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Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019

BACKGROUND: Duck circovirus (DuCV) is a potential immunosuppressive virus that causes feather disorders in young ducks. In this study, DuCV obtained from various species of ducks was investigated by polymerase chain reaction (PCR) in southern and southwestern China (Guangdong, Guangxi and Yunnan pro...

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Autores principales: Liu, Hao, Li, Li Xia, Sun, Wen Chao, Shi, Ning, Sun, Xiu Tao, Jin, Ning Yi, Si, Xing Kui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059369/
https://www.ncbi.nlm.nih.gov/pubmed/32138728
http://dx.doi.org/10.1186/s12917-020-02301-x
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author Liu, Hao
Li, Li Xia
Sun, Wen Chao
Shi, Ning
Sun, Xiu Tao
Jin, Ning Yi
Si, Xing Kui
author_facet Liu, Hao
Li, Li Xia
Sun, Wen Chao
Shi, Ning
Sun, Xiu Tao
Jin, Ning Yi
Si, Xing Kui
author_sort Liu, Hao
collection PubMed
description BACKGROUND: Duck circovirus (DuCV) is a potential immunosuppressive virus that causes feather disorders in young ducks. In this study, DuCV obtained from various species of ducks was investigated by polymerase chain reaction (PCR) in southern and southwestern China (Guangdong, Guangxi and Yunnan provinces) from 2018 to 2019. RESULTS: A total of 848 bursa samples were collected from dead Mulard, Cherry Valley Pekin, Muscovy and Mallard ducks from duck farms. The positivity rate of DuCV in the total sample was approximately 36.91%. We found that the prevalence of DuCV in Yunnan (43.09%) was higher than those in Guangxi (34.38%) and Guangdong (34.4%). However, the positivity rates of DuCV in the four duck species were not significantly different (P > 0.05). Nineteen randomly selected complete viral genomes were sequenced. The complete genomes of the DuCV were 1987 to 1995 nt in length, and were 81.7–99.3% homologous to the other 57 sequences in GenBank. Phylogenetic analyses based on the complete genomes of 76 DuCVs showed that the 19 novel DuCV sequences from Guangdong and Guangxi provinces mainly belonged to the DuCV-1 and DuCV-2 genetic groups, respectively. However, the two genotype groups coexisted in Yunnan Province. In addition, recombination analysis showed putative recombination sites in 3 strains in Yunnan that originated from strains Guangdong and Guangxi. Interestingly, the epidemiological investigation showed that Mulard ducks, Cherry Valley Pekin ducks and Muscovy ducks more than 4 weeks old were more susceptible to infection with the novel DuCV than ducks less than 4 weeks old. CONCLUSIONS: These data provide insight into the molecular epidemiology and genetic diversity of DuCVs circulating in southern and southwestern China for the first time.
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spelling pubmed-70593692020-03-12 Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019 Liu, Hao Li, Li Xia Sun, Wen Chao Shi, Ning Sun, Xiu Tao Jin, Ning Yi Si, Xing Kui BMC Vet Res Research Article BACKGROUND: Duck circovirus (DuCV) is a potential immunosuppressive virus that causes feather disorders in young ducks. In this study, DuCV obtained from various species of ducks was investigated by polymerase chain reaction (PCR) in southern and southwestern China (Guangdong, Guangxi and Yunnan provinces) from 2018 to 2019. RESULTS: A total of 848 bursa samples were collected from dead Mulard, Cherry Valley Pekin, Muscovy and Mallard ducks from duck farms. The positivity rate of DuCV in the total sample was approximately 36.91%. We found that the prevalence of DuCV in Yunnan (43.09%) was higher than those in Guangxi (34.38%) and Guangdong (34.4%). However, the positivity rates of DuCV in the four duck species were not significantly different (P > 0.05). Nineteen randomly selected complete viral genomes were sequenced. The complete genomes of the DuCV were 1987 to 1995 nt in length, and were 81.7–99.3% homologous to the other 57 sequences in GenBank. Phylogenetic analyses based on the complete genomes of 76 DuCVs showed that the 19 novel DuCV sequences from Guangdong and Guangxi provinces mainly belonged to the DuCV-1 and DuCV-2 genetic groups, respectively. However, the two genotype groups coexisted in Yunnan Province. In addition, recombination analysis showed putative recombination sites in 3 strains in Yunnan that originated from strains Guangdong and Guangxi. Interestingly, the epidemiological investigation showed that Mulard ducks, Cherry Valley Pekin ducks and Muscovy ducks more than 4 weeks old were more susceptible to infection with the novel DuCV than ducks less than 4 weeks old. CONCLUSIONS: These data provide insight into the molecular epidemiology and genetic diversity of DuCVs circulating in southern and southwestern China for the first time. BioMed Central 2020-03-05 /pmc/articles/PMC7059369/ /pubmed/32138728 http://dx.doi.org/10.1186/s12917-020-02301-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Liu, Hao
Li, Li Xia
Sun, Wen Chao
Shi, Ning
Sun, Xiu Tao
Jin, Ning Yi
Si, Xing Kui
Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019
title Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019
title_full Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019
title_fullStr Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019
title_full_unstemmed Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019
title_short Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019
title_sort molecular survey of duck circovirus infection in poultry in southern and southwestern china during 2018 and 2019
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059369/
https://www.ncbi.nlm.nih.gov/pubmed/32138728
http://dx.doi.org/10.1186/s12917-020-02301-x
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