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Human sperm vitrification: the state of the art

Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and...

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Autores principales: Tao, Yong, Sanger, Erika, Saewu, Arpornrad, Leveille, Marie-Claude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060631/
https://www.ncbi.nlm.nih.gov/pubmed/32145746
http://dx.doi.org/10.1186/s12958-020-00580-5
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author Tao, Yong
Sanger, Erika
Saewu, Arpornrad
Leveille, Marie-Claude
author_facet Tao, Yong
Sanger, Erika
Saewu, Arpornrad
Leveille, Marie-Claude
author_sort Tao, Yong
collection PubMed
description Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers. Sperm vitrification has its own limitations. Firstly, small volume of load is usually plunged to liquid nitrogen to achieve high cooling rate, which makes large volume sample cryopreservation less feasible. Secondly, direct contact with liquid nitrogen increases the potential risk of contamination. Recently, new carriers have been developed to facilitate improved control over the volume and speed, and new strategies have been implemented to minimize the contamination risk. In summary, although sperm vitrification has not yet been applied in routine sperm cryopreservation, its potential as a standard procedure is growing.
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spelling pubmed-70606312020-03-12 Human sperm vitrification: the state of the art Tao, Yong Sanger, Erika Saewu, Arpornrad Leveille, Marie-Claude Reprod Biol Endocrinol Review Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers. Sperm vitrification has its own limitations. Firstly, small volume of load is usually plunged to liquid nitrogen to achieve high cooling rate, which makes large volume sample cryopreservation less feasible. Secondly, direct contact with liquid nitrogen increases the potential risk of contamination. Recently, new carriers have been developed to facilitate improved control over the volume and speed, and new strategies have been implemented to minimize the contamination risk. In summary, although sperm vitrification has not yet been applied in routine sperm cryopreservation, its potential as a standard procedure is growing. BioMed Central 2020-03-07 /pmc/articles/PMC7060631/ /pubmed/32145746 http://dx.doi.org/10.1186/s12958-020-00580-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Review
Tao, Yong
Sanger, Erika
Saewu, Arpornrad
Leveille, Marie-Claude
Human sperm vitrification: the state of the art
title Human sperm vitrification: the state of the art
title_full Human sperm vitrification: the state of the art
title_fullStr Human sperm vitrification: the state of the art
title_full_unstemmed Human sperm vitrification: the state of the art
title_short Human sperm vitrification: the state of the art
title_sort human sperm vitrification: the state of the art
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060631/
https://www.ncbi.nlm.nih.gov/pubmed/32145746
http://dx.doi.org/10.1186/s12958-020-00580-5
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