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Identification of region-specific astrocyte subtypes at single cell resolution
Astrocytes, a major cell type found throughout the central nervous system, have general roles in the modulation of synapse formation and synaptic transmission, blood–brain barrier formation, and regulation of blood flow, as well as metabolic support of other brain resident cells. Crucially, emerging...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058027/ https://www.ncbi.nlm.nih.gov/pubmed/32139688 http://dx.doi.org/10.1038/s41467-019-14198-8 |
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author | Batiuk, Mykhailo Y. Martirosyan, Araks Wahis, Jérôme de Vin, Filip Marneffe, Catherine Kusserow, Carola Koeppen, Jordan Viana, João Filipe Oliveira, João Filipe Voet, Thierry Ponting, Chris P. Belgard, T. Grant Holt, Matthew G. |
author_facet | Batiuk, Mykhailo Y. Martirosyan, Araks Wahis, Jérôme de Vin, Filip Marneffe, Catherine Kusserow, Carola Koeppen, Jordan Viana, João Filipe Oliveira, João Filipe Voet, Thierry Ponting, Chris P. Belgard, T. Grant Holt, Matthew G. |
author_sort | Batiuk, Mykhailo Y. |
collection | PubMed |
description | Astrocytes, a major cell type found throughout the central nervous system, have general roles in the modulation of synapse formation and synaptic transmission, blood–brain barrier formation, and regulation of blood flow, as well as metabolic support of other brain resident cells. Crucially, emerging evidence shows specific adaptations and astrocyte-encoded functions in regions, such as the spinal cord and cerebellum. To investigate the true extent of astrocyte molecular diversity across forebrain regions, we used single-cell RNA sequencing. Our analysis identifies five transcriptomically distinct astrocyte subtypes in adult mouse cortex and hippocampus. Validation of our data in situ reveals distinct spatial positioning of defined subtypes, reflecting the distribution of morphologically and physiologically distinct astrocyte populations. Our findings are evidence for specialized astrocyte subtypes between and within brain regions. The data are available through an online database (https://holt-sc.glialab.org/), providing a resource on which to base explorations of local astrocyte diversity and function in the brain. |
format | Online Article Text |
id | pubmed-7058027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70580272020-03-06 Identification of region-specific astrocyte subtypes at single cell resolution Batiuk, Mykhailo Y. Martirosyan, Araks Wahis, Jérôme de Vin, Filip Marneffe, Catherine Kusserow, Carola Koeppen, Jordan Viana, João Filipe Oliveira, João Filipe Voet, Thierry Ponting, Chris P. Belgard, T. Grant Holt, Matthew G. Nat Commun Article Astrocytes, a major cell type found throughout the central nervous system, have general roles in the modulation of synapse formation and synaptic transmission, blood–brain barrier formation, and regulation of blood flow, as well as metabolic support of other brain resident cells. Crucially, emerging evidence shows specific adaptations and astrocyte-encoded functions in regions, such as the spinal cord and cerebellum. To investigate the true extent of astrocyte molecular diversity across forebrain regions, we used single-cell RNA sequencing. Our analysis identifies five transcriptomically distinct astrocyte subtypes in adult mouse cortex and hippocampus. Validation of our data in situ reveals distinct spatial positioning of defined subtypes, reflecting the distribution of morphologically and physiologically distinct astrocyte populations. Our findings are evidence for specialized astrocyte subtypes between and within brain regions. The data are available through an online database (https://holt-sc.glialab.org/), providing a resource on which to base explorations of local astrocyte diversity and function in the brain. Nature Publishing Group UK 2020-03-05 /pmc/articles/PMC7058027/ /pubmed/32139688 http://dx.doi.org/10.1038/s41467-019-14198-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Batiuk, Mykhailo Y. Martirosyan, Araks Wahis, Jérôme de Vin, Filip Marneffe, Catherine Kusserow, Carola Koeppen, Jordan Viana, João Filipe Oliveira, João Filipe Voet, Thierry Ponting, Chris P. Belgard, T. Grant Holt, Matthew G. Identification of region-specific astrocyte subtypes at single cell resolution |
title | Identification of region-specific astrocyte subtypes at single cell resolution |
title_full | Identification of region-specific astrocyte subtypes at single cell resolution |
title_fullStr | Identification of region-specific astrocyte subtypes at single cell resolution |
title_full_unstemmed | Identification of region-specific astrocyte subtypes at single cell resolution |
title_short | Identification of region-specific astrocyte subtypes at single cell resolution |
title_sort | identification of region-specific astrocyte subtypes at single cell resolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058027/ https://www.ncbi.nlm.nih.gov/pubmed/32139688 http://dx.doi.org/10.1038/s41467-019-14198-8 |
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