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Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9
The spatiotemporal control of 3D genome is fundamental for gene regulation, yet it remains challenging to profile high-resolution chromatin structure at cis-regulatory elements (CREs). Using C-terminally biotinylated dCas9, endogenous biotin ligases, and pooled sgRNAs, we describe the dCas9-based CA...
Autores principales: | Liu, Xin, Chen, Yong, Zhang, Yuannyu, Liu, Yuxuan, Liu, Nan, Botten, Giovanni A., Cao, Hui, Orkin, Stuart H., Zhang, Michael Q., Xu, Jian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059722/ https://www.ncbi.nlm.nih.gov/pubmed/32138752 http://dx.doi.org/10.1186/s13059-020-01973-w |
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