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Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I(Q) site of mitochondrial complex I
Pseudomonas aeruginosa is a Gram‐negative bacterium of the proteobacteria class, and one of the most common causes of nosocomial infections. For example, it causes chronic pneumonia in cystic fibrosis patients. Patient sputum contains 2‐heptyl‐4‐hydroxyquinoline N‐oxide [HQNO] and Pseudomonas quorum...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059627/ https://www.ncbi.nlm.nih.gov/pubmed/32161908 http://dx.doi.org/10.1096/fba.2019-00084 |
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author | Rieger, Bettina Thierbach, Sven Ommer, Miriam Dienhart, Finja S.V. Fetzner, Susanne Busch, Karin B. |
author_facet | Rieger, Bettina Thierbach, Sven Ommer, Miriam Dienhart, Finja S.V. Fetzner, Susanne Busch, Karin B. |
author_sort | Rieger, Bettina |
collection | PubMed |
description | Pseudomonas aeruginosa is a Gram‐negative bacterium of the proteobacteria class, and one of the most common causes of nosocomial infections. For example, it causes chronic pneumonia in cystic fibrosis patients. Patient sputum contains 2‐heptyl‐4‐hydroxyquinoline N‐oxide [HQNO] and Pseudomonas quorum sensing molecules such as the Pseudomonas quinolone signal [PQS]. It is known that HQNO inhibits the enzyme activity of mitochondrial and bacterial complex III at the Q(i) (quinone reduction) site, but the target of PQS is not known. In this work we have shown that PQS has a negative effect on mitochondrial respiration in HeLa and A549 cells. It specifically inhibits the complex I of the respiratory chain. In vitro analyses showed a partially competitive inhibition with respect to ubiquinone at the I(Q) site. In competing studies with Rotenone, PQS suppressed the ROS‐promoting effect of Rotenone, which is typical for a B‐type inhibitor. Prolonged incubation with PQS also had an effect on the activity of complex III. |
format | Online Article Text |
id | pubmed-7059627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70596272020-03-11 Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I(Q) site of mitochondrial complex I Rieger, Bettina Thierbach, Sven Ommer, Miriam Dienhart, Finja S.V. Fetzner, Susanne Busch, Karin B. FASEB Bioadv Research Articles Pseudomonas aeruginosa is a Gram‐negative bacterium of the proteobacteria class, and one of the most common causes of nosocomial infections. For example, it causes chronic pneumonia in cystic fibrosis patients. Patient sputum contains 2‐heptyl‐4‐hydroxyquinoline N‐oxide [HQNO] and Pseudomonas quorum sensing molecules such as the Pseudomonas quinolone signal [PQS]. It is known that HQNO inhibits the enzyme activity of mitochondrial and bacterial complex III at the Q(i) (quinone reduction) site, but the target of PQS is not known. In this work we have shown that PQS has a negative effect on mitochondrial respiration in HeLa and A549 cells. It specifically inhibits the complex I of the respiratory chain. In vitro analyses showed a partially competitive inhibition with respect to ubiquinone at the I(Q) site. In competing studies with Rotenone, PQS suppressed the ROS‐promoting effect of Rotenone, which is typical for a B‐type inhibitor. Prolonged incubation with PQS also had an effect on the activity of complex III. John Wiley and Sons Inc. 2020-02-19 /pmc/articles/PMC7059627/ /pubmed/32161908 http://dx.doi.org/10.1096/fba.2019-00084 Text en © 2020 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Research Articles Rieger, Bettina Thierbach, Sven Ommer, Miriam Dienhart, Finja S.V. Fetzner, Susanne Busch, Karin B. Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I(Q) site of mitochondrial complex I |
title |
Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I(Q) site of mitochondrial complex I |
title_full |
Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I(Q) site of mitochondrial complex I |
title_fullStr |
Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I(Q) site of mitochondrial complex I |
title_full_unstemmed |
Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I(Q) site of mitochondrial complex I |
title_short |
Pseudomonas Quinolone Signal molecule PQS behaves like a B Class inhibitor at the I(Q) site of mitochondrial complex I |
title_sort | pseudomonas quinolone signal molecule pqs behaves like a b class inhibitor at the i(q) site of mitochondrial complex i |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059627/ https://www.ncbi.nlm.nih.gov/pubmed/32161908 http://dx.doi.org/10.1096/fba.2019-00084 |
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