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Phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets

Two experiments (Exp.) with ileally cannulated growing barrows were conducted. The concentrations of positional inositol phosphate (InsP) isomers in ileal digesta and feces were determined, as well as the prececal and total tract phytate (InsP(6)) hydrolysis, and digestibility of dry matter, P, Ca,...

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Autores principales: Rosenfelder-Kuon, Pia, Klein, Nicolas, Zegowitz, Benedikt, Schollenberger, Margit, Kühn, Imke, Thuringer, Lucia, Seifert, Jana, Rodehutscord, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057925/
https://www.ncbi.nlm.nih.gov/pubmed/32060531
http://dx.doi.org/10.1093/jas/skaa053
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author Rosenfelder-Kuon, Pia
Klein, Nicolas
Zegowitz, Benedikt
Schollenberger, Margit
Kühn, Imke
Thuringer, Lucia
Seifert, Jana
Rodehutscord, Markus
author_facet Rosenfelder-Kuon, Pia
Klein, Nicolas
Zegowitz, Benedikt
Schollenberger, Margit
Kühn, Imke
Thuringer, Lucia
Seifert, Jana
Rodehutscord, Markus
author_sort Rosenfelder-Kuon, Pia
collection PubMed
description Two experiments (Exp.) with ileally cannulated growing barrows were conducted. The concentrations of positional inositol phosphate (InsP) isomers in ileal digesta and feces were determined, as well as the prececal and total tract phytate (InsP(6)) hydrolysis, and digestibility of dry matter, P, Ca, nitrogen, and gross energy. Prececal amino acid (AA) digestibility and digestive enzyme activities in ileal digesta were also studied. In both Exp., pigs had an initial body weight (BW) of 28 kg and were completely randomized to a Double Latin Square Design with eight pigs, four diets, and three periods of 12 d each. Feces and ileal digesta were collected for 5 d and 2 d, respectively. Pigs were housed individually in stainless steel metabolic units. Water was available ad libitum and feed was provided two times daily at an amount of 4% of mean BW. In Exp. 1, pigs received a corn–soybean meal (SBM)-based diet that was supplemented with 0, 750, 1,500, or 3,000 FTU of a microbial phytase/kg diet. In Exp. 2, pigs were allotted to a 2 × 2 arrangement of diets based on corn and SBM or an SBM-rapeseed cake (RSC) mix and phytase supplementation at 0 or 1,500 FTU/kg of diet. In ileal digesta of pigs fed without the phytase supplement, the dominating InsP isomers beside InsP(6) were InsP(5) isomers. The InsP pattern in ileal digesta changed with the inclusion of microbial phytase in both Exp., as there was a remarkable increase in Ins(1,2,5,6)P(4) concentration (P < 0.001). In both Exp., the myo-inositol concentration in ileal digesta was greater upon phytase addition (P < 0.001). Without phytase supplementation, prececal and total tract P digestibility were low, whereas hardly any InsP(6) was excreted in feces. There was no difference between prececal and total tract P digestibility values. For most AA studied in Exp. 2, prececal digestibility was lower (P < 0.01) when the diet contained RSC. However, phytase supplementation did not significantly affect prececal AA digestibility in both Exp. The present study showed that InsP(6) disappearance by the end of the ileum can be increased up to around 90% in SBM- and SBM–RSC-based diets when microbial phytase is supplemented, but prececal P digestibility hardly exceeded 60%. The study confirms that pigs cannot benefit from a remarkable InsP(6) degradation in the hindgut.
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spelling pubmed-70579252020-03-10 Phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets Rosenfelder-Kuon, Pia Klein, Nicolas Zegowitz, Benedikt Schollenberger, Margit Kühn, Imke Thuringer, Lucia Seifert, Jana Rodehutscord, Markus J Anim Sci Non Ruminant Nutrition Two experiments (Exp.) with ileally cannulated growing barrows were conducted. The concentrations of positional inositol phosphate (InsP) isomers in ileal digesta and feces were determined, as well as the prececal and total tract phytate (InsP(6)) hydrolysis, and digestibility of dry matter, P, Ca, nitrogen, and gross energy. Prececal amino acid (AA) digestibility and digestive enzyme activities in ileal digesta were also studied. In both Exp., pigs had an initial body weight (BW) of 28 kg and were completely randomized to a Double Latin Square Design with eight pigs, four diets, and three periods of 12 d each. Feces and ileal digesta were collected for 5 d and 2 d, respectively. Pigs were housed individually in stainless steel metabolic units. Water was available ad libitum and feed was provided two times daily at an amount of 4% of mean BW. In Exp. 1, pigs received a corn–soybean meal (SBM)-based diet that was supplemented with 0, 750, 1,500, or 3,000 FTU of a microbial phytase/kg diet. In Exp. 2, pigs were allotted to a 2 × 2 arrangement of diets based on corn and SBM or an SBM-rapeseed cake (RSC) mix and phytase supplementation at 0 or 1,500 FTU/kg of diet. In ileal digesta of pigs fed without the phytase supplement, the dominating InsP isomers beside InsP(6) were InsP(5) isomers. The InsP pattern in ileal digesta changed with the inclusion of microbial phytase in both Exp., as there was a remarkable increase in Ins(1,2,5,6)P(4) concentration (P < 0.001). In both Exp., the myo-inositol concentration in ileal digesta was greater upon phytase addition (P < 0.001). Without phytase supplementation, prececal and total tract P digestibility were low, whereas hardly any InsP(6) was excreted in feces. There was no difference between prececal and total tract P digestibility values. For most AA studied in Exp. 2, prececal digestibility was lower (P < 0.01) when the diet contained RSC. However, phytase supplementation did not significantly affect prececal AA digestibility in both Exp. The present study showed that InsP(6) disappearance by the end of the ileum can be increased up to around 90% in SBM- and SBM–RSC-based diets when microbial phytase is supplemented, but prececal P digestibility hardly exceeded 60%. The study confirms that pigs cannot benefit from a remarkable InsP(6) degradation in the hindgut. Oxford University Press 2020-02-15 /pmc/articles/PMC7057925/ /pubmed/32060531 http://dx.doi.org/10.1093/jas/skaa053 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the American Society of Animal Science. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Non Ruminant Nutrition
Rosenfelder-Kuon, Pia
Klein, Nicolas
Zegowitz, Benedikt
Schollenberger, Margit
Kühn, Imke
Thuringer, Lucia
Seifert, Jana
Rodehutscord, Markus
Phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets
title Phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets
title_full Phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets
title_fullStr Phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets
title_full_unstemmed Phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets
title_short Phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets
title_sort phytate degradation cascade in pigs as affected by phytase supplementation and rapeseed cake inclusion in corn–soybean meal-based diets
topic Non Ruminant Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057925/
https://www.ncbi.nlm.nih.gov/pubmed/32060531
http://dx.doi.org/10.1093/jas/skaa053
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