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Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study

BACKGROUND: For colorectal cancer (CRC) patients, the standard histological lymph node (LN) evaluation has low sensitivity. Our previously developed one-step nucleic acid amplification (OSNA™) assay measures cytokeratin 19 gene expression in whole LNs. We recently showed that 17.6% of pN0 stage II C...

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Autores principales: Itabashi, Michio, Yamamoto, Hirofumi, Tomita, Naohiro, Inomata, Masafumi, Murata, Kohei, Hayashi, Shigeoki, Miyake, Yasuhiro, Igarashi, Seiji, Kato, Takeshi, Noura, Shingo, Furuhata, Tomohisa, Ozawa, Heita, Takemasa, Ichiro, Yasui, Masayoshi, Takeyama, Hiroshi, Okamura, Shu, Ohno, Yuko, Matsuura, Nariaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060165/
https://www.ncbi.nlm.nih.gov/pubmed/31722072
http://dx.doi.org/10.1245/s10434-019-07971-y
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author Itabashi, Michio
Yamamoto, Hirofumi
Tomita, Naohiro
Inomata, Masafumi
Murata, Kohei
Hayashi, Shigeoki
Miyake, Yasuhiro
Igarashi, Seiji
Kato, Takeshi
Noura, Shingo
Furuhata, Tomohisa
Ozawa, Heita
Takemasa, Ichiro
Yasui, Masayoshi
Takeyama, Hiroshi
Okamura, Shu
Ohno, Yuko
Matsuura, Nariaki
author_facet Itabashi, Michio
Yamamoto, Hirofumi
Tomita, Naohiro
Inomata, Masafumi
Murata, Kohei
Hayashi, Shigeoki
Miyake, Yasuhiro
Igarashi, Seiji
Kato, Takeshi
Noura, Shingo
Furuhata, Tomohisa
Ozawa, Heita
Takemasa, Ichiro
Yasui, Masayoshi
Takeyama, Hiroshi
Okamura, Shu
Ohno, Yuko
Matsuura, Nariaki
author_sort Itabashi, Michio
collection PubMed
description BACKGROUND: For colorectal cancer (CRC) patients, the standard histological lymph node (LN) evaluation has low sensitivity. Our previously developed one-step nucleic acid amplification (OSNA™) assay measures cytokeratin 19 gene expression in whole LNs. We recently showed that 17.6% of pN0 stage II CRC patients were OSNA positive, suggesting a correlation between OSNA results and disease recurrence. This multicenter, prospective study investigateed the prognostic value of the OSNA assay for pStage II CRC patients. METHODS: We examined 204 CRC patients who were preoperatively diagnosed as cN0 and cN1 and surgically treated at 11 medical institutions across Japan. Nine patients were excluded, and 195 patients (Stage I: n = 50, Stage II: n = 70, Stage III: n = 75) were examined. All LNs, harvested from patients, were examined histopathologically using one-slice hematoxylin–eosin staining. Furthermore, half of the LNs was examined by the OSNA assay. Patients were classified according to the UICC staging criteria and OSNA results, and the 3-year, disease-free survival (DFS) of each cohort was analyzed. RESULTS: Average 21.2 LNs/patient were subject to pathological examination. Approximately half of all harvested LNs (average, 9.4 LNs/patient) were suitable for the OSNA assay. Significantly lower 3-year DFS rates were observed in pStage (pathological Stage) II OSNA-positive patients than in OSNA-negative patients (p = 0.005). Among all assessed clinical and pathological parameters, only the OSNA result significantly affected 3-year DFS rates in pStage II CRC patients (p = 0.027). CONCLUSIONS: This study shows that OSNA positivity is a risk factor for recurrence of the patients with pStage II CRC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1245/s10434-019-07971-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-70601652020-03-23 Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study Itabashi, Michio Yamamoto, Hirofumi Tomita, Naohiro Inomata, Masafumi Murata, Kohei Hayashi, Shigeoki Miyake, Yasuhiro Igarashi, Seiji Kato, Takeshi Noura, Shingo Furuhata, Tomohisa Ozawa, Heita Takemasa, Ichiro Yasui, Masayoshi Takeyama, Hiroshi Okamura, Shu Ohno, Yuko Matsuura, Nariaki Ann Surg Oncol Translational Research and Biomarkers BACKGROUND: For colorectal cancer (CRC) patients, the standard histological lymph node (LN) evaluation has low sensitivity. Our previously developed one-step nucleic acid amplification (OSNA™) assay measures cytokeratin 19 gene expression in whole LNs. We recently showed that 17.6% of pN0 stage II CRC patients were OSNA positive, suggesting a correlation between OSNA results and disease recurrence. This multicenter, prospective study investigateed the prognostic value of the OSNA assay for pStage II CRC patients. METHODS: We examined 204 CRC patients who were preoperatively diagnosed as cN0 and cN1 and surgically treated at 11 medical institutions across Japan. Nine patients were excluded, and 195 patients (Stage I: n = 50, Stage II: n = 70, Stage III: n = 75) were examined. All LNs, harvested from patients, were examined histopathologically using one-slice hematoxylin–eosin staining. Furthermore, half of the LNs was examined by the OSNA assay. Patients were classified according to the UICC staging criteria and OSNA results, and the 3-year, disease-free survival (DFS) of each cohort was analyzed. RESULTS: Average 21.2 LNs/patient were subject to pathological examination. Approximately half of all harvested LNs (average, 9.4 LNs/patient) were suitable for the OSNA assay. Significantly lower 3-year DFS rates were observed in pStage (pathological Stage) II OSNA-positive patients than in OSNA-negative patients (p = 0.005). Among all assessed clinical and pathological parameters, only the OSNA result significantly affected 3-year DFS rates in pStage II CRC patients (p = 0.027). CONCLUSIONS: This study shows that OSNA positivity is a risk factor for recurrence of the patients with pStage II CRC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1245/s10434-019-07971-y) contains supplementary material, which is available to authorized users. Springer International Publishing 2019-11-13 2020 /pmc/articles/PMC7060165/ /pubmed/31722072 http://dx.doi.org/10.1245/s10434-019-07971-y Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Translational Research and Biomarkers
Itabashi, Michio
Yamamoto, Hirofumi
Tomita, Naohiro
Inomata, Masafumi
Murata, Kohei
Hayashi, Shigeoki
Miyake, Yasuhiro
Igarashi, Seiji
Kato, Takeshi
Noura, Shingo
Furuhata, Tomohisa
Ozawa, Heita
Takemasa, Ichiro
Yasui, Masayoshi
Takeyama, Hiroshi
Okamura, Shu
Ohno, Yuko
Matsuura, Nariaki
Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study
title Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study
title_full Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study
title_fullStr Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study
title_full_unstemmed Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study
title_short Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study
title_sort lymph node positivity in one-step nucleic acid amplification is a prognostic factor for postoperative cancer recurrence in patients with stage ii colorectal cancer: a prospective, multicenter study
topic Translational Research and Biomarkers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060165/
https://www.ncbi.nlm.nih.gov/pubmed/31722072
http://dx.doi.org/10.1245/s10434-019-07971-y
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