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Methylation analysis of MIR200 family in Mexican patients with colorectal cancer
The present study aimed to analyze the methylation pattern of the MIR200 family in the colorectal tissues and peripheral blood of colorectal cancer (CRC) patients. Previous informed consent, 102 samples of colorectal tissues (tumor and adjacent normal tissues) and 40 peripheral blood samples were co...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057793/ https://www.ncbi.nlm.nih.gov/pubmed/31874934 http://dx.doi.org/10.1136/jim-2019-001184 |
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author | Alvizo-Rodriguez, Carlos Rogelio Ayala-Madrigal, Maria de la Luz Hernandez-Sandoval, Jesus Arturo Ramirez-Plascencia, Helen Haydee Fernanda Gonzalez-Villaseñor, Christian Octavio Macias-Gomez, Nelly Margarita Peregrina-Sandoval, Jorge Moreno-Ortiz, Jose Miguel Valenzuela-Perez, Jesus A Cruz-Ramos, Jose Alfonso Gutierrez-Angulo, Melva |
author_facet | Alvizo-Rodriguez, Carlos Rogelio Ayala-Madrigal, Maria de la Luz Hernandez-Sandoval, Jesus Arturo Ramirez-Plascencia, Helen Haydee Fernanda Gonzalez-Villaseñor, Christian Octavio Macias-Gomez, Nelly Margarita Peregrina-Sandoval, Jorge Moreno-Ortiz, Jose Miguel Valenzuela-Perez, Jesus A Cruz-Ramos, Jose Alfonso Gutierrez-Angulo, Melva |
author_sort | Alvizo-Rodriguez, Carlos Rogelio |
collection | PubMed |
description | The present study aimed to analyze the methylation pattern of the MIR200 family in the colorectal tissues and peripheral blood of colorectal cancer (CRC) patients. Previous informed consent, 102 samples of colorectal tissues (tumor and adjacent normal tissues) and 40 peripheral blood samples were collected from CRC patients. Additionally, we included a reference group of 40 blood samples. DNA extraction was done for colorectal tissues and peripheral blood. For methylation-specific PCR, we used bisulfite-treated DNA and controls for methylated and unmethylated DNA were included to each assay. PCR fragments were separated by 6% polyacrylamide gel electrophoresis. Methylation-positive and methylation-negative results were confirmed by bisulfite genomic sequencing technique. We analyzed 102 colorectal tissues and 40 blood samples from 51 CRC patients. MIR200B/MIR200A/MIR429 methylation analysis discloses no differences among tissues (p>0.05). However, MIR200C/MIR141 methylation showed differences between colorectal tissues and peripheral blood of CRC patients (p<0.0001) and mainly methylated alleles were observed in peripheral blood. These findings suggest a tissue-specific methylation pattern for the MIR200C/MIR141 promoter. |
format | Online Article Text |
id | pubmed-7057793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-70577932020-03-17 Methylation analysis of MIR200 family in Mexican patients with colorectal cancer Alvizo-Rodriguez, Carlos Rogelio Ayala-Madrigal, Maria de la Luz Hernandez-Sandoval, Jesus Arturo Ramirez-Plascencia, Helen Haydee Fernanda Gonzalez-Villaseñor, Christian Octavio Macias-Gomez, Nelly Margarita Peregrina-Sandoval, Jorge Moreno-Ortiz, Jose Miguel Valenzuela-Perez, Jesus A Cruz-Ramos, Jose Alfonso Gutierrez-Angulo, Melva J Investig Med Original Research The present study aimed to analyze the methylation pattern of the MIR200 family in the colorectal tissues and peripheral blood of colorectal cancer (CRC) patients. Previous informed consent, 102 samples of colorectal tissues (tumor and adjacent normal tissues) and 40 peripheral blood samples were collected from CRC patients. Additionally, we included a reference group of 40 blood samples. DNA extraction was done for colorectal tissues and peripheral blood. For methylation-specific PCR, we used bisulfite-treated DNA and controls for methylated and unmethylated DNA were included to each assay. PCR fragments were separated by 6% polyacrylamide gel electrophoresis. Methylation-positive and methylation-negative results were confirmed by bisulfite genomic sequencing technique. We analyzed 102 colorectal tissues and 40 blood samples from 51 CRC patients. MIR200B/MIR200A/MIR429 methylation analysis discloses no differences among tissues (p>0.05). However, MIR200C/MIR141 methylation showed differences between colorectal tissues and peripheral blood of CRC patients (p<0.0001) and mainly methylated alleles were observed in peripheral blood. These findings suggest a tissue-specific methylation pattern for the MIR200C/MIR141 promoter. BMJ Publishing Group 2020-03 2019-12-23 /pmc/articles/PMC7057793/ /pubmed/31874934 http://dx.doi.org/10.1136/jim-2019-001184 Text en © American Federation for Medical Research 2020. Re-use permitted under CC BY-NC. No commercial re-use. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, an indication of whether changes were made, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Original Research Alvizo-Rodriguez, Carlos Rogelio Ayala-Madrigal, Maria de la Luz Hernandez-Sandoval, Jesus Arturo Ramirez-Plascencia, Helen Haydee Fernanda Gonzalez-Villaseñor, Christian Octavio Macias-Gomez, Nelly Margarita Peregrina-Sandoval, Jorge Moreno-Ortiz, Jose Miguel Valenzuela-Perez, Jesus A Cruz-Ramos, Jose Alfonso Gutierrez-Angulo, Melva Methylation analysis of MIR200 family in Mexican patients with colorectal cancer |
title | Methylation analysis of MIR200 family in Mexican patients with colorectal cancer |
title_full | Methylation analysis of MIR200 family in Mexican patients with colorectal cancer |
title_fullStr | Methylation analysis of MIR200 family in Mexican patients with colorectal cancer |
title_full_unstemmed | Methylation analysis of MIR200 family in Mexican patients with colorectal cancer |
title_short | Methylation analysis of MIR200 family in Mexican patients with colorectal cancer |
title_sort | methylation analysis of mir200 family in mexican patients with colorectal cancer |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057793/ https://www.ncbi.nlm.nih.gov/pubmed/31874934 http://dx.doi.org/10.1136/jim-2019-001184 |
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