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ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma
Clear cell renal cell carcinoma (ccRCC) is one of the most common malignancies and lacks reliable biomarkers for diagnosis and prognosis, which results in high incidence and mortality rates of ccRCC. In this study, ISG20, HJURP, and FOXM1 were identified as hub genes via weighted gene co-expression...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053611/ https://www.ncbi.nlm.nih.gov/pubmed/32003757 http://dx.doi.org/10.18632/aging.102714 |
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author | Xu, Tianbo Ruan, Hailong Gao, Su Liu, Jingchong Liu, Yuenan Song, Zhengshuai Cao, Qi Wang, Keshan Bao, Lin Liu, Di Tong, Junwei Shi, Jian Liang, Huageng Yang, Hongmei Chen, Ke Zhang, Xiaoping |
author_facet | Xu, Tianbo Ruan, Hailong Gao, Su Liu, Jingchong Liu, Yuenan Song, Zhengshuai Cao, Qi Wang, Keshan Bao, Lin Liu, Di Tong, Junwei Shi, Jian Liang, Huageng Yang, Hongmei Chen, Ke Zhang, Xiaoping |
author_sort | Xu, Tianbo |
collection | PubMed |
description | Clear cell renal cell carcinoma (ccRCC) is one of the most common malignancies and lacks reliable biomarkers for diagnosis and prognosis, which results in high incidence and mortality rates of ccRCC. In this study, ISG20, HJURP, and FOXM1 were identified as hub genes via weighted gene co-expression network analysis (WGCNA) and Cox regression analysis. Samples validation showed that only ISG20 was up-regulated in ccRCC. Therefore, ISG20 was selected for further study. High ISG20 expression was associated with poor overall survival and disease-free survival. Furthermore, the expression of ISG20 could effectively differentiate ccRCC from normal tissues and was positively correlated to clinical stages. Functional experiments proved that knockdown of ISG20 expression could obviously inhibit cell growth, migration, and invasion in ccRCC cells. To find the potential mechanisms of ISG20, gene set enrichment analysis (GSEA) was performed and revealed that high expression of ISG20 was significantly involved in metastasis and cell cycle pathways. In addition, we found that ISG20 could regulate the expression of MMP9 and CCND1. In conclusion, these findings suggested that ISG20 promoted cell proliferation and metastasis via regulating MMP9/CCND1 expression and might serve as a potential biomarker and therapeutic target in ccRCC. |
format | Online Article Text |
id | pubmed-7053611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-70536112020-03-12 ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma Xu, Tianbo Ruan, Hailong Gao, Su Liu, Jingchong Liu, Yuenan Song, Zhengshuai Cao, Qi Wang, Keshan Bao, Lin Liu, Di Tong, Junwei Shi, Jian Liang, Huageng Yang, Hongmei Chen, Ke Zhang, Xiaoping Aging (Albany NY) Research Paper Clear cell renal cell carcinoma (ccRCC) is one of the most common malignancies and lacks reliable biomarkers for diagnosis and prognosis, which results in high incidence and mortality rates of ccRCC. In this study, ISG20, HJURP, and FOXM1 were identified as hub genes via weighted gene co-expression network analysis (WGCNA) and Cox regression analysis. Samples validation showed that only ISG20 was up-regulated in ccRCC. Therefore, ISG20 was selected for further study. High ISG20 expression was associated with poor overall survival and disease-free survival. Furthermore, the expression of ISG20 could effectively differentiate ccRCC from normal tissues and was positively correlated to clinical stages. Functional experiments proved that knockdown of ISG20 expression could obviously inhibit cell growth, migration, and invasion in ccRCC cells. To find the potential mechanisms of ISG20, gene set enrichment analysis (GSEA) was performed and revealed that high expression of ISG20 was significantly involved in metastasis and cell cycle pathways. In addition, we found that ISG20 could regulate the expression of MMP9 and CCND1. In conclusion, these findings suggested that ISG20 promoted cell proliferation and metastasis via regulating MMP9/CCND1 expression and might serve as a potential biomarker and therapeutic target in ccRCC. Impact Journals 2020-01-30 /pmc/articles/PMC7053611/ /pubmed/32003757 http://dx.doi.org/10.18632/aging.102714 Text en Copyright © 2020 Xu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xu, Tianbo Ruan, Hailong Gao, Su Liu, Jingchong Liu, Yuenan Song, Zhengshuai Cao, Qi Wang, Keshan Bao, Lin Liu, Di Tong, Junwei Shi, Jian Liang, Huageng Yang, Hongmei Chen, Ke Zhang, Xiaoping ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma |
title | ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma |
title_full | ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma |
title_fullStr | ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma |
title_full_unstemmed | ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma |
title_short | ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma |
title_sort | isg20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053611/ https://www.ncbi.nlm.nih.gov/pubmed/32003757 http://dx.doi.org/10.18632/aging.102714 |
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