Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1

Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be use...

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Detalles Bibliográficos
Autores principales: Zivanov, Jasenko, Nakane, Takanori, Scheres, Sjors H. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055373/
https://www.ncbi.nlm.nih.gov/pubmed/32148853
http://dx.doi.org/10.1107/S2052252520000081
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author Zivanov, Jasenko
Nakane, Takanori
Scheres, Sjors H. W.
author_facet Zivanov, Jasenko
Nakane, Takanori
Scheres, Sjors H. W.
author_sort Zivanov, Jasenko
collection PubMed
description Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be used to correct for these aberrations for any given cryo-EM data set, a posteriori. Using five publicly available data sets, it is shown that considering these aberrations improves the resolution of the 3D reconstruction when these effects are present. The methods are implemented in version 3.1 of the open-source software package RELION.
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spelling pubmed-70553732020-03-06 Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1 Zivanov, Jasenko Nakane, Takanori Scheres, Sjors H. W. IUCrJ Research Papers Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be used to correct for these aberrations for any given cryo-EM data set, a posteriori. Using five publicly available data sets, it is shown that considering these aberrations improves the resolution of the 3D reconstruction when these effects are present. The methods are implemented in version 3.1 of the open-source software package RELION. International Union of Crystallography 2020-02-11 /pmc/articles/PMC7055373/ /pubmed/32148853 http://dx.doi.org/10.1107/S2052252520000081 Text en © Jasenko Zivanov et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Zivanov, Jasenko
Nakane, Takanori
Scheres, Sjors H. W.
Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1
title Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1
title_full Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1
title_fullStr Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1
title_full_unstemmed Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1
title_short Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION-3.1
title_sort estimation of high-order aberrations and anisotropic magnification from cryo-em data sets in relion-3.1
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055373/
https://www.ncbi.nlm.nih.gov/pubmed/32148853
http://dx.doi.org/10.1107/S2052252520000081
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