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Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects

OBJECTIVES: Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect th...

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Autores principales: Jaturapaktrarak, Chalisa, Payattikul, Penpan, Lohnoo, Tassanee, Kumsang, Yothin, Laikul, Aree, Pathomsakulwong, Watcharapol, Yurayart, Chompoonek, Tonpitak, Walaiporn, Krajaejun, Theerapong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059324/
https://www.ncbi.nlm.nih.gov/pubmed/32143691
http://dx.doi.org/10.1186/s13104-020-04981-y
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author Jaturapaktrarak, Chalisa
Payattikul, Penpan
Lohnoo, Tassanee
Kumsang, Yothin
Laikul, Aree
Pathomsakulwong, Watcharapol
Yurayart, Chompoonek
Tonpitak, Walaiporn
Krajaejun, Theerapong
author_facet Jaturapaktrarak, Chalisa
Payattikul, Penpan
Lohnoo, Tassanee
Kumsang, Yothin
Laikul, Aree
Pathomsakulwong, Watcharapol
Yurayart, Chompoonek
Tonpitak, Walaiporn
Krajaejun, Theerapong
author_sort Jaturapaktrarak, Chalisa
collection PubMed
description OBJECTIVES: Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti-P. insidiosum antibody in multiple host species. RESULTS: A total of 25 pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti-P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile, feasible-to-develop, and functional immunodiagnostic assay for pythiosis.
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spelling pubmed-70593242020-03-12 Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects Jaturapaktrarak, Chalisa Payattikul, Penpan Lohnoo, Tassanee Kumsang, Yothin Laikul, Aree Pathomsakulwong, Watcharapol Yurayart, Chompoonek Tonpitak, Walaiporn Krajaejun, Theerapong BMC Res Notes Research Note OBJECTIVES: Pythiosis is a deadly infectious disease caused by Pythium insidiosum. Reports of both human and animal pythiosis are on the rise worldwide. Prognosis of the pythiosis patients relies on early diagnosis and prompt treatment. There are needs for an immunodiagnostic test that can detect the disease in both humans and animals. This study aims at reporting an optimized protocol for the development of a protein A/G-based enzyme-linked immunosorbent assay (ELISA) for the detection of anti-P. insidiosum antibody in multiple host species. RESULTS: A total of 25 pythiosis and 50 control sera, obtained from humans, horses, dogs, cats, and cows, were recruited for the assay development. With a proper ELISA cutoff point, all pythiosis sera can ultimately be distinguished from the control sera. The successfully-developed protein A/G-based ELISA can detect the anti-P. insidiosum antibodies in serum samples of both humans and animals. It is a versatile, feasible-to-develop, and functional immunodiagnostic assay for pythiosis. BioMed Central 2020-03-06 /pmc/articles/PMC7059324/ /pubmed/32143691 http://dx.doi.org/10.1186/s13104-020-04981-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Jaturapaktrarak, Chalisa
Payattikul, Penpan
Lohnoo, Tassanee
Kumsang, Yothin
Laikul, Aree
Pathomsakulwong, Watcharapol
Yurayart, Chompoonek
Tonpitak, Walaiporn
Krajaejun, Theerapong
Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
title Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
title_full Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
title_fullStr Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
title_full_unstemmed Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
title_short Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects
title_sort protein a/g-based enzyme-linked immunosorbent assay for detection of anti-pythium insidiosum antibodies in human and animal subjects
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059324/
https://www.ncbi.nlm.nih.gov/pubmed/32143691
http://dx.doi.org/10.1186/s13104-020-04981-y
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