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MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

Nasopharyngeal carcinoma (NPC) causes severe oncogenic lesions in the nasopharynx. CD47, a transmembrane integrin-associated protein, plays a key role in the ability of tumor cells to escape phagocytosis, working as an immune checkpoint in the immune response. Besides this role, CD47 has been report...

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Autores principales: Zhao, Yunteng, Yu, Xiaoxiao, Tang, Haocheng, Han, Ri, Wang, Xianwen, Wang, Jianqi, Wang, Ke, Li, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054800/
https://www.ncbi.nlm.nih.gov/pubmed/32149101
http://dx.doi.org/10.1155/2020/3723781
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author Zhao, Yunteng
Yu, Xiaoxiao
Tang, Haocheng
Han, Ri
Wang, Xianwen
Wang, Jianqi
Wang, Ke
Li, Gang
author_facet Zhao, Yunteng
Yu, Xiaoxiao
Tang, Haocheng
Han, Ri
Wang, Xianwen
Wang, Jianqi
Wang, Ke
Li, Gang
author_sort Zhao, Yunteng
collection PubMed
description Nasopharyngeal carcinoma (NPC) causes severe oncogenic lesions in the nasopharynx. CD47, a transmembrane integrin-associated protein, plays a key role in the ability of tumor cells to escape phagocytosis, working as an immune checkpoint in the immune response. Besides this role, CD47 has been reported to regulate cell proliferation and migration. The present study addresses the relationship between CD47 and microRNA-200a and examines their regulatory mechanisms in NPC. Bioinformatics analyses and dual-luciferase reporter assays were used to confirm the putative relationship between miR-200a and CD47, and their interaction was further detected using western blotting and RT-PCR. Further, results showed that miR-200a affect NPC cell proliferation, migration, and invasion by regulating CD47. A cell phagocytosis assay showed that miR-200a and a CD47 monoclonal antibody increased the sensitivity of NPC cells to macrophage phagocytosis by inhibiting the functions of CD47. Additionally, miR-200a expression was suppressed and CD47 expression increased in both clinical NPC tissues and cell lines. Taken together, these results show the miR-200a/CD47 combination as a potential therapeutic for treatment of NPC.
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spelling pubmed-70548002020-03-08 MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47 Zhao, Yunteng Yu, Xiaoxiao Tang, Haocheng Han, Ri Wang, Xianwen Wang, Jianqi Wang, Ke Li, Gang Biomed Res Int Research Article Nasopharyngeal carcinoma (NPC) causes severe oncogenic lesions in the nasopharynx. CD47, a transmembrane integrin-associated protein, plays a key role in the ability of tumor cells to escape phagocytosis, working as an immune checkpoint in the immune response. Besides this role, CD47 has been reported to regulate cell proliferation and migration. The present study addresses the relationship between CD47 and microRNA-200a and examines their regulatory mechanisms in NPC. Bioinformatics analyses and dual-luciferase reporter assays were used to confirm the putative relationship between miR-200a and CD47, and their interaction was further detected using western blotting and RT-PCR. Further, results showed that miR-200a affect NPC cell proliferation, migration, and invasion by regulating CD47. A cell phagocytosis assay showed that miR-200a and a CD47 monoclonal antibody increased the sensitivity of NPC cells to macrophage phagocytosis by inhibiting the functions of CD47. Additionally, miR-200a expression was suppressed and CD47 expression increased in both clinical NPC tissues and cell lines. Taken together, these results show the miR-200a/CD47 combination as a potential therapeutic for treatment of NPC. Hindawi 2020-02-20 /pmc/articles/PMC7054800/ /pubmed/32149101 http://dx.doi.org/10.1155/2020/3723781 Text en Copyright © 2020 Yunteng Zhao et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Yunteng
Yu, Xiaoxiao
Tang, Haocheng
Han, Ri
Wang, Xianwen
Wang, Jianqi
Wang, Ke
Li, Gang
MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47
title MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47
title_full MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47
title_fullStr MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47
title_full_unstemmed MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47
title_short MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47
title_sort microrna-200a promotes phagocytosis of macrophages and suppresses cell proliferation, migration, and invasion in nasopharyngeal carcinoma by targeting cd47
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054800/
https://www.ncbi.nlm.nih.gov/pubmed/32149101
http://dx.doi.org/10.1155/2020/3723781
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