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A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system
INTRODUCTION: The objective of this study is to design a cancer invasion model where the cancer invasion rate can be regulated in vitro. METHODS: Cancer-associated fibroblasts (CAF) aggregates incorporating gelatin hydrogel microspheres (GM) containing various concentrations of transforming growth f...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society for Regenerative Medicine
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058408/ https://www.ncbi.nlm.nih.gov/pubmed/32154334 http://dx.doi.org/10.1016/j.reth.2020.02.003 |
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author | Nii, Teruki Makino, Kimiko Tabata, Yasuhiko |
author_facet | Nii, Teruki Makino, Kimiko Tabata, Yasuhiko |
author_sort | Nii, Teruki |
collection | PubMed |
description | INTRODUCTION: The objective of this study is to design a cancer invasion model where the cancer invasion rate can be regulated in vitro. METHODS: Cancer-associated fibroblasts (CAF) aggregates incorporating gelatin hydrogel microspheres (GM) containing various concentrations of transforming growth factor-β1 (TGF-β1) (CAF-GM-TGF-β1) were prepared. Alpha-smooth muscle actin (α-SMA) for the CAF aggregates was measured to investigate the CAF activation level by changing the concentration of TGF-β1. An invasion assay was performed to evaluate the cancer invasion rate by co-cultured of cancer cells with various CAF-GM-TGF-β1. RESULTS: The expression level of α-SMA for CAF increased with an increased in the TGF-β1 concentration. When co-cultured with various types of CAF-GM-TGF-β1, the cancer invasion rate was well correlated with the α-SMA level. It is conceivable that the TGF-β1 concentration could modify the level of CAF activation, leading to the invasion rate of cancer cells. In addition, at the high concentrations of TGF-β1, the effect of a matrix metalloproteinase (MMP) inhibitor on the cancer invasion rate was observed. The higher invasion rate would be achieved through the higher MMP production. CONCLUSIONS: The present model is promising to realize the cancer invasion whose rate can be modified by changing the TGF-β1 concentration. |
format | Online Article Text |
id | pubmed-7058408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Japanese Society for Regenerative Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-70584082020-03-09 A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system Nii, Teruki Makino, Kimiko Tabata, Yasuhiko Regen Ther Original Article INTRODUCTION: The objective of this study is to design a cancer invasion model where the cancer invasion rate can be regulated in vitro. METHODS: Cancer-associated fibroblasts (CAF) aggregates incorporating gelatin hydrogel microspheres (GM) containing various concentrations of transforming growth factor-β1 (TGF-β1) (CAF-GM-TGF-β1) were prepared. Alpha-smooth muscle actin (α-SMA) for the CAF aggregates was measured to investigate the CAF activation level by changing the concentration of TGF-β1. An invasion assay was performed to evaluate the cancer invasion rate by co-cultured of cancer cells with various CAF-GM-TGF-β1. RESULTS: The expression level of α-SMA for CAF increased with an increased in the TGF-β1 concentration. When co-cultured with various types of CAF-GM-TGF-β1, the cancer invasion rate was well correlated with the α-SMA level. It is conceivable that the TGF-β1 concentration could modify the level of CAF activation, leading to the invasion rate of cancer cells. In addition, at the high concentrations of TGF-β1, the effect of a matrix metalloproteinase (MMP) inhibitor on the cancer invasion rate was observed. The higher invasion rate would be achieved through the higher MMP production. CONCLUSIONS: The present model is promising to realize the cancer invasion whose rate can be modified by changing the TGF-β1 concentration. Japanese Society for Regenerative Medicine 2020-03-04 /pmc/articles/PMC7058408/ /pubmed/32154334 http://dx.doi.org/10.1016/j.reth.2020.02.003 Text en © 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Nii, Teruki Makino, Kimiko Tabata, Yasuhiko A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system |
title | A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system |
title_full | A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system |
title_fullStr | A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system |
title_full_unstemmed | A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system |
title_short | A cancer invasion model of cancer-associated fibroblasts aggregates combined with TGF-β1 release system |
title_sort | cancer invasion model of cancer-associated fibroblasts aggregates combined with tgf-β1 release system |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058408/ https://www.ncbi.nlm.nih.gov/pubmed/32154334 http://dx.doi.org/10.1016/j.reth.2020.02.003 |
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