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Genetic variant of SPARC gene and its association with growth traits in Chinese cattle
SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related resea...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Copernicus GmbH
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053509/ https://www.ncbi.nlm.nih.gov/pubmed/32166110 http://dx.doi.org/10.5194/aab-63-31-2020 |
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author | Zhang, Danyang Xu, Jiawei Yang, Peng Wen, Yifan He, Hua Li, Jiaxiao Liang, Juntong Zheng, Yining Zhang, Zijing Wang, Xianwei Yu, Xiang Wang, Eryao Lei, Chuzhao Chen, Hong Huang, Yongzhen |
author_facet | Zhang, Danyang Xu, Jiawei Yang, Peng Wen, Yifan He, Hua Li, Jiaxiao Liang, Juntong Zheng, Yining Zhang, Zijing Wang, Xianwei Yu, Xiang Wang, Eryao Lei, Chuzhao Chen, Hong Huang, Yongzhen |
author_sort | Zhang, Danyang |
collection | PubMed |
description | SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related research. Especially the muscle and fat metabolism are closely related. In this study, we used a DNA pool to detect a new SNP site (g.12454T [Formula: see text] C). A total of 616 samples of four breeds of Qinchuan cattle (QC, [Formula: see text]), Xianan cattle (XN, [Formula: see text]), Pinan cattle (PN, [Formula: see text]) and Jiaxian cattle (JX, [Formula: see text]) were analyzed and identified with ARMS-PCR. In addition, we correlated SNP with growth traits and showed significant correlation with growth traits such as rump length, hip width, and body length ([Formula: see text]). Moreover, we tested the SPARC gene expression level in different tissues belonging to XN adult cattle ([Formula: see text]) and found its high expression in muscle tissues (relative to the kidney). Further, we found the SNP is able to increase the SPARC expression level in skeletal muscle ([Formula: see text]). According to statistical data, this SNP site may be applied to a molecular marker of an early marker-assisted selection for early growth of beef cattle. |
format | Online Article Text |
id | pubmed-7053509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Copernicus GmbH |
record_format | MEDLINE/PubMed |
spelling | pubmed-70535092020-03-12 Genetic variant of SPARC gene and its association with growth traits in Chinese cattle Zhang, Danyang Xu, Jiawei Yang, Peng Wen, Yifan He, Hua Li, Jiaxiao Liang, Juntong Zheng, Yining Zhang, Zijing Wang, Xianwei Yu, Xiang Wang, Eryao Lei, Chuzhao Chen, Hong Huang, Yongzhen Arch Anim Breed Original Study SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related research. Especially the muscle and fat metabolism are closely related. In this study, we used a DNA pool to detect a new SNP site (g.12454T [Formula: see text] C). A total of 616 samples of four breeds of Qinchuan cattle (QC, [Formula: see text]), Xianan cattle (XN, [Formula: see text]), Pinan cattle (PN, [Formula: see text]) and Jiaxian cattle (JX, [Formula: see text]) were analyzed and identified with ARMS-PCR. In addition, we correlated SNP with growth traits and showed significant correlation with growth traits such as rump length, hip width, and body length ([Formula: see text]). Moreover, we tested the SPARC gene expression level in different tissues belonging to XN adult cattle ([Formula: see text]) and found its high expression in muscle tissues (relative to the kidney). Further, we found the SNP is able to increase the SPARC expression level in skeletal muscle ([Formula: see text]). According to statistical data, this SNP site may be applied to a molecular marker of an early marker-assisted selection for early growth of beef cattle. Copernicus GmbH 2020-01-30 /pmc/articles/PMC7053509/ /pubmed/32166110 http://dx.doi.org/10.5194/aab-63-31-2020 Text en Copyright: © 2020 Danyang Zhang et al. This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Original Study Zhang, Danyang Xu, Jiawei Yang, Peng Wen, Yifan He, Hua Li, Jiaxiao Liang, Juntong Zheng, Yining Zhang, Zijing Wang, Xianwei Yu, Xiang Wang, Eryao Lei, Chuzhao Chen, Hong Huang, Yongzhen Genetic variant of SPARC gene and its association with growth traits in Chinese cattle |
title | Genetic variant of SPARC gene and its association with growth traits in Chinese cattle |
title_full | Genetic variant of SPARC gene and its association with growth traits in Chinese cattle |
title_fullStr | Genetic variant of SPARC gene and its association with growth traits in Chinese cattle |
title_full_unstemmed | Genetic variant of SPARC gene and its association with growth traits in Chinese cattle |
title_short | Genetic variant of SPARC gene and its association with growth traits in Chinese cattle |
title_sort | genetic variant of sparc gene and its association with growth traits in chinese cattle |
topic | Original Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053509/ https://www.ncbi.nlm.nih.gov/pubmed/32166110 http://dx.doi.org/10.5194/aab-63-31-2020 |
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