Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs

Increased fidelity mutants of the SpCas9 nuclease constitute the most promising approach to mitigating its off-target effects. However, these variants are effective only in a restricted target space, and many of them are reported to work less efficiently when applied in clinically relevant, pre-asse...

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Detalles Bibliográficos
Autores principales: Kulcsár, Péter István, Tálas, András, Tóth, Eszter, Nyeste, Antal, Ligeti, Zoltán, Welker, Zsombor, Welker, Ervin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060260/
https://www.ncbi.nlm.nih.gov/pubmed/32144253
http://dx.doi.org/10.1038/s41467-020-15021-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060260/
https://www.ncbi.nlm.nih.gov/pubmed/32144253
http://dx.doi.org/10.1038/s41467-020-15021-5

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