MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3

BACKGROUND: In this study, we aimed to explore the potential involvement of miR-3150b in hepatocellular carcinoma (HCC) carcinogenesis. METHODS: The expression of miR-3150b and Golgi phosphoprotein 3 (GOLPH3) was determined in HCC cell lines. Cell proliferation, migration and invasion were estimated...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Yi, Wang, Jianjun, Su, Hongling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2020
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057801/
https://www.ncbi.nlm.nih.gov/pubmed/31806673
http://dx.doi.org/10.1136/jim-2019-001181
_version_ 1783503737342394368
author Zhang, Yi
Wang, Jianjun
Su, Hongling
author_facet Zhang, Yi
Wang, Jianjun
Su, Hongling
author_sort Zhang, Yi
collection PubMed
description BACKGROUND: In this study, we aimed to explore the potential involvement of miR-3150b in hepatocellular carcinoma (HCC) carcinogenesis. METHODS: The expression of miR-3150b and Golgi phosphoprotein 3 (GOLPH3) was determined in HCC cell lines. Cell proliferation, migration and invasion were estimated by Cell Counting Kit-8, wound healing and Transwell assays. The association between miR-3150b and GOLPH3 was verified by luciferase assay. RESULTS: MiR-3150b was downregulated, while GOLPH3 was remarkably upregulated in HCC cells. Furthermore, miR-3150b inhibited HCC cell proliferation, migration and invasion. MiR-3150b directly targeted and negatively regulated GOLPH3. CONCLUSION: MiR-3150b suppressed HCC cell proliferation, invasion and migration by targeting GOLPH3.
format Online
Article
Text
id pubmed-7057801
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BMJ Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-70578012020-03-17 MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3 Zhang, Yi Wang, Jianjun Su, Hongling J Investig Med Original Research BACKGROUND: In this study, we aimed to explore the potential involvement of miR-3150b in hepatocellular carcinoma (HCC) carcinogenesis. METHODS: The expression of miR-3150b and Golgi phosphoprotein 3 (GOLPH3) was determined in HCC cell lines. Cell proliferation, migration and invasion were estimated by Cell Counting Kit-8, wound healing and Transwell assays. The association between miR-3150b and GOLPH3 was verified by luciferase assay. RESULTS: MiR-3150b was downregulated, while GOLPH3 was remarkably upregulated in HCC cells. Furthermore, miR-3150b inhibited HCC cell proliferation, migration and invasion. MiR-3150b directly targeted and negatively regulated GOLPH3. CONCLUSION: MiR-3150b suppressed HCC cell proliferation, invasion and migration by targeting GOLPH3. BMJ Publishing Group 2020-03 2019-12-05 /pmc/articles/PMC7057801/ /pubmed/31806673 http://dx.doi.org/10.1136/jim-2019-001181 Text en © American Federation for Medical Research 2020. Re-use permitted under CC BY. Published by BMJ. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Research
Zhang, Yi
Wang, Jianjun
Su, Hongling
MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3
title MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3
title_full MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3
title_fullStr MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3
title_full_unstemmed MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3
title_short MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3
title_sort mir-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting golph3
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057801/
https://www.ncbi.nlm.nih.gov/pubmed/31806673
http://dx.doi.org/10.1136/jim-2019-001181
work_keys_str_mv AT zhangyi mir3150binhibitshepatocellularcarcinomacellproliferationmigrationandinvasionbytargetinggolph3
AT wangjianjun mir3150binhibitshepatocellularcarcinomacellproliferationmigrationandinvasionbytargetinggolph3
AT suhongling mir3150binhibitshepatocellularcarcinomacellproliferationmigrationandinvasionbytargetinggolph3

Ejemplares similares