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PTH: Redefining Reference Ranges in a Healthy Population—The Role of Interfering Factors and the Type of Laboratory Assay

Introduction. Parathyroid hormone (PTH) is a linear peptide constituted by 84 amino acids and active in its 1–84 form, but a wide range of PTH forms produced by its post-transcriptional modifications are present in blood. Many assays with different specificities are commercially available. The aim o...

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Autores principales: Censi, Simona, Iacobone, Maurizio, Simmini, Stefano, Manso, Jacopo, Franceschet, Giulio, Plebani, Mario, Frigo, Anna Chiara, Zaninotto, Martina, Torresan, Francesca, De Silvestro, Giustina, Scaroni, Carla, Mian, Caterina, Camozzi, Valentina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054804/
https://www.ncbi.nlm.nih.gov/pubmed/32148482
http://dx.doi.org/10.1155/2020/1053719
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author Censi, Simona
Iacobone, Maurizio
Simmini, Stefano
Manso, Jacopo
Franceschet, Giulio
Plebani, Mario
Frigo, Anna Chiara
Zaninotto, Martina
Torresan, Francesca
De Silvestro, Giustina
Scaroni, Carla
Mian, Caterina
Camozzi, Valentina
author_facet Censi, Simona
Iacobone, Maurizio
Simmini, Stefano
Manso, Jacopo
Franceschet, Giulio
Plebani, Mario
Frigo, Anna Chiara
Zaninotto, Martina
Torresan, Francesca
De Silvestro, Giustina
Scaroni, Carla
Mian, Caterina
Camozzi, Valentina
author_sort Censi, Simona
collection PubMed
description Introduction. Parathyroid hormone (PTH) is a linear peptide constituted by 84 amino acids and active in its 1–84 form, but a wide range of PTH forms produced by its post-transcriptional modifications are present in blood. Many assays with different specificities are commercially available. The aim of our study was to compare a 2(nd) and 3(rd) generation in healthy population in order to better define the reference range in the healthy population residing in our region. Materials and Methods. 108 subjects (53 females and 55 males) referring to the transfusion donor were enrolled in the study centre in April 2016 and underwent PTH levels measurements with a 3(rd) generation kit (chemiluminescent immunoassay DiaSorin Liaison) and with a 2(nd) generation kit (immunoradiometric assay Total Intact PTH Assay (Coated Tube), Scantibodies). Also calcium, phosphate, creatinine, and 25OHD3 were measured. A questionnaire on lifestyle and dietary habits was obtained. RESULTS: The median PTH values obtained with the 2(nd) generation assay and the whole 3(rd) generation assay were 20.26 pg/ml and 23.11 pg/ml, respectively. Bland–Altman method showed substantial concordance between the two PTH assays, although with an overestimation of the 3(rd) generation method over the 2(nd) generation method. There was no correlation between 3(rd) generation PTH and 25OHD3 and creatinine. Calcium was negatively correlated with PTH only when measured with 3(rd) generation kit. CONCLUSIONS: On the basis of our data, obtained from healthy subjects, we can conclude that the reference range used by our laboratory was too narrow and was necessary to reestablish normal ranges according to our population. This is useful to avoid hyperparathyroidism misdiagnosis.
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spelling pubmed-70548042020-03-08 PTH: Redefining Reference Ranges in a Healthy Population—The Role of Interfering Factors and the Type of Laboratory Assay Censi, Simona Iacobone, Maurizio Simmini, Stefano Manso, Jacopo Franceschet, Giulio Plebani, Mario Frigo, Anna Chiara Zaninotto, Martina Torresan, Francesca De Silvestro, Giustina Scaroni, Carla Mian, Caterina Camozzi, Valentina Int J Endocrinol Research Article Introduction. Parathyroid hormone (PTH) is a linear peptide constituted by 84 amino acids and active in its 1–84 form, but a wide range of PTH forms produced by its post-transcriptional modifications are present in blood. Many assays with different specificities are commercially available. The aim of our study was to compare a 2(nd) and 3(rd) generation in healthy population in order to better define the reference range in the healthy population residing in our region. Materials and Methods. 108 subjects (53 females and 55 males) referring to the transfusion donor were enrolled in the study centre in April 2016 and underwent PTH levels measurements with a 3(rd) generation kit (chemiluminescent immunoassay DiaSorin Liaison) and with a 2(nd) generation kit (immunoradiometric assay Total Intact PTH Assay (Coated Tube), Scantibodies). Also calcium, phosphate, creatinine, and 25OHD3 were measured. A questionnaire on lifestyle and dietary habits was obtained. RESULTS: The median PTH values obtained with the 2(nd) generation assay and the whole 3(rd) generation assay were 20.26 pg/ml and 23.11 pg/ml, respectively. Bland–Altman method showed substantial concordance between the two PTH assays, although with an overestimation of the 3(rd) generation method over the 2(nd) generation method. There was no correlation between 3(rd) generation PTH and 25OHD3 and creatinine. Calcium was negatively correlated with PTH only when measured with 3(rd) generation kit. CONCLUSIONS: On the basis of our data, obtained from healthy subjects, we can conclude that the reference range used by our laboratory was too narrow and was necessary to reestablish normal ranges according to our population. This is useful to avoid hyperparathyroidism misdiagnosis. Hindawi 2020-02-21 /pmc/articles/PMC7054804/ /pubmed/32148482 http://dx.doi.org/10.1155/2020/1053719 Text en Copyright © 2020 Simona Censi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Censi, Simona
Iacobone, Maurizio
Simmini, Stefano
Manso, Jacopo
Franceschet, Giulio
Plebani, Mario
Frigo, Anna Chiara
Zaninotto, Martina
Torresan, Francesca
De Silvestro, Giustina
Scaroni, Carla
Mian, Caterina
Camozzi, Valentina
PTH: Redefining Reference Ranges in a Healthy Population—The Role of Interfering Factors and the Type of Laboratory Assay
title PTH: Redefining Reference Ranges in a Healthy Population—The Role of Interfering Factors and the Type of Laboratory Assay
title_full PTH: Redefining Reference Ranges in a Healthy Population—The Role of Interfering Factors and the Type of Laboratory Assay
title_fullStr PTH: Redefining Reference Ranges in a Healthy Population—The Role of Interfering Factors and the Type of Laboratory Assay
title_full_unstemmed PTH: Redefining Reference Ranges in a Healthy Population—The Role of Interfering Factors and the Type of Laboratory Assay
title_short PTH: Redefining Reference Ranges in a Healthy Population—The Role of Interfering Factors and the Type of Laboratory Assay
title_sort pth: redefining reference ranges in a healthy population—the role of interfering factors and the type of laboratory assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054804/
https://www.ncbi.nlm.nih.gov/pubmed/32148482
http://dx.doi.org/10.1155/2020/1053719
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