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Studying signal compartmentation in adult cardiomyocytes

Multiple intra-cellular signalling pathways rely on calcium and 3′–5′ cyclic adenosine monophosphate (cAMP) to act as secondary messengers. This is especially true in cardiomyocytes which act as the force-producing units of the cardiac muscle and are required to react rapidly to environmental stimul...

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Detalles Bibliográficos
Autores principales: Judina, Aleksandra, Gorelik, Julia, Wright, Peter T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054744/
https://www.ncbi.nlm.nih.gov/pubmed/32104883
http://dx.doi.org/10.1042/BST20190247
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author Judina, Aleksandra
Gorelik, Julia
Wright, Peter T.
author_facet Judina, Aleksandra
Gorelik, Julia
Wright, Peter T.
author_sort Judina, Aleksandra
collection PubMed
description Multiple intra-cellular signalling pathways rely on calcium and 3′–5′ cyclic adenosine monophosphate (cAMP) to act as secondary messengers. This is especially true in cardiomyocytes which act as the force-producing units of the cardiac muscle and are required to react rapidly to environmental stimuli. The specificity of functional responses within cardiomyocytes and other cell types is produced by the organellar compartmentation of both calcium and cAMP. In this review, we assess the role of molecular localisation and relative contribution of active and passive processes in producing compartmentation. Active processes comprise the creation and destruction of signals, whereas passive processes comprise the release or sequestration of signals. Cardiomyocytes display a highly articulated membrane structure which displays significant cell-to-cell variability. Special attention is paid to the way in which cell membrane caveolae and the transverse-axial tubule system allow molecular localisation. We explore the effects of cell maturation, pathology and regional differences in the organisation of these processes. The subject of signal compartmentation has had a significant amount of attention within the cardiovascular field and has undergone a revolution over the past two decades. Advances in the area have been driven by molecular imaging using fluorescent dyes and genetically encoded constructs based upon fluorescent proteins. We also explore the use of scanning probe microscopy in the area. These techniques allow the analysis of molecular compartmentation within specific organellar compartments which gives researchers an entirely new perspective.
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spelling pubmed-70547442020-03-11 Studying signal compartmentation in adult cardiomyocytes Judina, Aleksandra Gorelik, Julia Wright, Peter T. Biochem Soc Trans Review Articles Multiple intra-cellular signalling pathways rely on calcium and 3′–5′ cyclic adenosine monophosphate (cAMP) to act as secondary messengers. This is especially true in cardiomyocytes which act as the force-producing units of the cardiac muscle and are required to react rapidly to environmental stimuli. The specificity of functional responses within cardiomyocytes and other cell types is produced by the organellar compartmentation of both calcium and cAMP. In this review, we assess the role of molecular localisation and relative contribution of active and passive processes in producing compartmentation. Active processes comprise the creation and destruction of signals, whereas passive processes comprise the release or sequestration of signals. Cardiomyocytes display a highly articulated membrane structure which displays significant cell-to-cell variability. Special attention is paid to the way in which cell membrane caveolae and the transverse-axial tubule system allow molecular localisation. We explore the effects of cell maturation, pathology and regional differences in the organisation of these processes. The subject of signal compartmentation has had a significant amount of attention within the cardiovascular field and has undergone a revolution over the past two decades. Advances in the area have been driven by molecular imaging using fluorescent dyes and genetically encoded constructs based upon fluorescent proteins. We also explore the use of scanning probe microscopy in the area. These techniques allow the analysis of molecular compartmentation within specific organellar compartments which gives researchers an entirely new perspective. Portland Press Ltd. 2020-02-28 2020-02-27 /pmc/articles/PMC7054744/ /pubmed/32104883 http://dx.doi.org/10.1042/BST20190247 Text en © 2020 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . Open access for this article was enabled by the participation of Imperial College London in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with JISC.
spellingShingle Review Articles
Judina, Aleksandra
Gorelik, Julia
Wright, Peter T.
Studying signal compartmentation in adult cardiomyocytes
title Studying signal compartmentation in adult cardiomyocytes
title_full Studying signal compartmentation in adult cardiomyocytes
title_fullStr Studying signal compartmentation in adult cardiomyocytes
title_full_unstemmed Studying signal compartmentation in adult cardiomyocytes
title_short Studying signal compartmentation in adult cardiomyocytes
title_sort studying signal compartmentation in adult cardiomyocytes
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054744/
https://www.ncbi.nlm.nih.gov/pubmed/32104883
http://dx.doi.org/10.1042/BST20190247
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