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A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans

Catalase is present in prokaryotic and eukaryotic organisms and is important for the protective effects of the antioxidant system against free radicals. Many studies have confirmed that catalase is required for the growth, development, and pathogenesis of bacteria, plants, animals, and fungi. Howeve...

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Autores principales: Wang, Tu‐Hong, Wang, Xiao‐Wen, Zhu, Xiao‐Qiong, He, Qun, Guo, Li‐Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060140/
https://www.ncbi.nlm.nih.gov/pubmed/31997544
http://dx.doi.org/10.1111/mpp.12907
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author Wang, Tu‐Hong
Wang, Xiao‐Wen
Zhu, Xiao‐Qiong
He, Qun
Guo, Li‐Yun
author_facet Wang, Tu‐Hong
Wang, Xiao‐Wen
Zhu, Xiao‐Qiong
He, Qun
Guo, Li‐Yun
author_sort Wang, Tu‐Hong
collection PubMed
description Catalase is present in prokaryotic and eukaryotic organisms and is important for the protective effects of the antioxidant system against free radicals. Many studies have confirmed that catalase is required for the growth, development, and pathogenesis of bacteria, plants, animals, and fungi. However, there has been relatively little research on the catalases in oomycetes, which form an important group of fungus‐like eukaryotes that produce zoosporangia. In this study, we detected two Phytophthora infestans genes encoding catalases, but only PiCAT2 exhibited catalase activity in the sporulation stage and was highly produced during asexual reproduction and in the late infection stage. Compared with the wild‐type strain, the PiCAT2‐silenced P. infestans transformants were more sensitive to abiotic stress, were less pathogenic, and had a lower colony expansion rate and lower PiMPK7, PiVPS1, and PiGPG1 expression levels. In contrast, the PiCAT2‐overexpressed transformants were slightly less sensitive to abiotic stress. Interestingly, increasing and decreasing PiCAT2 expression from the normal level inhibited sporulation, germination, and infectivity, and down‐regulated PiCdc14 expression, but up‐regulated PiSDA1 expression. These results suggest that PiCAT2 is required for P. infestans mycelial growth, asexual reproduction, abiotic stress tolerance, and pathogenicity. However, a proper PiCAT2 level is critical for the formation and normal function of sporangia. Furthermore, PiCAT2 affects P. infestans sporangial formation and function, pathogenicity, and abiotic stress tolerance by regulating the expression of cell cycle‐related genes (PiCdc14 and PiSDA1) and MAPK pathway genes. Our findings provide new insights into catalase functions in eukaryotic pathogens.
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spelling pubmed-70601402020-03-11 A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans Wang, Tu‐Hong Wang, Xiao‐Wen Zhu, Xiao‐Qiong He, Qun Guo, Li‐Yun Mol Plant Pathol Original Articles Catalase is present in prokaryotic and eukaryotic organisms and is important for the protective effects of the antioxidant system against free radicals. Many studies have confirmed that catalase is required for the growth, development, and pathogenesis of bacteria, plants, animals, and fungi. However, there has been relatively little research on the catalases in oomycetes, which form an important group of fungus‐like eukaryotes that produce zoosporangia. In this study, we detected two Phytophthora infestans genes encoding catalases, but only PiCAT2 exhibited catalase activity in the sporulation stage and was highly produced during asexual reproduction and in the late infection stage. Compared with the wild‐type strain, the PiCAT2‐silenced P. infestans transformants were more sensitive to abiotic stress, were less pathogenic, and had a lower colony expansion rate and lower PiMPK7, PiVPS1, and PiGPG1 expression levels. In contrast, the PiCAT2‐overexpressed transformants were slightly less sensitive to abiotic stress. Interestingly, increasing and decreasing PiCAT2 expression from the normal level inhibited sporulation, germination, and infectivity, and down‐regulated PiCdc14 expression, but up‐regulated PiSDA1 expression. These results suggest that PiCAT2 is required for P. infestans mycelial growth, asexual reproduction, abiotic stress tolerance, and pathogenicity. However, a proper PiCAT2 level is critical for the formation and normal function of sporangia. Furthermore, PiCAT2 affects P. infestans sporangial formation and function, pathogenicity, and abiotic stress tolerance by regulating the expression of cell cycle‐related genes (PiCdc14 and PiSDA1) and MAPK pathway genes. Our findings provide new insights into catalase functions in eukaryotic pathogens. John Wiley and Sons Inc. 2020-01-29 /pmc/articles/PMC7060140/ /pubmed/31997544 http://dx.doi.org/10.1111/mpp.12907 Text en © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Wang, Tu‐Hong
Wang, Xiao‐Wen
Zhu, Xiao‐Qiong
He, Qun
Guo, Li‐Yun
A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans
title A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans
title_full A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans
title_fullStr A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans
title_full_unstemmed A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans
title_short A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans
title_sort proper picat2 level is critical for sporulation, sporangium function, and pathogenicity of phytophthora infestans
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060140/
https://www.ncbi.nlm.nih.gov/pubmed/31997544
http://dx.doi.org/10.1111/mpp.12907
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