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Angiotensin-[1–7] attenuates kidney injury in experimental Alport syndrome

Angiotensin-[1–7] (Ang-[1–7]) antagonize the actions of the renin-angiotensin-system via the Mas receptor and thereby exert renoprotective effects. Murine recombinant angiotensin-converting enzyme (ACE)2 was reported to show renoprotective effects in an experimental Alport syndrome model; however, t...

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Autores principales: Choi, Hong Sang, Kim, In Jin, Kim, Chang Seong, Ma, Seong Kwon, Scholey, James W., Kim, Soo Wan, Bae, Eun Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060323/
https://www.ncbi.nlm.nih.gov/pubmed/32144368
http://dx.doi.org/10.1038/s41598-020-61250-5
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author Choi, Hong Sang
Kim, In Jin
Kim, Chang Seong
Ma, Seong Kwon
Scholey, James W.
Kim, Soo Wan
Bae, Eun Hui
author_facet Choi, Hong Sang
Kim, In Jin
Kim, Chang Seong
Ma, Seong Kwon
Scholey, James W.
Kim, Soo Wan
Bae, Eun Hui
author_sort Choi, Hong Sang
collection PubMed
description Angiotensin-[1–7] (Ang-[1–7]) antagonize the actions of the renin-angiotensin-system via the Mas receptor and thereby exert renoprotective effects. Murine recombinant angiotensin-converting enzyme (ACE)2 was reported to show renoprotective effects in an experimental Alport syndrome model; however, the protective effect of direct administration of Ang-[1–7] is unknown. Here, we used Col4a3(−/−) mice as a model of Alport syndrome, which were treated with saline or Ang- [1–7]; saline-treated wild-type mice were used as a control group. The mice were continuously infused with saline or Ang-[1–7] (25 μg/kg/h) using osmotic mini-pumps. Col4a3(−/−) mice showed increased α-smooth muscle actin (SMA), collagen, and fibronectin expression levels, which were attenuated by Ang-[1–7] treatment. Moreover, Ang-[1–7] alleviated activation of transforming growth factor-β/Smad signaling, and attenuated the protein expression of ED-1 and heme oxygenase-1, indicating reduction of renal inflammation. Ang-[1–7] treatment further reduced the expression levels of inflammatory cytokines and adhesion molecules and attenuated apoptosis in human kidney cells. Finally, Ang-[1–7] downregulated TNF-α converting enzyme and upregulated ACE2 expression. Thus, treatment with Ang-[1–7] altered the ACE2-Ang-[1–7]-Mas receptor axis in the kidneys of Col4a3(−/−) mice to attenuate the nephropathy progression of Alport syndrome.
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spelling pubmed-70603232020-03-18 Angiotensin-[1–7] attenuates kidney injury in experimental Alport syndrome Choi, Hong Sang Kim, In Jin Kim, Chang Seong Ma, Seong Kwon Scholey, James W. Kim, Soo Wan Bae, Eun Hui Sci Rep Article Angiotensin-[1–7] (Ang-[1–7]) antagonize the actions of the renin-angiotensin-system via the Mas receptor and thereby exert renoprotective effects. Murine recombinant angiotensin-converting enzyme (ACE)2 was reported to show renoprotective effects in an experimental Alport syndrome model; however, the protective effect of direct administration of Ang-[1–7] is unknown. Here, we used Col4a3(−/−) mice as a model of Alport syndrome, which were treated with saline or Ang- [1–7]; saline-treated wild-type mice were used as a control group. The mice were continuously infused with saline or Ang-[1–7] (25 μg/kg/h) using osmotic mini-pumps. Col4a3(−/−) mice showed increased α-smooth muscle actin (SMA), collagen, and fibronectin expression levels, which were attenuated by Ang-[1–7] treatment. Moreover, Ang-[1–7] alleviated activation of transforming growth factor-β/Smad signaling, and attenuated the protein expression of ED-1 and heme oxygenase-1, indicating reduction of renal inflammation. Ang-[1–7] treatment further reduced the expression levels of inflammatory cytokines and adhesion molecules and attenuated apoptosis in human kidney cells. Finally, Ang-[1–7] downregulated TNF-α converting enzyme and upregulated ACE2 expression. Thus, treatment with Ang-[1–7] altered the ACE2-Ang-[1–7]-Mas receptor axis in the kidneys of Col4a3(−/−) mice to attenuate the nephropathy progression of Alport syndrome. Nature Publishing Group UK 2020-03-06 /pmc/articles/PMC7060323/ /pubmed/32144368 http://dx.doi.org/10.1038/s41598-020-61250-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Choi, Hong Sang
Kim, In Jin
Kim, Chang Seong
Ma, Seong Kwon
Scholey, James W.
Kim, Soo Wan
Bae, Eun Hui
Angiotensin-[1–7] attenuates kidney injury in experimental Alport syndrome
title Angiotensin-[1–7] attenuates kidney injury in experimental Alport syndrome
title_full Angiotensin-[1–7] attenuates kidney injury in experimental Alport syndrome
title_fullStr Angiotensin-[1–7] attenuates kidney injury in experimental Alport syndrome
title_full_unstemmed Angiotensin-[1–7] attenuates kidney injury in experimental Alport syndrome
title_short Angiotensin-[1–7] attenuates kidney injury in experimental Alport syndrome
title_sort angiotensin-[1–7] attenuates kidney injury in experimental alport syndrome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060323/
https://www.ncbi.nlm.nih.gov/pubmed/32144368
http://dx.doi.org/10.1038/s41598-020-61250-5
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