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Neural maturation enhanced by exercise-induced extracellular derivatives
Physical activity has profound effects on neuronal progenitor cell growth, differentiation, and integration, but the mechanism for these effects is still ambiguous. Using a mouse model, we investigated the effects of two weeks of treadmill running on the dynamics of the size distribution and miRNA p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054262/ https://www.ncbi.nlm.nih.gov/pubmed/32127592 http://dx.doi.org/10.1038/s41598-020-60930-6 |
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author | Moon, Hyo Youl Yoon, Kyeong Jin Lee, Won Sang Cho, Hae-Sung Kim, Do-Yeon Kim, Ji-Seok |
author_facet | Moon, Hyo Youl Yoon, Kyeong Jin Lee, Won Sang Cho, Hae-Sung Kim, Do-Yeon Kim, Ji-Seok |
author_sort | Moon, Hyo Youl |
collection | PubMed |
description | Physical activity has profound effects on neuronal progenitor cell growth, differentiation, and integration, but the mechanism for these effects is still ambiguous. Using a mouse model, we investigated the effects of two weeks of treadmill running on the dynamics of the size distribution and miRNA profiles of serum extracellular derivatives (EDs) using particle-sizing analysis and small RNA sequencing. We found that an increased average diameter of EDs in the running group compared with the sedentary group (p < 0.05), and 16 miRNAs were significantly altered (p < 0.05) in the running group. Furthermore, functional annotation analysis of differentially expressed miRNA-predicted target genes showed that many of these target genes are involved in the PI3K-Akt pathway. Exercise-induced serum EDs increased Neuro2A cell viability and Akt phosphorylation. We also found that expression levels of neuronal maturation markers such as Microtubule-Associated Protein 2 (MAP2ab) and Neuronal nuclei (NeuN) were increased (p < 0.05, respectively), and that inhibition of the PI3K-Akt pathway by LY294002 pre-treatment ameliorated their expression in Neuro2A cells. Finally, the administration of exercise-induced EDs for 3 days increased the Histone 3 phosphorylation and β-III tubulin expression in Ink/Arf null neural stem cells and progenitors (NSPCs) under each proliferation and differentiation condition. These results suggest that exercise-induced circulating EDs may mediate neuronal maturation during exercise. |
format | Online Article Text |
id | pubmed-7054262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70542622020-03-11 Neural maturation enhanced by exercise-induced extracellular derivatives Moon, Hyo Youl Yoon, Kyeong Jin Lee, Won Sang Cho, Hae-Sung Kim, Do-Yeon Kim, Ji-Seok Sci Rep Article Physical activity has profound effects on neuronal progenitor cell growth, differentiation, and integration, but the mechanism for these effects is still ambiguous. Using a mouse model, we investigated the effects of two weeks of treadmill running on the dynamics of the size distribution and miRNA profiles of serum extracellular derivatives (EDs) using particle-sizing analysis and small RNA sequencing. We found that an increased average diameter of EDs in the running group compared with the sedentary group (p < 0.05), and 16 miRNAs were significantly altered (p < 0.05) in the running group. Furthermore, functional annotation analysis of differentially expressed miRNA-predicted target genes showed that many of these target genes are involved in the PI3K-Akt pathway. Exercise-induced serum EDs increased Neuro2A cell viability and Akt phosphorylation. We also found that expression levels of neuronal maturation markers such as Microtubule-Associated Protein 2 (MAP2ab) and Neuronal nuclei (NeuN) were increased (p < 0.05, respectively), and that inhibition of the PI3K-Akt pathway by LY294002 pre-treatment ameliorated their expression in Neuro2A cells. Finally, the administration of exercise-induced EDs for 3 days increased the Histone 3 phosphorylation and β-III tubulin expression in Ink/Arf null neural stem cells and progenitors (NSPCs) under each proliferation and differentiation condition. These results suggest that exercise-induced circulating EDs may mediate neuronal maturation during exercise. Nature Publishing Group UK 2020-03-03 /pmc/articles/PMC7054262/ /pubmed/32127592 http://dx.doi.org/10.1038/s41598-020-60930-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Moon, Hyo Youl Yoon, Kyeong Jin Lee, Won Sang Cho, Hae-Sung Kim, Do-Yeon Kim, Ji-Seok Neural maturation enhanced by exercise-induced extracellular derivatives |
title | Neural maturation enhanced by exercise-induced extracellular derivatives |
title_full | Neural maturation enhanced by exercise-induced extracellular derivatives |
title_fullStr | Neural maturation enhanced by exercise-induced extracellular derivatives |
title_full_unstemmed | Neural maturation enhanced by exercise-induced extracellular derivatives |
title_short | Neural maturation enhanced by exercise-induced extracellular derivatives |
title_sort | neural maturation enhanced by exercise-induced extracellular derivatives |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054262/ https://www.ncbi.nlm.nih.gov/pubmed/32127592 http://dx.doi.org/10.1038/s41598-020-60930-6 |
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