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Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes

Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. Th...

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Autores principales: Gil-Ramírez, Alicia, Rosmark, Oskar, Spégel, Peter, Swärd, Karl, Westergren-Thorsson, Gunilla, Larsson-Callerfelt, Anna-Karin, Rodríguez-Meizoso, Irene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055267/
https://www.ncbi.nlm.nih.gov/pubmed/32132596
http://dx.doi.org/10.1038/s41598-020-60827-4
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author Gil-Ramírez, Alicia
Rosmark, Oskar
Spégel, Peter
Swärd, Karl
Westergren-Thorsson, Gunilla
Larsson-Callerfelt, Anna-Karin
Rodríguez-Meizoso, Irene
author_facet Gil-Ramírez, Alicia
Rosmark, Oskar
Spégel, Peter
Swärd, Karl
Westergren-Thorsson, Gunilla
Larsson-Callerfelt, Anna-Karin
Rodríguez-Meizoso, Irene
author_sort Gil-Ramírez, Alicia
collection PubMed
description Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. The most commonly used methods for decellularization are based on chemicals and enzymes combinations, which often damage the ECM and cause cytotoxic effects in vivo. Mild methods involving pressurized CO(2)-ethanol (EtOH)-based fluids, in a supercritical or near supercritical state, have been studied for decellularization of cardiovascular tissue, but results are controversial. Moreover, data are lacking on the amount and type of lipids remaining in the tissue. Here we show that pressurized CO(2)-EtOH-H(2)O fluids (average molar composition, Χ(CO2) 0.91) yielded close to complete removal of lipids from porcine pulmonary arteries, including a notably decrease of pro-inflammatory fatty acids. Pressurized CO(2)-limonene fluids (Χ(CO2) 0.88) and neat supercritical CO(2) (scCO(2)) achieved the removal of 90% of triacylglycerides. Moreover, treatment of tissue with pressurized CO(2)-limonene followed by enzyme treatment, resulted in efficient DNA removal. The structure of elastic fibers was preserved after pressurized treatment, regardless solvent composition. In conclusion, pressurized CO(2)-ethanol fluids offer an efficient tool for delipidation in bio-scaffold production, while pressurized CO(2)-limonene fluids facilitate subsequent enzymatic removal of DNA.
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spelling pubmed-70552672020-03-12 Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes Gil-Ramírez, Alicia Rosmark, Oskar Spégel, Peter Swärd, Karl Westergren-Thorsson, Gunilla Larsson-Callerfelt, Anna-Karin Rodríguez-Meizoso, Irene Sci Rep Article Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. The most commonly used methods for decellularization are based on chemicals and enzymes combinations, which often damage the ECM and cause cytotoxic effects in vivo. Mild methods involving pressurized CO(2)-ethanol (EtOH)-based fluids, in a supercritical or near supercritical state, have been studied for decellularization of cardiovascular tissue, but results are controversial. Moreover, data are lacking on the amount and type of lipids remaining in the tissue. Here we show that pressurized CO(2)-EtOH-H(2)O fluids (average molar composition, Χ(CO2) 0.91) yielded close to complete removal of lipids from porcine pulmonary arteries, including a notably decrease of pro-inflammatory fatty acids. Pressurized CO(2)-limonene fluids (Χ(CO2) 0.88) and neat supercritical CO(2) (scCO(2)) achieved the removal of 90% of triacylglycerides. Moreover, treatment of tissue with pressurized CO(2)-limonene followed by enzyme treatment, resulted in efficient DNA removal. The structure of elastic fibers was preserved after pressurized treatment, regardless solvent composition. In conclusion, pressurized CO(2)-ethanol fluids offer an efficient tool for delipidation in bio-scaffold production, while pressurized CO(2)-limonene fluids facilitate subsequent enzymatic removal of DNA. Nature Publishing Group UK 2020-03-04 /pmc/articles/PMC7055267/ /pubmed/32132596 http://dx.doi.org/10.1038/s41598-020-60827-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gil-Ramírez, Alicia
Rosmark, Oskar
Spégel, Peter
Swärd, Karl
Westergren-Thorsson, Gunilla
Larsson-Callerfelt, Anna-Karin
Rodríguez-Meizoso, Irene
Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes
title Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes
title_full Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes
title_fullStr Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes
title_full_unstemmed Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes
title_short Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes
title_sort pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055267/
https://www.ncbi.nlm.nih.gov/pubmed/32132596
http://dx.doi.org/10.1038/s41598-020-60827-4
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