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Antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp.

BACKGROUND: Acanthamoeba spp. are ubiquitous pathogens which cause granulomatous amoebic encephalitis and disseminated infection. Moreover, Acanthamoeba spp. infection of the cornea leads to Acanthamoeba keratitis. Our previous study showed that the infection of an eyeball may also take place via th...

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Autores principales: Kot, Karolina, Kosik-Bogacka, Danuta, Kupnicka, Patrycja, Łanocha-Arendarczyk, Natalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060556/
https://www.ncbi.nlm.nih.gov/pubmed/32143731
http://dx.doi.org/10.1186/s13071-020-3979-5
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author Kot, Karolina
Kosik-Bogacka, Danuta
Kupnicka, Patrycja
Łanocha-Arendarczyk, Natalia
author_facet Kot, Karolina
Kosik-Bogacka, Danuta
Kupnicka, Patrycja
Łanocha-Arendarczyk, Natalia
author_sort Kot, Karolina
collection PubMed
description BACKGROUND: Acanthamoeba spp. are ubiquitous pathogens which cause granulomatous amoebic encephalitis and disseminated infection. Moreover, Acanthamoeba spp. infection of the cornea leads to Acanthamoeba keratitis. Our previous study showed that the infection of an eyeball may also take place via the migration of trophozoites through the optic nerve from the brain to the eyes. The aim of the study was to analyze the activity of enzymatic antioxidants and the concentration of non-enzymatic antioxidant in the eyes of immunocompetent and immunocompromised mice with disseminated acanthamoebiasis. RESULTS: In the immunocompetent mice infected with Acanthamoeba spp. we noted a significant decrease in catalase activity at 8 and 16 days post-infection (dpi). Glutathione reductase activity was significantly lower at 16 dpi compared to the control group and glutathione concentration was statistically higher at 24 dpi than in the control group. In the immunosuppressed mice, a statistically significant increase in glutathione concentration in the eye samples was found at 16 dpi compared to those not infected with Acanthamoeba spp. In the immunosuppressed mice infected with Acanthamoeba spp., glutathione peroxidase activity was statistically lower at 8 dpi, and glutathione concentration was statistically significantly higher at 16 dpi compared to the control group. CONCLUSIONS: The inflammatory response in the eyes of hosts with experimental acanthamoebiasis led to changes in the activity of enzymatic antioxidants and the content of non-enzymatic antioxidant. Therefore, the dysregulation of antioxidants may play a role in the pathomechanism of Acanthamoeba eye infection. [Image: see text]
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spelling pubmed-70605562020-03-12 Antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp. Kot, Karolina Kosik-Bogacka, Danuta Kupnicka, Patrycja Łanocha-Arendarczyk, Natalia Parasit Vectors Research BACKGROUND: Acanthamoeba spp. are ubiquitous pathogens which cause granulomatous amoebic encephalitis and disseminated infection. Moreover, Acanthamoeba spp. infection of the cornea leads to Acanthamoeba keratitis. Our previous study showed that the infection of an eyeball may also take place via the migration of trophozoites through the optic nerve from the brain to the eyes. The aim of the study was to analyze the activity of enzymatic antioxidants and the concentration of non-enzymatic antioxidant in the eyes of immunocompetent and immunocompromised mice with disseminated acanthamoebiasis. RESULTS: In the immunocompetent mice infected with Acanthamoeba spp. we noted a significant decrease in catalase activity at 8 and 16 days post-infection (dpi). Glutathione reductase activity was significantly lower at 16 dpi compared to the control group and glutathione concentration was statistically higher at 24 dpi than in the control group. In the immunosuppressed mice, a statistically significant increase in glutathione concentration in the eye samples was found at 16 dpi compared to those not infected with Acanthamoeba spp. In the immunosuppressed mice infected with Acanthamoeba spp., glutathione peroxidase activity was statistically lower at 8 dpi, and glutathione concentration was statistically significantly higher at 16 dpi compared to the control group. CONCLUSIONS: The inflammatory response in the eyes of hosts with experimental acanthamoebiasis led to changes in the activity of enzymatic antioxidants and the content of non-enzymatic antioxidant. Therefore, the dysregulation of antioxidants may play a role in the pathomechanism of Acanthamoeba eye infection. [Image: see text] BioMed Central 2020-03-07 /pmc/articles/PMC7060556/ /pubmed/32143731 http://dx.doi.org/10.1186/s13071-020-3979-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kot, Karolina
Kosik-Bogacka, Danuta
Kupnicka, Patrycja
Łanocha-Arendarczyk, Natalia
Antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp.
title Antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp.
title_full Antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp.
title_fullStr Antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp.
title_full_unstemmed Antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp.
title_short Antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with Acanthamoeba spp.
title_sort antioxidant defense in the eyes of immunocompetent and immunosuppressed mice infected with acanthamoeba spp.
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060556/
https://www.ncbi.nlm.nih.gov/pubmed/32143731
http://dx.doi.org/10.1186/s13071-020-3979-5
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