Cargando…
Pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats
We evaluated pathophysiological characteristics of the lower urinary tract dysfunction in a streptozotocin (STZ)-induced diabetic rat model. STZ (60 mg/kg) was injected intraperitoneally into male Wistar rats. In vitro bladder muscle strip experiments, in vivo cystometry, and simultaneous recordings...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060249/ https://www.ncbi.nlm.nih.gov/pubmed/32144403 http://dx.doi.org/10.1038/s41598-020-61106-y |
_version_ | 1783504193117487104 |
---|---|
author | Masuda, Kazuki Aizawa, Naoki Watanabe, Daiji Okegawa, Takatsugu Kume, Haruki Igawa, Yasuhiko Fukuhara, Hiroshi |
author_facet | Masuda, Kazuki Aizawa, Naoki Watanabe, Daiji Okegawa, Takatsugu Kume, Haruki Igawa, Yasuhiko Fukuhara, Hiroshi |
author_sort | Masuda, Kazuki |
collection | PubMed |
description | We evaluated pathophysiological characteristics of the lower urinary tract dysfunction in a streptozotocin (STZ)-induced diabetic rat model. STZ (60 mg/kg) was injected intraperitoneally into male Wistar rats. In vitro bladder muscle strip experiments, in vivo cystometry, and simultaneous recordings of bladder pressure + urethral perfusion pressure (BP + UPP) with or without intravenous administration of L-arginine (300 mg/kg) or tadalafil (0.03 mg/kg) were performed at several time points. In vitro muscle strip experiments demonstrated that diabetic rats had significantly higher contractile responses to carbachol at 4–16 weeks, and a tendency for higher contractile responses to electrical field stimulation at 4–12 weeks, but this was reversed at 16 weeks. Diabetic rats had significant increases in voided volume, residual volume, bladder capacity, maximal voiding pressure, and amplitude and frequency of non-voiding contractions at 16 weeks. Tadalafil decreased the residual volume in diabetic rats. Diabetic rats had significantly higher UPP nadir and mean UPP during high-frequency oscillation at 16 weeks, which were reversed by tadalafil or L-arginine administration. The present results suggest that urethral relaxation failure, probably related to impairment of the NO/cGMP signalling pathway, rather than bladder contractile dysfunction may be a prominent cause for voiding dysfunction in STZ-induced chronic diabetic rats. |
format | Online Article Text |
id | pubmed-7060249 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70602492020-03-18 Pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats Masuda, Kazuki Aizawa, Naoki Watanabe, Daiji Okegawa, Takatsugu Kume, Haruki Igawa, Yasuhiko Fukuhara, Hiroshi Sci Rep Article We evaluated pathophysiological characteristics of the lower urinary tract dysfunction in a streptozotocin (STZ)-induced diabetic rat model. STZ (60 mg/kg) was injected intraperitoneally into male Wistar rats. In vitro bladder muscle strip experiments, in vivo cystometry, and simultaneous recordings of bladder pressure + urethral perfusion pressure (BP + UPP) with or without intravenous administration of L-arginine (300 mg/kg) or tadalafil (0.03 mg/kg) were performed at several time points. In vitro muscle strip experiments demonstrated that diabetic rats had significantly higher contractile responses to carbachol at 4–16 weeks, and a tendency for higher contractile responses to electrical field stimulation at 4–12 weeks, but this was reversed at 16 weeks. Diabetic rats had significant increases in voided volume, residual volume, bladder capacity, maximal voiding pressure, and amplitude and frequency of non-voiding contractions at 16 weeks. Tadalafil decreased the residual volume in diabetic rats. Diabetic rats had significantly higher UPP nadir and mean UPP during high-frequency oscillation at 16 weeks, which were reversed by tadalafil or L-arginine administration. The present results suggest that urethral relaxation failure, probably related to impairment of the NO/cGMP signalling pathway, rather than bladder contractile dysfunction may be a prominent cause for voiding dysfunction in STZ-induced chronic diabetic rats. Nature Publishing Group UK 2020-03-06 /pmc/articles/PMC7060249/ /pubmed/32144403 http://dx.doi.org/10.1038/s41598-020-61106-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Masuda, Kazuki Aizawa, Naoki Watanabe, Daiji Okegawa, Takatsugu Kume, Haruki Igawa, Yasuhiko Fukuhara, Hiroshi Pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats |
title | Pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats |
title_full | Pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats |
title_fullStr | Pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats |
title_full_unstemmed | Pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats |
title_short | Pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats |
title_sort | pathophysiological changes of the lower urinary tract behind voiding dysfunction in streptozotocin-induced long-term diabetic rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060249/ https://www.ncbi.nlm.nih.gov/pubmed/32144403 http://dx.doi.org/10.1038/s41598-020-61106-y |
work_keys_str_mv | AT masudakazuki pathophysiologicalchangesofthelowerurinarytractbehindvoidingdysfunctioninstreptozotocininducedlongtermdiabeticrats AT aizawanaoki pathophysiologicalchangesofthelowerurinarytractbehindvoidingdysfunctioninstreptozotocininducedlongtermdiabeticrats AT watanabedaiji pathophysiologicalchangesofthelowerurinarytractbehindvoidingdysfunctioninstreptozotocininducedlongtermdiabeticrats AT okegawatakatsugu pathophysiologicalchangesofthelowerurinarytractbehindvoidingdysfunctioninstreptozotocininducedlongtermdiabeticrats AT kumeharuki pathophysiologicalchangesofthelowerurinarytractbehindvoidingdysfunctioninstreptozotocininducedlongtermdiabeticrats AT igawayasuhiko pathophysiologicalchangesofthelowerurinarytractbehindvoidingdysfunctioninstreptozotocininducedlongtermdiabeticrats AT fukuharahiroshi pathophysiologicalchangesofthelowerurinarytractbehindvoidingdysfunctioninstreptozotocininducedlongtermdiabeticrats |