Cargando…
Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis
Amyotrophic lateral sclerosis (ALS) is a serious disease characterized by the degeneration of motor neurons resulting in muscle weakness and paralysis. The neuroendocrine polypeptide VGF is localized in the central nervous system and peripheral endocrine neurons and is cleaved into several polypepti...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053308/ https://www.ncbi.nlm.nih.gov/pubmed/32174778 http://dx.doi.org/10.7150/ijms.39101 |
_version_ | 1783503015114702848 |
---|---|
author | Noda, Yasuhiro Tanaka, Miruto Nakamura, Shinsuke Ito, Junko Kakita, Akiyoshi Hara, Hideaki Shimazawa, Masamitsu |
author_facet | Noda, Yasuhiro Tanaka, Miruto Nakamura, Shinsuke Ito, Junko Kakita, Akiyoshi Hara, Hideaki Shimazawa, Masamitsu |
author_sort | Noda, Yasuhiro |
collection | PubMed |
description | Amyotrophic lateral sclerosis (ALS) is a serious disease characterized by the degeneration of motor neurons resulting in muscle weakness and paralysis. The neuroendocrine polypeptide VGF is localized in the central nervous system and peripheral endocrine neurons and is cleaved into several polypeptides with multiple functions. Previous studies revealed that VGF was decreased in the cerebrospinal fluid of ALS model mice and sporadic ALS patients. However, it is unknown which cells supply VGF in the spinal cord and a detailed localization is lacking. In this study, we evaluated the VGF-producing cells and protein localization using in situ hybridization and immunostaining in the spinal cords of ALS and control patients. VGF mRNA was localized both in the dorsal and anterior horns of the spinal cords. Moreover, in the anterior horn, VGF mRNA co-localized with a neurofilament heavy chain, which is a motor neuron marker, and VGF mRNA-positive motor neurons were decreased in the spinal cords of ALS patients. We revealed that VGF protein level was decreased in the anterior horn of ALS patients; however, the expression level of VGF protein was not changed in the posterior horn or white matter. Furthermore, the expression level of VGF protein was conserved in ALS patients with long-term survival. These results reveal that VGF is mainly supplied by human motor neurons, and suggest that VGF expression changes may be involved in ALS pathology. |
format | Online Article Text |
id | pubmed-7053308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-70533082020-03-13 Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis Noda, Yasuhiro Tanaka, Miruto Nakamura, Shinsuke Ito, Junko Kakita, Akiyoshi Hara, Hideaki Shimazawa, Masamitsu Int J Med Sci Research Paper Amyotrophic lateral sclerosis (ALS) is a serious disease characterized by the degeneration of motor neurons resulting in muscle weakness and paralysis. The neuroendocrine polypeptide VGF is localized in the central nervous system and peripheral endocrine neurons and is cleaved into several polypeptides with multiple functions. Previous studies revealed that VGF was decreased in the cerebrospinal fluid of ALS model mice and sporadic ALS patients. However, it is unknown which cells supply VGF in the spinal cord and a detailed localization is lacking. In this study, we evaluated the VGF-producing cells and protein localization using in situ hybridization and immunostaining in the spinal cords of ALS and control patients. VGF mRNA was localized both in the dorsal and anterior horns of the spinal cords. Moreover, in the anterior horn, VGF mRNA co-localized with a neurofilament heavy chain, which is a motor neuron marker, and VGF mRNA-positive motor neurons were decreased in the spinal cords of ALS patients. We revealed that VGF protein level was decreased in the anterior horn of ALS patients; however, the expression level of VGF protein was not changed in the posterior horn or white matter. Furthermore, the expression level of VGF protein was conserved in ALS patients with long-term survival. These results reveal that VGF is mainly supplied by human motor neurons, and suggest that VGF expression changes may be involved in ALS pathology. Ivyspring International Publisher 2020-02-04 /pmc/articles/PMC7053308/ /pubmed/32174778 http://dx.doi.org/10.7150/ijms.39101 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Noda, Yasuhiro Tanaka, Miruto Nakamura, Shinsuke Ito, Junko Kakita, Akiyoshi Hara, Hideaki Shimazawa, Masamitsu Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis |
title | Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis |
title_full | Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis |
title_fullStr | Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis |
title_full_unstemmed | Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis |
title_short | Identification of VGF nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis |
title_sort | identification of vgf nerve growth factor inducible-producing cells in human spinal cords and expression change in patients with amyotrophic lateral sclerosis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053308/ https://www.ncbi.nlm.nih.gov/pubmed/32174778 http://dx.doi.org/10.7150/ijms.39101 |
work_keys_str_mv | AT nodayasuhiro identificationofvgfnervegrowthfactorinducibleproducingcellsinhumanspinalcordsandexpressionchangeinpatientswithamyotrophiclateralsclerosis AT tanakamiruto identificationofvgfnervegrowthfactorinducibleproducingcellsinhumanspinalcordsandexpressionchangeinpatientswithamyotrophiclateralsclerosis AT nakamurashinsuke identificationofvgfnervegrowthfactorinducibleproducingcellsinhumanspinalcordsandexpressionchangeinpatientswithamyotrophiclateralsclerosis AT itojunko identificationofvgfnervegrowthfactorinducibleproducingcellsinhumanspinalcordsandexpressionchangeinpatientswithamyotrophiclateralsclerosis AT kakitaakiyoshi identificationofvgfnervegrowthfactorinducibleproducingcellsinhumanspinalcordsandexpressionchangeinpatientswithamyotrophiclateralsclerosis AT harahideaki identificationofvgfnervegrowthfactorinducibleproducingcellsinhumanspinalcordsandexpressionchangeinpatientswithamyotrophiclateralsclerosis AT shimazawamasamitsu identificationofvgfnervegrowthfactorinducibleproducingcellsinhumanspinalcordsandexpressionchangeinpatientswithamyotrophiclateralsclerosis |