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Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP)
Human spaceflight endeavors present an opportunity to expand our presence beyond Earth. To this end, it is crucial to understand and diagnose effects of long‐term space travel on the human body. Developing tools for targeted, on‐site detection of specific DNA sequences will allow us to establish res...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059625/ https://www.ncbi.nlm.nih.gov/pubmed/32161905 http://dx.doi.org/10.1096/fba.2019-00088 |
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author | Rubinfien, Julian Atabay, Kutay D. Nichols, Nicole M. Tanner, Nathan A. Pezza, John A. Gray, Michelle M. Wagner, Brandon M. Poppin, Jayme N. Aken, Jordan T. Gleason, Emily J. Foley, Kevin D. Copeland, David Scott Kraves, Sebastian Alvarez Saavedra, Ezequiel |
author_facet | Rubinfien, Julian Atabay, Kutay D. Nichols, Nicole M. Tanner, Nathan A. Pezza, John A. Gray, Michelle M. Wagner, Brandon M. Poppin, Jayme N. Aken, Jordan T. Gleason, Emily J. Foley, Kevin D. Copeland, David Scott Kraves, Sebastian Alvarez Saavedra, Ezequiel |
author_sort | Rubinfien, Julian |
collection | PubMed |
description | Human spaceflight endeavors present an opportunity to expand our presence beyond Earth. To this end, it is crucial to understand and diagnose effects of long‐term space travel on the human body. Developing tools for targeted, on‐site detection of specific DNA sequences will allow us to establish research and diagnostics platforms that will benefit space programs. We describe a simple DNA diagnostic method that utilizes colorimetric loop‐mediated isothermal amplification (LAMP) to enable detection of a repetitive telomeric DNA sequence in as little as 30 minutes. A proof of concept assay for this method was carried out using existing hardware on the International Space Station and the results were read instantly by an astronaut through a simple color change of the reaction mixture. LAMP offers a novel platform for on‐orbit DNA‐based diagnostics that can be deployed on the International Space Station and to the broader benefit of space programs. |
format | Online Article Text |
id | pubmed-7059625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70596252020-03-11 Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) Rubinfien, Julian Atabay, Kutay D. Nichols, Nicole M. Tanner, Nathan A. Pezza, John A. Gray, Michelle M. Wagner, Brandon M. Poppin, Jayme N. Aken, Jordan T. Gleason, Emily J. Foley, Kevin D. Copeland, David Scott Kraves, Sebastian Alvarez Saavedra, Ezequiel FASEB Bioadv Research Articles Human spaceflight endeavors present an opportunity to expand our presence beyond Earth. To this end, it is crucial to understand and diagnose effects of long‐term space travel on the human body. Developing tools for targeted, on‐site detection of specific DNA sequences will allow us to establish research and diagnostics platforms that will benefit space programs. We describe a simple DNA diagnostic method that utilizes colorimetric loop‐mediated isothermal amplification (LAMP) to enable detection of a repetitive telomeric DNA sequence in as little as 30 minutes. A proof of concept assay for this method was carried out using existing hardware on the International Space Station and the results were read instantly by an astronaut through a simple color change of the reaction mixture. LAMP offers a novel platform for on‐orbit DNA‐based diagnostics that can be deployed on the International Space Station and to the broader benefit of space programs. John Wiley and Sons Inc. 2020-01-16 /pmc/articles/PMC7059625/ /pubmed/32161905 http://dx.doi.org/10.1096/fba.2019-00088 Text en © 2019 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Rubinfien, Julian Atabay, Kutay D. Nichols, Nicole M. Tanner, Nathan A. Pezza, John A. Gray, Michelle M. Wagner, Brandon M. Poppin, Jayme N. Aken, Jordan T. Gleason, Emily J. Foley, Kevin D. Copeland, David Scott Kraves, Sebastian Alvarez Saavedra, Ezequiel Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) |
title | Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) |
title_full | Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) |
title_fullStr | Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) |
title_full_unstemmed | Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) |
title_short | Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) |
title_sort | nucleic acid detection aboard the international space station by colorimetric loop‐mediated isothermal amplification (lamp) |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059625/ https://www.ncbi.nlm.nih.gov/pubmed/32161905 http://dx.doi.org/10.1096/fba.2019-00088 |
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