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The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase
Single-particle electron cryo-microscopy (cryoEM) has undergone a ‘resolution revolution’ that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expressio...
Autores principales: | Joppe, Mirko, D’Imprima, Edoardo, Salustros, Nina, Paithankar, Karthik S., Vonck, Janet, Grininger, Martin, Kühlbrandt, Werner |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055384/ https://www.ncbi.nlm.nih.gov/pubmed/32148850 http://dx.doi.org/10.1107/S2052252519017366 |
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