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Resolving dynamics and function of transient states in single enzyme molecules

We use a hybrid fluorescence spectroscopic toolkit to monitor T4 Lysozyme (T4L) in action by unraveling the kinetic and dynamic interplay of the conformational states. In particular, by combining single-molecule and ensemble multiparameter fluorescence detection, EPR spectroscopy, mutagenesis, and F...

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Detalles Bibliográficos
Autores principales:	Sanabria, Hugo, Rodnin, Dmitro, Hemmen, Katherina, Peulen, Thomas-Otavio, Felekyan, Suren, Fleissner, Mark R., Dimura, Mykola, Koberling, Felix, Kühnemuth, Ralf, Hubbell, Wayne, Gohlke, Holger, Seidel, Claus A. M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060211/ https://www.ncbi.nlm.nih.gov/pubmed/32144241 http://dx.doi.org/10.1038/s41467-020-14886-w

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