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A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage

Bacterial type III secretion systems (T3SSs) play an important role in pathogenesis of Gram-negative infections. Enteropathogenic and enterohemorrhagic Escherichia coli contain a well-defined T3SS but in addition a second T3SS termed E. coli T3SS 2 (ETT2) has been described in a number of strains of...

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Autores principales: Fox, Stephen, Goswami, Cosmika, Holden, Matthew, Connolly, James P. R., Mordue, James, O’Boyle, Nicky, Roe, Andrew, Connor, Martin, Leanord, Alistair, Evans, Tom J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058095/
https://www.ncbi.nlm.nih.gov/pubmed/32139768
http://dx.doi.org/10.1038/s41598-020-61026-x
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author Fox, Stephen
Goswami, Cosmika
Holden, Matthew
Connolly, James P. R.
Mordue, James
O’Boyle, Nicky
Roe, Andrew
Connor, Martin
Leanord, Alistair
Evans, Tom J.
author_facet Fox, Stephen
Goswami, Cosmika
Holden, Matthew
Connolly, James P. R.
Mordue, James
O’Boyle, Nicky
Roe, Andrew
Connor, Martin
Leanord, Alistair
Evans, Tom J.
author_sort Fox, Stephen
collection PubMed
description Bacterial type III secretion systems (T3SSs) play an important role in pathogenesis of Gram-negative infections. Enteropathogenic and enterohemorrhagic Escherichia coli contain a well-defined T3SS but in addition a second T3SS termed E. coli T3SS 2 (ETT2) has been described in a number of strains of E. coli. The majority of pathogenic E. coli contain elements of a genetic locus encoding ETT2, but which has undergone significant mutational attrition rendering it without predicted function. Only a very few strains have been reported to contain an intact ETT2 locus. To investigate the occurrence of the ETT2 locus in strains of human pathogenic E. coli, we carried out genomic sequencing of 162 isolates obtained from patient blood cultures in Scotland. We found that 22 of 26 sequence type (ST) 69 isolates from this collection contained an intact ETT2 together with an associated eip locus which encodes putative secreted ETT2 effectors as well as eilA, a gene encoding a putative transcriptional regulator of ETT2 associated genes. Using a reporter gene for eilA activation, we defined conditions under which this gene was differentially activated. Analysis of published E. coli genomes with worldwide representation showed that ST69 contained an intact ETT2 in these strains as well. The conservation of the genes encoding ETT2 in human pathogenic ST69 strains strongly suggests it has importance in infection, although its exact functional role remains obscure.
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spelling pubmed-70580952020-03-12 A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage Fox, Stephen Goswami, Cosmika Holden, Matthew Connolly, James P. R. Mordue, James O’Boyle, Nicky Roe, Andrew Connor, Martin Leanord, Alistair Evans, Tom J. Sci Rep Article Bacterial type III secretion systems (T3SSs) play an important role in pathogenesis of Gram-negative infections. Enteropathogenic and enterohemorrhagic Escherichia coli contain a well-defined T3SS but in addition a second T3SS termed E. coli T3SS 2 (ETT2) has been described in a number of strains of E. coli. The majority of pathogenic E. coli contain elements of a genetic locus encoding ETT2, but which has undergone significant mutational attrition rendering it without predicted function. Only a very few strains have been reported to contain an intact ETT2 locus. To investigate the occurrence of the ETT2 locus in strains of human pathogenic E. coli, we carried out genomic sequencing of 162 isolates obtained from patient blood cultures in Scotland. We found that 22 of 26 sequence type (ST) 69 isolates from this collection contained an intact ETT2 together with an associated eip locus which encodes putative secreted ETT2 effectors as well as eilA, a gene encoding a putative transcriptional regulator of ETT2 associated genes. Using a reporter gene for eilA activation, we defined conditions under which this gene was differentially activated. Analysis of published E. coli genomes with worldwide representation showed that ST69 contained an intact ETT2 in these strains as well. The conservation of the genes encoding ETT2 in human pathogenic ST69 strains strongly suggests it has importance in infection, although its exact functional role remains obscure. Nature Publishing Group UK 2020-03-05 /pmc/articles/PMC7058095/ /pubmed/32139768 http://dx.doi.org/10.1038/s41598-020-61026-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fox, Stephen
Goswami, Cosmika
Holden, Matthew
Connolly, James P. R.
Mordue, James
O’Boyle, Nicky
Roe, Andrew
Connor, Martin
Leanord, Alistair
Evans, Tom J.
A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage
title A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage
title_full A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage
title_fullStr A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage
title_full_unstemmed A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage
title_short A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage
title_sort highly conserved complete accessory escherichia coli type iii secretion system 2 is widespread in bloodstream isolates of the st69 lineage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058095/
https://www.ncbi.nlm.nih.gov/pubmed/32139768
http://dx.doi.org/10.1038/s41598-020-61026-x
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