Humanin gene expression in fibroblast of Down syndrome subjects

Down syndrome (DS) is characterized by trisomy of chromosome 21 and peculiar phenotype. Humanin (HN) is a mitochondrial short 24-residue polypeptide whit anti-apoptotic and neuroprotective effects. In this study we evaluated HN protein expression and HN mRNA levels in cultured fibroblasts from DS pa...

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Autores principales: Salemi, Michele, Ridolfo, Federico, Salluzzo, Maria Grazia, Cannarrella, Rossella, Giambirtone, Mariaconcetta, Caniglia, Salvatore, Tirolo, Cataldo, Ferri, Raffaele, Romano, Corrado
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053358/
https://www.ncbi.nlm.nih.gov/pubmed/32132866
http://dx.doi.org/10.7150/ijms.39145
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author Salemi, Michele
Ridolfo, Federico
Salluzzo, Maria Grazia
Cannarrella, Rossella
Giambirtone, Mariaconcetta
Caniglia, Salvatore
Tirolo, Cataldo
Ferri, Raffaele
Romano, Corrado
author_facet Salemi, Michele
Ridolfo, Federico
Salluzzo, Maria Grazia
Cannarrella, Rossella
Giambirtone, Mariaconcetta
Caniglia, Salvatore
Tirolo, Cataldo
Ferri, Raffaele
Romano, Corrado
author_sort Salemi, Michele
collection PubMed
description Down syndrome (DS) is characterized by trisomy of chromosome 21 and peculiar phenotype. Humanin (HN) is a mitochondrial short 24-residue polypeptide whit anti-apoptotic and neuroprotective effects. In this study we evaluated HN protein expression and HN mRNA levels in cultured fibroblasts from DS patients and normal controls. Our results obtained by immunocytochemistry, western-blot and qRT-PCR analysis show a significant HN up-regulation in DS patients. These results confirm previous studies and suggest a role for HN may in the DS phenotype.
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spelling pubmed-70533582020-03-04 Humanin gene expression in fibroblast of Down syndrome subjects Salemi, Michele Ridolfo, Federico Salluzzo, Maria Grazia Cannarrella, Rossella Giambirtone, Mariaconcetta Caniglia, Salvatore Tirolo, Cataldo Ferri, Raffaele Romano, Corrado Int J Med Sci Research Paper Down syndrome (DS) is characterized by trisomy of chromosome 21 and peculiar phenotype. Humanin (HN) is a mitochondrial short 24-residue polypeptide whit anti-apoptotic and neuroprotective effects. In this study we evaluated HN protein expression and HN mRNA levels in cultured fibroblasts from DS patients and normal controls. Our results obtained by immunocytochemistry, western-blot and qRT-PCR analysis show a significant HN up-regulation in DS patients. These results confirm previous studies and suggest a role for HN may in the DS phenotype. Ivyspring International Publisher 2020-01-18 /pmc/articles/PMC7053358/ /pubmed/32132866 http://dx.doi.org/10.7150/ijms.39145 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Salemi, Michele
Ridolfo, Federico
Salluzzo, Maria Grazia
Cannarrella, Rossella
Giambirtone, Mariaconcetta
Caniglia, Salvatore
Tirolo, Cataldo
Ferri, Raffaele
Romano, Corrado
Humanin gene expression in fibroblast of Down syndrome subjects
title Humanin gene expression in fibroblast of Down syndrome subjects
title_full Humanin gene expression in fibroblast of Down syndrome subjects
title_fullStr Humanin gene expression in fibroblast of Down syndrome subjects
title_full_unstemmed Humanin gene expression in fibroblast of Down syndrome subjects
title_short Humanin gene expression in fibroblast of Down syndrome subjects
title_sort humanin gene expression in fibroblast of down syndrome subjects
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053358/
https://www.ncbi.nlm.nih.gov/pubmed/32132866
http://dx.doi.org/10.7150/ijms.39145
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