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Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator

Cardamine violifolia (family Brassicaceae) is the first discovered selenium hyperaccumulator from the genus Cardamine with unique properties in terms of selenium accumulation, i.e., high abundance of selenolanthionine. In our study, a fully comprehensive experiment was conducted with the comparison...

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Autores principales: Both, Eszter Borbála, Stonehouse, Gavin C., Lima, Leonardo Warzea, Fakra, Sirine C., Aguirre, Bernadette, Wangeline, Ami L., Xiang, Jiqian, Yin, Hongqing, Jókai, Zsuzsa, Soós, Áron, Dernovics, Mihály, Pilon-Smits, Elizabeth A.H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060786/
https://www.ncbi.nlm.nih.gov/pubmed/31767332
http://dx.doi.org/10.1016/j.scitotenv.2019.135041
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author Both, Eszter Borbála
Stonehouse, Gavin C.
Lima, Leonardo Warzea
Fakra, Sirine C.
Aguirre, Bernadette
Wangeline, Ami L.
Xiang, Jiqian
Yin, Hongqing
Jókai, Zsuzsa
Soós, Áron
Dernovics, Mihály
Pilon-Smits, Elizabeth A.H.
author_facet Both, Eszter Borbála
Stonehouse, Gavin C.
Lima, Leonardo Warzea
Fakra, Sirine C.
Aguirre, Bernadette
Wangeline, Ami L.
Xiang, Jiqian
Yin, Hongqing
Jókai, Zsuzsa
Soós, Áron
Dernovics, Mihály
Pilon-Smits, Elizabeth A.H.
author_sort Both, Eszter Borbála
collection PubMed
description Cardamine violifolia (family Brassicaceae) is the first discovered selenium hyperaccumulator from the genus Cardamine with unique properties in terms of selenium accumulation, i.e., high abundance of selenolanthionine. In our study, a fully comprehensive experiment was conducted with the comparison of a non-hyperaccumulator Cardamine species, Cardamine pratensis, covering growth characteristics, chlorophyll fluorescence, spatial selenium/sulfur distribution patterns through elemental analyses (synchrotron-based X-Ray Fluorescence and ICP-OES) and speciation data through selenium K-edge micro X-ray absorption near-edge structure analysis (μXANES) and strong cation exchange (SCX)-ICP-MS. The results revealed remarkable differences in contrast to other selenium hyperaccumulators as neither Cardamine species showed evidence of growth stimulation by selenium. Also, selenite uptake was not inhibited by phosphate for either of the Cardamine species. Sulfate inhibited selenate uptake, but the two Cardamine species did not show any difference in this respect. However, μXRF derived speciation maps and selenium/sulfur uptake characteristics provided results that are similar to other formerly reported hyperaccumulator and non-hyperaccumulator Brassicaceae species. μXANES showed organic selenium, “C-Se-C”, in seedlings of both species and also in mature C. violifolia plants. In contrast, selenate-supplied mature C. pratensis contained approximately half “C-Se-C” and half selenate. SCX-ICP-MS data showed evidence of the lack of selenocystine in any of the Cardamine plant extracts. Thus, C. violifolia shows clear selenium-related physiological and biochemical differences compared to C. pratensis and other selenium hyperaccumulators.
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spelling pubmed-70607862020-03-07 Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator Both, Eszter Borbála Stonehouse, Gavin C. Lima, Leonardo Warzea Fakra, Sirine C. Aguirre, Bernadette Wangeline, Ami L. Xiang, Jiqian Yin, Hongqing Jókai, Zsuzsa Soós, Áron Dernovics, Mihály Pilon-Smits, Elizabeth A.H. Sci Total Environ Article Cardamine violifolia (family Brassicaceae) is the first discovered selenium hyperaccumulator from the genus Cardamine with unique properties in terms of selenium accumulation, i.e., high abundance of selenolanthionine. In our study, a fully comprehensive experiment was conducted with the comparison of a non-hyperaccumulator Cardamine species, Cardamine pratensis, covering growth characteristics, chlorophyll fluorescence, spatial selenium/sulfur distribution patterns through elemental analyses (synchrotron-based X-Ray Fluorescence and ICP-OES) and speciation data through selenium K-edge micro X-ray absorption near-edge structure analysis (μXANES) and strong cation exchange (SCX)-ICP-MS. The results revealed remarkable differences in contrast to other selenium hyperaccumulators as neither Cardamine species showed evidence of growth stimulation by selenium. Also, selenite uptake was not inhibited by phosphate for either of the Cardamine species. Sulfate inhibited selenate uptake, but the two Cardamine species did not show any difference in this respect. However, μXRF derived speciation maps and selenium/sulfur uptake characteristics provided results that are similar to other formerly reported hyperaccumulator and non-hyperaccumulator Brassicaceae species. μXANES showed organic selenium, “C-Se-C”, in seedlings of both species and also in mature C. violifolia plants. In contrast, selenate-supplied mature C. pratensis contained approximately half “C-Se-C” and half selenate. SCX-ICP-MS data showed evidence of the lack of selenocystine in any of the Cardamine plant extracts. Thus, C. violifolia shows clear selenium-related physiological and biochemical differences compared to C. pratensis and other selenium hyperaccumulators. 2019-11-02 2020-02-10 /pmc/articles/PMC7060786/ /pubmed/31767332 http://dx.doi.org/10.1016/j.scitotenv.2019.135041 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Both, Eszter Borbála
Stonehouse, Gavin C.
Lima, Leonardo Warzea
Fakra, Sirine C.
Aguirre, Bernadette
Wangeline, Ami L.
Xiang, Jiqian
Yin, Hongqing
Jókai, Zsuzsa
Soós, Áron
Dernovics, Mihály
Pilon-Smits, Elizabeth A.H.
Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator
title Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator
title_full Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator
title_fullStr Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator
title_full_unstemmed Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator
title_short Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator
title_sort selenium tolerance, accumulation, localization and speciation in a cardamine hyperaccumulator and a non-hyperaccumulator
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7060786/
https://www.ncbi.nlm.nih.gov/pubmed/31767332
http://dx.doi.org/10.1016/j.scitotenv.2019.135041
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