735-5362.278712 Text en Copyright: © 2020 Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Abbasi, Abbas
Hajialyani, Marziyeh
Hosseinzadeh, Leila
Jalilian, Fereshteh
Yaghmaei, Parichehr
Jamshidi Navid, Sahar
Motamed, Hajar
Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin
title Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin
title_full Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin
title_fullStr Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin
title_full_unstemmed Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin
title_short Evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on U87MG cells alone and in combination with doxorubicin
title_sort evaluation of the cytotoxic and apoptogenic effects of cinnamaldehyde on u87mg cells alone and in combination with doxorubicin
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053293/
https://www.ncbi.nlm.nih.gov/pubmed/32180814
http://dx.doi.org/10.4103/1735-5362.278712
work_keys_str_mv AT abbasiabbas evaluationofthecytotoxicandapoptogeniceffectsofcinnamaldehydeonu87mgcellsaloneandincombinationwithdoxorubicin
AT hajialyanimarziyeh evaluationofthecytotoxicandapoptogeniceffectsofcinnamaldehydeonu87mgcellsaloneandincombinationwithdoxorubicin
AT hosseinzadehleila evaluationofthecytotoxicandapoptogeniceffectsofcinnamaldehydeonu87mgcellsaloneandincombinationwithdoxorubicin
AT jalilianfereshteh evaluationofthecytotoxicandapoptogeniceffectsofcinnamaldehydeonu87mgcellsaloneandincombinationwithdoxorubicin
AT yaghmaeiparichehr evaluationofthecytotoxicandapoptogeniceffectsofcinnamaldehydeonu87mgcellsaloneandincombinationwithdoxorubicin
AT jamshidinavidsahar evaluationofthecytotoxicandapoptogeniceffectsofcinnamaldehydeonu87mgcellsaloneandincombinationwithdoxorubicin
AT motamedhajar evaluationofthecytotoxicandapoptogeniceffectsofcinnamaldehydeonu87mgcellsaloneandincombinationwithdoxorubicin