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Tubulin-binding cofactor E-like (TBCEL), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization

Individual sperm cells are resolved from a syncytium during late step of spermiogenesis known as individualization, which is accomplished by an Individualization Complex (IC) composed of 64 investment cones. mulet encodes Tubulin-binding cofactor E-like (TBCEL), suggesting a role for microtubule dyn...

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Autores principales: Fabrizio, James J., Rollins, Janet, Bazinet, Christopher W., Wegener, Stephanie, Koziy, Iryna, Daniel, Rachel, Lombardo, Vincent, Pryce, Dwaine, Bharrat, Kavita, Innabi, Elissa, Villanobos, Marielle, Mendoza, Gabriela, Ferrara, Elisa, Rodway, Stephanie, Vicioso, Matthew, Siracusa, Victoria, Dailey, Erin, Pronovost, Justin, Innabi, Simon, Patel, Vrutant, DeSouza, Nicole, Quaranto, Danielle, Niknejad, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055396/
https://www.ncbi.nlm.nih.gov/pubmed/32033965
http://dx.doi.org/10.1242/bio.049080
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author Fabrizio, James J.
Rollins, Janet
Bazinet, Christopher W.
Wegener, Stephanie
Koziy, Iryna
Daniel, Rachel
Lombardo, Vincent
Pryce, Dwaine
Bharrat, Kavita
Innabi, Elissa
Villanobos, Marielle
Mendoza, Gabriela
Ferrara, Elisa
Rodway, Stephanie
Vicioso, Matthew
Siracusa, Victoria
Dailey, Erin
Pronovost, Justin
Innabi, Simon
Patel, Vrutant
DeSouza, Nicole
Quaranto, Danielle
Niknejad, Amir
author_facet Fabrizio, James J.
Rollins, Janet
Bazinet, Christopher W.
Wegener, Stephanie
Koziy, Iryna
Daniel, Rachel
Lombardo, Vincent
Pryce, Dwaine
Bharrat, Kavita
Innabi, Elissa
Villanobos, Marielle
Mendoza, Gabriela
Ferrara, Elisa
Rodway, Stephanie
Vicioso, Matthew
Siracusa, Victoria
Dailey, Erin
Pronovost, Justin
Innabi, Simon
Patel, Vrutant
DeSouza, Nicole
Quaranto, Danielle
Niknejad, Amir
author_sort Fabrizio, James J.
collection PubMed
description Individual sperm cells are resolved from a syncytium during late step of spermiogenesis known as individualization, which is accomplished by an Individualization Complex (IC) composed of 64 investment cones. mulet encodes Tubulin-binding cofactor E-like (TBCEL), suggesting a role for microtubule dynamics in individualization. Indeed, a population of ∼100 cytoplasmic microtubules fails to disappear in mulet mutant testes during spermatogenesis. This persistence, detected using epi-fluorescence and electron microscopy, suggests that removal of these microtubules by TBCEL is a prerequisite for individualization. Immunofluorescence reveals TBCEL expression in elongated spermatid cysts. In addition, testes from mulet mutant males were rescued to wild type using tubulin-Gal4 to drive TBCEL expression, indicating that the mutant phenotype is caused by the lack of TBCEL. Finally, RNAi driven by bam-GAL4 successfully phenocopied mulet, confirming that mulet is required in the germline for individualization. We propose a model in which the cytoplasmic microtubules serve as alternate tracks for investment cones in mulet mutant testes. This article has an associated First Person interview with the first author of the paper.
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spelling pubmed-70553962020-03-04 Tubulin-binding cofactor E-like (TBCEL), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization Fabrizio, James J. Rollins, Janet Bazinet, Christopher W. Wegener, Stephanie Koziy, Iryna Daniel, Rachel Lombardo, Vincent Pryce, Dwaine Bharrat, Kavita Innabi, Elissa Villanobos, Marielle Mendoza, Gabriela Ferrara, Elisa Rodway, Stephanie Vicioso, Matthew Siracusa, Victoria Dailey, Erin Pronovost, Justin Innabi, Simon Patel, Vrutant DeSouza, Nicole Quaranto, Danielle Niknejad, Amir Biol Open Research Article Individual sperm cells are resolved from a syncytium during late step of spermiogenesis known as individualization, which is accomplished by an Individualization Complex (IC) composed of 64 investment cones. mulet encodes Tubulin-binding cofactor E-like (TBCEL), suggesting a role for microtubule dynamics in individualization. Indeed, a population of ∼100 cytoplasmic microtubules fails to disappear in mulet mutant testes during spermatogenesis. This persistence, detected using epi-fluorescence and electron microscopy, suggests that removal of these microtubules by TBCEL is a prerequisite for individualization. Immunofluorescence reveals TBCEL expression in elongated spermatid cysts. In addition, testes from mulet mutant males were rescued to wild type using tubulin-Gal4 to drive TBCEL expression, indicating that the mutant phenotype is caused by the lack of TBCEL. Finally, RNAi driven by bam-GAL4 successfully phenocopied mulet, confirming that mulet is required in the germline for individualization. We propose a model in which the cytoplasmic microtubules serve as alternate tracks for investment cones in mulet mutant testes. This article has an associated First Person interview with the first author of the paper. The Company of Biologists Ltd 2020-02-26 /pmc/articles/PMC7055396/ /pubmed/32033965 http://dx.doi.org/10.1242/bio.049080 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Fabrizio, James J.
Rollins, Janet
Bazinet, Christopher W.
Wegener, Stephanie
Koziy, Iryna
Daniel, Rachel
Lombardo, Vincent
Pryce, Dwaine
Bharrat, Kavita
Innabi, Elissa
Villanobos, Marielle
Mendoza, Gabriela
Ferrara, Elisa
Rodway, Stephanie
Vicioso, Matthew
Siracusa, Victoria
Dailey, Erin
Pronovost, Justin
Innabi, Simon
Patel, Vrutant
DeSouza, Nicole
Quaranto, Danielle
Niknejad, Amir
Tubulin-binding cofactor E-like (TBCEL), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization
title Tubulin-binding cofactor E-like (TBCEL), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization
title_full Tubulin-binding cofactor E-like (TBCEL), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization
title_fullStr Tubulin-binding cofactor E-like (TBCEL), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization
title_full_unstemmed Tubulin-binding cofactor E-like (TBCEL), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization
title_short Tubulin-binding cofactor E-like (TBCEL), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization
title_sort tubulin-binding cofactor e-like (tbcel), the protein product of the mulet gene, is required in the germline for the regulation of inter-flagellar microtubule dynamics during spermatid individualization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7055396/
https://www.ncbi.nlm.nih.gov/pubmed/32033965
http://dx.doi.org/10.1242/bio.049080
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