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Reduced expression of exosomal miR-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement

The peritoneal surface is the most frequent site of metastasis disease in patients with gastric cancer. Even after curative surgery and adjuvant chemotherapy, peritoneal recurrences often develop. Exosomes play pivotal roles in tumor metastasis via the transfer of microRNAs (miRNAs). In the present...

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Autores principales: Ohzawa, Hideyuki, Saito, Akira, Kumagai, Yuko, Kimura, Yuki, Yamaguchi, Hironori, Hosoya, Yoshinori, Lefor, Alan Kawarai, Sata, Naohiro, Kitayama, Joji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057811/
https://www.ncbi.nlm.nih.gov/pubmed/32323853
http://dx.doi.org/10.3892/or.2020.7505
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author Ohzawa, Hideyuki
Saito, Akira
Kumagai, Yuko
Kimura, Yuki
Yamaguchi, Hironori
Hosoya, Yoshinori
Lefor, Alan Kawarai
Sata, Naohiro
Kitayama, Joji
author_facet Ohzawa, Hideyuki
Saito, Akira
Kumagai, Yuko
Kimura, Yuki
Yamaguchi, Hironori
Hosoya, Yoshinori
Lefor, Alan Kawarai
Sata, Naohiro
Kitayama, Joji
author_sort Ohzawa, Hideyuki
collection PubMed
description The peritoneal surface is the most frequent site of metastasis disease in patients with gastric cancer. Even after curative surgery and adjuvant chemotherapy, peritoneal recurrences often develop. Exosomes play pivotal roles in tumor metastasis via the transfer of microRNAs (miRNAs). In the present study, exosomes were isolated from peritoneal lavage fluid or ascites in 85 patients with gastric cancer and the relative expression levels of miR-29s were examined. The expression of miR-29a-3p, miR-29b-3p and miR-29c-3p in peritoneal exosomes were all downregulated in patients with peritoneal metastases (PM) compared to those without PM. In 30 patients who underwent curative gastrectomy with serosa-involved (T4) gastric cancer, 6 patients exhibited recurrence in the peritoneum within 12 months. The expression levels of miR-29s at gastrectomy tended to be lower in these 6 patients than in the other 24 patients with significant differences in miR-29b-3p (P=0.003). When the patients were divided into two groups based on median levels of miR-29s, peritoneal recurrence developed more frequently in patients with low expression of miR-29b-3p, and lower expression of miR-29s were related with worse overall survival. miR-29s are thought to play a suppressive role in the growth of disseminated peritoneal tumor cells. Reduced expression of miR-29b in peritoneal exosomes is a strong risk factor of developing postoperative peritoneal recurrence.
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spelling pubmed-70578112020-03-18 Reduced expression of exosomal miR-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement Ohzawa, Hideyuki Saito, Akira Kumagai, Yuko Kimura, Yuki Yamaguchi, Hironori Hosoya, Yoshinori Lefor, Alan Kawarai Sata, Naohiro Kitayama, Joji Oncol Rep Articles The peritoneal surface is the most frequent site of metastasis disease in patients with gastric cancer. Even after curative surgery and adjuvant chemotherapy, peritoneal recurrences often develop. Exosomes play pivotal roles in tumor metastasis via the transfer of microRNAs (miRNAs). In the present study, exosomes were isolated from peritoneal lavage fluid or ascites in 85 patients with gastric cancer and the relative expression levels of miR-29s were examined. The expression of miR-29a-3p, miR-29b-3p and miR-29c-3p in peritoneal exosomes were all downregulated in patients with peritoneal metastases (PM) compared to those without PM. In 30 patients who underwent curative gastrectomy with serosa-involved (T4) gastric cancer, 6 patients exhibited recurrence in the peritoneum within 12 months. The expression levels of miR-29s at gastrectomy tended to be lower in these 6 patients than in the other 24 patients with significant differences in miR-29b-3p (P=0.003). When the patients were divided into two groups based on median levels of miR-29s, peritoneal recurrence developed more frequently in patients with low expression of miR-29b-3p, and lower expression of miR-29s were related with worse overall survival. miR-29s are thought to play a suppressive role in the growth of disseminated peritoneal tumor cells. Reduced expression of miR-29b in peritoneal exosomes is a strong risk factor of developing postoperative peritoneal recurrence. D.A. Spandidos 2020-04 2020-02-17 /pmc/articles/PMC7057811/ /pubmed/32323853 http://dx.doi.org/10.3892/or.2020.7505 Text en Copyright: © Ohzawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ohzawa, Hideyuki
Saito, Akira
Kumagai, Yuko
Kimura, Yuki
Yamaguchi, Hironori
Hosoya, Yoshinori
Lefor, Alan Kawarai
Sata, Naohiro
Kitayama, Joji
Reduced expression of exosomal miR-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement
title Reduced expression of exosomal miR-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement
title_full Reduced expression of exosomal miR-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement
title_fullStr Reduced expression of exosomal miR-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement
title_full_unstemmed Reduced expression of exosomal miR-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement
title_short Reduced expression of exosomal miR-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement
title_sort reduced expression of exosomal mir-29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057811/
https://www.ncbi.nlm.nih.gov/pubmed/32323853
http://dx.doi.org/10.3892/or.2020.7505
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