Growth Kinetics of Gold Nanoparticle Formation from Glycated Hemoglobin

[Image: see text] Gold nanostructures have always been a subject of interest to physicists, chemists, and material scientists. Despite the extensive research associated with gold nanoparticles, their actual formation mechanism is still debatable. The nanoscale rearrangements leading to the formation of gold nanostructures of definite size and shape are contradictory. The study presented in here details out a mechanism for gold nanoparticle formation in the presence of a biological template. The kinetics of gold nanostructure formation was studied using glycated hemoglobin as a biological template as well as the reducing agent. Particle formation was studied in a time- and temperature-dependent manner using different biophysical techniques. Here, we report for the first time spontaneous formation of gold nanoflowers which gradually dissociates to form smaller spherical particles. In addition, our experiments conclusively substantiate the existing postulations on gold nanoparticle formation from relatively larger precursor structures of gold and contradict with the popular nucleation growth mechanism.