Cargando…
Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression
OBJECTIVE: In renal ischemia/reperfusion injury (RIRI), nuclear factor κB (NF-κB (NF-κB (NF- METHODS: Eighteen male Sprague-Dawley rats were randomly allocated into the sham group, the I/R group, and the VNS+I/R group, 6 rats per group. An RIRI model was induced by a right nephrectomy and blockade o...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053466/ https://www.ncbi.nlm.nih.gov/pubmed/32148655 http://dx.doi.org/10.1155/2020/7106525 |
_version_ | 1783503046229098496 |
---|---|
author | Wang, Meng Deng, Jielin Lai, Huanzhu Lai, Yanqiu Meng, Guannan Wang, Zhenya Zhou, Zhen Chen, Hu Yu, Zhiyao Li, Shuyan Jiang, Hong |
author_facet | Wang, Meng Deng, Jielin Lai, Huanzhu Lai, Yanqiu Meng, Guannan Wang, Zhenya Zhou, Zhen Chen, Hu Yu, Zhiyao Li, Shuyan Jiang, Hong |
author_sort | Wang, Meng |
collection | PubMed |
description | OBJECTIVE: In renal ischemia/reperfusion injury (RIRI), nuclear factor κB (NF-κB (NF-κB (NF- METHODS: Eighteen male Sprague-Dawley rats were randomly allocated into the sham group, the I/R group, and the VNS+I/R group, 6 rats per group. An RIRI model was induced by a right nephrectomy and blockade of the left renal pedicle vessels for 45 min. After 6 h of reperfusion, the blood samples and renal samples were collected. The VNS treatment was performed throughout the I/R process in the VNS+I/R group using specific parameters (20 Hz, 0.1 ms in duration, square waves) known to produce a small but reliable bradycardia. Blood was used for evaluation of renal function and inflammatory state. Renal injury was evaluated via TUNEL staining. Renal samples were harvested to evaluate renal oxidative stress, NF-κB (NF- RESULTS: The VNS treatment reduces serum creatinine (Cr) and blood urea nitrogen (BUN) levels. Simultaneously, the levels of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1-beta (IL-1β) were significantly increased in the I/R group, but VNS treatment markedly ameliorated this inflammatory response. Furthermore, the VNS ameliorated oxidant stress and renal injury, indicated by a decrease in 3-nitrotyrosine (3-NT) formation and MDA and MPO levels and an increase in the SOD level compared to that in the I/R group. Finally, the VNS also significantly decreases NF-κB (NF- CONCLUSION: Our findings indicate that NF-κB activation increased iNOS expression and promoted RIRI and that VNS treatment attenuated RIRI by inhibiting iNOS expression, oxidative stress, and inflammation via NF-κB inactivation.κB (NF-κB (NF- |
format | Online Article Text |
id | pubmed-7053466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-70534662020-03-08 Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression Wang, Meng Deng, Jielin Lai, Huanzhu Lai, Yanqiu Meng, Guannan Wang, Zhenya Zhou, Zhen Chen, Hu Yu, Zhiyao Li, Shuyan Jiang, Hong Oxid Med Cell Longev Research Article OBJECTIVE: In renal ischemia/reperfusion injury (RIRI), nuclear factor κB (NF-κB (NF-κB (NF- METHODS: Eighteen male Sprague-Dawley rats were randomly allocated into the sham group, the I/R group, and the VNS+I/R group, 6 rats per group. An RIRI model was induced by a right nephrectomy and blockade of the left renal pedicle vessels for 45 min. After 6 h of reperfusion, the blood samples and renal samples were collected. The VNS treatment was performed throughout the I/R process in the VNS+I/R group using specific parameters (20 Hz, 0.1 ms in duration, square waves) known to produce a small but reliable bradycardia. Blood was used for evaluation of renal function and inflammatory state. Renal injury was evaluated via TUNEL staining. Renal samples were harvested to evaluate renal oxidative stress, NF-κB (NF- RESULTS: The VNS treatment reduces serum creatinine (Cr) and blood urea nitrogen (BUN) levels. Simultaneously, the levels of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1-beta (IL-1β) were significantly increased in the I/R group, but VNS treatment markedly ameliorated this inflammatory response. Furthermore, the VNS ameliorated oxidant stress and renal injury, indicated by a decrease in 3-nitrotyrosine (3-NT) formation and MDA and MPO levels and an increase in the SOD level compared to that in the I/R group. Finally, the VNS also significantly decreases NF-κB (NF- CONCLUSION: Our findings indicate that NF-κB activation increased iNOS expression and promoted RIRI and that VNS treatment attenuated RIRI by inhibiting iNOS expression, oxidative stress, and inflammation via NF-κB inactivation.κB (NF-κB (NF- Hindawi 2020-02-20 /pmc/articles/PMC7053466/ /pubmed/32148655 http://dx.doi.org/10.1155/2020/7106525 Text en Copyright © 2020 Meng Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Meng Deng, Jielin Lai, Huanzhu Lai, Yanqiu Meng, Guannan Wang, Zhenya Zhou, Zhen Chen, Hu Yu, Zhiyao Li, Shuyan Jiang, Hong Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression |
title | Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression |
title_full | Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression |
title_fullStr | Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression |
title_full_unstemmed | Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression |
title_short | Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression |
title_sort | vagus nerve stimulation ameliorates renal ischemia-reperfusion injury through inhibiting nf-κb activation and inos protein expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053466/ https://www.ncbi.nlm.nih.gov/pubmed/32148655 http://dx.doi.org/10.1155/2020/7106525 |
work_keys_str_mv | AT wangmeng vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT dengjielin vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT laihuanzhu vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT laiyanqiu vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT mengguannan vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT wangzhenya vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT zhouzhen vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT chenhu vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT yuzhiyao vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT lishuyan vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression AT jianghong vagusnervestimulationamelioratesrenalischemiareperfusioninjurythroughinhibitingnfkbactivationandinosproteinexpression |