Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9

The spatiotemporal control of 3D genome is fundamental for gene regulation, yet it remains challenging to profile high-resolution chromatin structure at cis-regulatory elements (CREs). Using C-terminally biotinylated dCas9, endogenous biotin ligases, and pooled sgRNAs, we describe the dCas9-based CA...

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Detalles Bibliográficos
Autores principales: Liu, Xin, Chen, Yong, Zhang, Yuannyu, Liu, Yuxuan, Liu, Nan, Botten, Giovanni A., Cao, Hui, Orkin, Stuart H., Zhang, Michael Q., Xu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059722/
https://www.ncbi.nlm.nih.gov/pubmed/32138752
http://dx.doi.org/10.1186/s13059-020-01973-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059722/
https://www.ncbi.nlm.nih.gov/pubmed/32138752
http://dx.doi.org/10.1186/s13059-020-01973-w

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