Cargando…
MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4
BACKGROUND: Recent studies showed that aberrant expression of miRNAs causes tumor-suppressing or promoting effects in various cancers including gastric cancer (GC). Our previous studies showed that lots of miRNAs and mRNA expressed differentially in GC and normal tissues. However, the critical miRNA...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060782/ https://www.ncbi.nlm.nih.gov/pubmed/32184620 http://dx.doi.org/10.2147/OTT.S208024 |
_version_ | 1783504294167707648 |
---|---|
author | Wang, Zhu Li, Yang Cao, Jian Zhang, Wei Wang, Quan Zhang, Zhen Gao, Zhidong Ye, Yingjiang Jiang, Kewei Wang, Shan |
author_facet | Wang, Zhu Li, Yang Cao, Jian Zhang, Wei Wang, Quan Zhang, Zhen Gao, Zhidong Ye, Yingjiang Jiang, Kewei Wang, Shan |
author_sort | Wang, Zhu |
collection | PubMed |
description | BACKGROUND: Recent studies showed that aberrant expression of miRNAs causes tumor-suppressing or promoting effects in various cancers including gastric cancer (GC). Our previous studies showed that lots of miRNAs and mRNA expressed differentially in GC and normal tissues. However, the critical miRNAs and mRNA need to be clarified. MATERIALS AND METHODS: Microarray sequencing was used to profile the differential expression of miRNAs and mRNA in GC and normal tissues. Bioinformatics analysis and database prediction were used to search the critical miRNAs and mRNA. Real-time quantitative polymerase chain reaction (RT-qPCR), luciferase reporter assay, immunohistochemistry (IHC), wound healing assay and transwell assay were used to clarify the relationship between the target miRNAs and mRNA. Statistical analysis was used to seek their value of diagnosis and prognosis. RESULTS: We identified microRNA-6165 (miR-6165) as a novel cancer-related miRNA in GC through high-throughput microarray sequencing. By bioinformatics analysis and luciferase reporter assay, we found STRN4 was the target of miR-6165. Via a series of cell experiments, we determined that miR-6165 suppressed GC cells migration and invasion by targeting STRN4. Also, we discovered the potential diagnosis and prognosis value of miR-6165 and STRN4. CONCLUSION: It was found that miR-6165 might suppress GC migration and invasion by targeting STRN4 in vitro, and the further research should focus more on the potential diagnosis and prognosis value of miR-6165 and STRN4 in gastric cancer patients. |
format | Online Article Text |
id | pubmed-7060782 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-70607822020-03-17 MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4 Wang, Zhu Li, Yang Cao, Jian Zhang, Wei Wang, Quan Zhang, Zhen Gao, Zhidong Ye, Yingjiang Jiang, Kewei Wang, Shan Onco Targets Ther Original Research BACKGROUND: Recent studies showed that aberrant expression of miRNAs causes tumor-suppressing or promoting effects in various cancers including gastric cancer (GC). Our previous studies showed that lots of miRNAs and mRNA expressed differentially in GC and normal tissues. However, the critical miRNAs and mRNA need to be clarified. MATERIALS AND METHODS: Microarray sequencing was used to profile the differential expression of miRNAs and mRNA in GC and normal tissues. Bioinformatics analysis and database prediction were used to search the critical miRNAs and mRNA. Real-time quantitative polymerase chain reaction (RT-qPCR), luciferase reporter assay, immunohistochemistry (IHC), wound healing assay and transwell assay were used to clarify the relationship between the target miRNAs and mRNA. Statistical analysis was used to seek their value of diagnosis and prognosis. RESULTS: We identified microRNA-6165 (miR-6165) as a novel cancer-related miRNA in GC through high-throughput microarray sequencing. By bioinformatics analysis and luciferase reporter assay, we found STRN4 was the target of miR-6165. Via a series of cell experiments, we determined that miR-6165 suppressed GC cells migration and invasion by targeting STRN4. Also, we discovered the potential diagnosis and prognosis value of miR-6165 and STRN4. CONCLUSION: It was found that miR-6165 might suppress GC migration and invasion by targeting STRN4 in vitro, and the further research should focus more on the potential diagnosis and prognosis value of miR-6165 and STRN4 in gastric cancer patients. Dove 2020-03-03 /pmc/articles/PMC7060782/ /pubmed/32184620 http://dx.doi.org/10.2147/OTT.S208024 Text en © 2020 Wang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Wang, Zhu Li, Yang Cao, Jian Zhang, Wei Wang, Quan Zhang, Zhen Gao, Zhidong Ye, Yingjiang Jiang, Kewei Wang, Shan MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4 |
title | MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4 |
title_full | MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4 |
title_fullStr | MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4 |
title_full_unstemmed | MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4 |
title_short | MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4 |
title_sort | microrna profile identifies mir-6165 could suppress gastric cancer migration and invasion by targeting strn4 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060782/ https://www.ncbi.nlm.nih.gov/pubmed/32184620 http://dx.doi.org/10.2147/OTT.S208024 |
work_keys_str_mv | AT wangzhu micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT liyang micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT caojian micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT zhangwei micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT wangquan micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT zhangzhen micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT gaozhidong micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT yeyingjiang micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT jiangkewei micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 AT wangshan micrornaprofileidentifiesmir6165couldsuppressgastriccancermigrationandinvasionbytargetingstrn4 |