Cargando…

LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis

PURPOSE: Glioma is an aggressive tumor from the nervous system, which causes more than 70% of primary malignant brain tumors. Considering its severe malignancy, there is an urgent need to investigate more practical markers to understand the pathogenesis of glioma, and potential treatment methods for...

Descripción completa

Detalles Bibliográficos
Autores principales: Xin, Yanchao, Zhang, Wuzhong, Mao, Chongchong, Li, Jianxin, Liu, Xianzhi, Zhao, Junbo, Xue, Junfeng, Li, Junqing, Ren, Yonglu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053821/
https://www.ncbi.nlm.nih.gov/pubmed/32184618
http://dx.doi.org/10.2147/OTT.S230895
_version_ 1783503114760880128
author Xin, Yanchao
Zhang, Wuzhong
Mao, Chongchong
Li, Jianxin
Liu, Xianzhi
Zhao, Junbo
Xue, Junfeng
Li, Junqing
Ren, Yonglu
author_facet Xin, Yanchao
Zhang, Wuzhong
Mao, Chongchong
Li, Jianxin
Liu, Xianzhi
Zhao, Junbo
Xue, Junfeng
Li, Junqing
Ren, Yonglu
author_sort Xin, Yanchao
collection PubMed
description PURPOSE: Glioma is an aggressive tumor from the nervous system, which causes more than 70% of primary malignant brain tumors. Considering its severe malignancy, there is an urgent need to investigate more practical markers to understand the pathogenesis of glioma, and potential treatment methods for glioma patients. In the paper, we are focused on examining the roles of LINC01140, miR-199a-3p, and ZHX1 in the progression of gliomas, as well as their inner associations and modulation mechanisms. METHODS: qRT-PCR was employed to examine the expression levels of LINC01140 and miR-199a-3p. We measured the expressions of ZHX1 via qRT-PCR and Western blotting. CCK8 assays, migration assays, and invasion assays were carried out to determine the cell viabilities and abilities of migration and invasion. We also conducted in vivo tumor growth experiments to investigate the roles of LINC01140 in glioma developments. RESULTS: The expressions of LINC01140 were promoted in glioma. Silencing LINC01140 could inhibit glioma cell viabilities, migration, and invasion. In our experiments, miR-199a-3p was inhibited in glioma. LINC01140 negatively regulated the expressions of miR-199a-3p in glioma. MiR-199a-3p could target ZHX1 to inhibit its expression in glioma cells. CONCLUSION: LINC01140 could promote glioma developments by modulating the miR-199a-3p/ZHX1 axis.
format Online
Article
Text
id pubmed-7053821
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-70538212020-03-17 LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis Xin, Yanchao Zhang, Wuzhong Mao, Chongchong Li, Jianxin Liu, Xianzhi Zhao, Junbo Xue, Junfeng Li, Junqing Ren, Yonglu Onco Targets Ther Original Research PURPOSE: Glioma is an aggressive tumor from the nervous system, which causes more than 70% of primary malignant brain tumors. Considering its severe malignancy, there is an urgent need to investigate more practical markers to understand the pathogenesis of glioma, and potential treatment methods for glioma patients. In the paper, we are focused on examining the roles of LINC01140, miR-199a-3p, and ZHX1 in the progression of gliomas, as well as their inner associations and modulation mechanisms. METHODS: qRT-PCR was employed to examine the expression levels of LINC01140 and miR-199a-3p. We measured the expressions of ZHX1 via qRT-PCR and Western blotting. CCK8 assays, migration assays, and invasion assays were carried out to determine the cell viabilities and abilities of migration and invasion. We also conducted in vivo tumor growth experiments to investigate the roles of LINC01140 in glioma developments. RESULTS: The expressions of LINC01140 were promoted in glioma. Silencing LINC01140 could inhibit glioma cell viabilities, migration, and invasion. In our experiments, miR-199a-3p was inhibited in glioma. LINC01140 negatively regulated the expressions of miR-199a-3p in glioma. MiR-199a-3p could target ZHX1 to inhibit its expression in glioma cells. CONCLUSION: LINC01140 could promote glioma developments by modulating the miR-199a-3p/ZHX1 axis. Dove 2020-02-28 /pmc/articles/PMC7053821/ /pubmed/32184618 http://dx.doi.org/10.2147/OTT.S230895 Text en © 2020 Xin et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Xin, Yanchao
Zhang, Wuzhong
Mao, Chongchong
Li, Jianxin
Liu, Xianzhi
Zhao, Junbo
Xue, Junfeng
Li, Junqing
Ren, Yonglu
LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis
title LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis
title_full LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis
title_fullStr LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis
title_full_unstemmed LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis
title_short LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis
title_sort lncrna linc01140 inhibits glioma cell migration and invasion via modulation of mir-199a-3p/zhx1 axis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053821/
https://www.ncbi.nlm.nih.gov/pubmed/32184618
http://dx.doi.org/10.2147/OTT.S230895
work_keys_str_mv AT xinyanchao lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis
AT zhangwuzhong lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis
AT maochongchong lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis
AT lijianxin lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis
AT liuxianzhi lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis
AT zhaojunbo lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis
AT xuejunfeng lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis
AT lijunqing lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis
AT renyonglu lncrnalinc01140inhibitsgliomacellmigrationandinvasionviamodulationofmir199a3pzhx1axis